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Cell No. : Cell Name
RCB1229 : Anti-PT-S4-7F2  update : 2024/01/17
CommentAnti-pertussis toxin subunit S-4 MoAb (IgG1) producing.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
1-33-4Kamiuma Setagaya-ku
Tokyo 154-0011 Japan
Dr.SATO Hiroko
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sato, Hiroko
Originator Sato, Hiroko
Year of deposit 1996
Another name PT(25)7F2
Animal _mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U650
hybridoma Fusion_cell_1 name spleen cell
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name SP2/0
Fusion_cell_2 strain mouse
Fusion method PEG, HAT selection
Antibody name 7F2
Subclass IgG1
Immunogen Pertussis Toxins and Toxoids
Antigen_name Pertussis Toxins (PT)
Antigen_m.w. 106kDa
Antigen_character protein
Antigen_epitope subunit S-4
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium DMEM (low glucose) + 20% FBS + 8μg/ml Insulin + 1μg/ml Transferrin + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Antibody subclass IgG1
Images
deposit info
lot info
Reference information Reference 7
User's Publication 0


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Reference
5471  Sato H, Sato Y.  Relationship between structure and biological and protective activities of pertussis toxin.  Dev Biol Stand  1991  73:121-32  PubMed ID: 1723387  
2169  Sato H, Sato Y, Ohishi I.  Comparison of pertussis toxin (PT)-neutralizing activities and mouse-protective activities of anti-PT mouse monoclonal antibodies.  Infect Immun  1991  59(10):3832-5  PubMed ID: 1894382   DOI: 10.1128/iai.59.10.3832-3835.1991
5475  Sato Y, Sato H, Kodama H, Uchimura M, Miwa N, Kobayashi T, Yamamoto E, Fujita I, Kumamoto T.  An improved ELISA system for the measurement of IgG antibodies against pertussis toxin (PT) and filamentous hemagglutinin (FHA) in human sera.  Dev Biol Stand.  1991  73:167-74  PubMed ID: 1778310  
2314  Sato H, Sato Y.  Protective activities in mice of monoclonal antibodies against pertussis toxin.  Infect Immun  1990  58(10):3369-74  PubMed ID: 1698179   DOI: 10.1128/iai.58.10.3369-3374.1990
5473  Sato H, Sato Y.  Protective antigens of Bordetella pertussis mouse-protection test against intracerebral and aerosol challenge of B. pertussis.  Dev Biol Stand  1985  61:461-7  PubMed ID: 2872127  
5474  Sato Y, Sato H.  Anti-pertussis toxin IgG and anti-filamentous hemagglutinin IgG production in children immunized with pertussis acellular vaccine and comparison of these titers with the sera of pertussis convalescent children.  Dev Biol Stand  1985  61:367-72  PubMed ID: 3835079  
5472  Sato H, Sato Y.  Bordetella pertussis infection in mice: correlation of specific antibodies against two antigens, pertussis toxin, and filamentous hemagglutinin with mouse protectivity in an intracerebral or aerosol challenge system.  Infect Immun.  1984  46(2):415-21  PubMed ID: 6542069   DOI: 10.1128/iai.46.2.415-421.1984

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