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Cell No. : Cell Name
RCB1239 : Anti-FHA(1)1C6  update : 2022/03/31
CommentAnti-pertussis filamentous hemagglutinin (FHA) MoAb producing.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
1-33-4Kamiuma Setagaya-ku
Tokyo 154-0011 Japan
Dr.SATO Hiroko
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sato, Hiroko
Originator Sato, Hiroko
Year of deposit 1996
Another name F(1)1C6
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U639
hybridoma Fusion_cell_1 name spleen cell
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name SP2/0
Fusion_cell_2 strain mouse
Fusion method PEG, HAT selection
Antibody name 1C6
Subclass IgG1
Immunogen FHA(filamentous hemagglutinin) + PTd + αtoxoid
Antigen_name FHA(filamentous hemagglutinin)
Antigen_m.w. 220kDa〜
Antigen_character protein
Antigen_epitope FHA
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM (low glucose) + 20% FBS + 8μg/ml Insulin + 1μg/ml Transferrin + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG1
Images
deposit info
lot info
Reference information Reference 4
User's Publication 0


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Reference
5475  Sato Y, Sato H, Kodama H, Uchimura M, Miwa N, Kobayashi T, Yamamoto E, Fujita I, Kumamoto T.  An improved ELISA system for the measurement of IgG antibodies against pertussis toxin (PT) and filamentous hemagglutinin (FHA) in human sera.  Dev Biol Stand.  1991  73:167-74  PubMed ID: 1778310  
5473  Sato H, Sato Y.  Protective antigens of Bordetella pertussis mouse-protection test against intracerebral and aerosol challenge of B. pertussis.  Dev Biol Stand  1985  61:461-7  PubMed ID: 2872127  
5474  Sato Y, Sato H.  Anti-pertussis toxin IgG and anti-filamentous hemagglutinin IgG production in children immunized with pertussis acellular vaccine and comparison of these titers with the sera of pertussis convalescent children.  Dev Biol Stand  1985  61:367-72  PubMed ID: 3835079  
5472  Sato H, Sato Y.  Bordetella pertussis infection in mice: correlation of specific antibodies against two antigens, pertussis toxin, and filamentous hemagglutinin with mouse protectivity in an intracerebral or aerosol challenge system.  Infect Immun.  1984  46(2):415-21  PubMed ID: 6542069  

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