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Cell No. : Cell Name
RCB1238 : Anti-FHA(2)2E9 update : 2024/11/06 |
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| Comment | Anti-pertussis filamentous hemagglutinin (FHA) MoAb producing. | |||
| Comment from the depositor | ||||
| Terms and conditions | A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Sato, Hiroko | ||
| Originator | Sato, Hiroko | |||
| Year of deposit | 1996 | |||
| Another name | F(2)2E9 | |||
| Animal | _mouse x mouse < Hybrid | |||
| Tissue | hybridoma | |||
| Classification | hybrid cells | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Cellosaurus(Expasy) | CVCL_U640 | hybridoma | Fusion_cell_1 name | spleen cell |
| Fusion_cell_1 strain | mouse, BALB/c | |||
| Fusion_cell_2 name | SP2/0 | |||
| Fusion_cell_2 strain | mouse | Fusion method | PEG, HAT selection | Antibody name | 2E9 |
| Subclass | IgG1 | |||
| Immunogen | FHA(filamentous hemagglutinin) + PTd + αtoxoid | Antigen_name | FHA(filamentous hemagglutinin) | |
| Antigen_m.w. | 220kDa〜 | |||
| Antigen_character | protein | |||
| Antigen_epitope | FHA | |||
| Medium | Medium List | Culture type | Suspension cells | |
| Culture method | 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | DMEM (low glucose) + 20% FBS + 8μg/ml Insulin + 1μg/ml Transferrin + 50μM 2-Mercaptoethanol | |||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage ratio | 1 : 10 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Antibody subclass | IgG1 | |||
| Images | ||||
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| Reference information | Reference | 4 | ||
| User's Publication | 0 | |||
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