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Cell No. : Cell Name
RCB1248 : Anti-α(9)12G8B11  update : 2024/01/17
CommentMoAb to Clostridium perfringens alpha-toxin (phospholipase C).
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
1-33-4Kamiuma Setagaya-ku
Tokyo 154-0011 Japan
Dr.SATO Hiroko
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sato, Hiroko
Originator Sato, Hiroko
Year of deposit 1996
Another name 12G8B11
Animal _mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U856
hybridoma Fusion_cell_1 name spleen cell
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name SP2/0
Fusion_cell_2 strain mouse
Fusion method PEG, HAT selection
Antibody name 12G8B11
Subclass IgG2b
Immunogen C.perfringens α-Toxin and Toxoid
Antigen_name C.perfringens α-Toxin
Antigen_m.w. 42.5kDa
Antigen_character protein
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium DMEM (low glucose) + 20% FBS + 8μg/ml Insulin + 1μg/ml Transferrin + 50μM 2-Mercaptoethanol
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2b
Reference information Reference 1
User's Publication 0

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2315  Sato H, Chiba J, Sato Y.  Monoclonal antibodies against alpha toxin of Clostridium perfringens.  FEMS Microbiol Lett.  1989  50(1-2):173-6  PubMed ID: 2544482   DOI: 10.1016/0378-1097(89)90480-1

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