Cell No. : Cell Name RCB1200 : MMAc·SF  update : 2022/03/30
Comment			Serum-free medium-adapted MMAc cells. For SF culture, + insulin, hEGF, hydrocortisone, bovine pituit
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Katayama, Hiroshi
		Originator	Katayama, Hiroshi
		Year of deposit	1996
		Animal	human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	39 years
		Tissue	melanoma, ascites meta
		Metastatic ability	Yes
		Metastatic tissue	liver, lymph node et
		Classification	cancer
		Year of origin	1995
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_1420
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Medium and additives		HuMedia-KG2
		Antibiotics		Free
		Passage method		0.25% Trypsin, added HuMedia-KG2 + 10%FBS, washed twice with HuMedia-KG2
	Culture information	Passage ratio		1 : 3-4 split
		SC frequency		Subculture : once/10 days, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		HuMedia : 2 x FM1 = 1 : 1
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Isozyme		LD, NP
		Chromosome mode		69-80(16) : /72(1),72(2),74(1),75(2),76(3),77(3)/
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	0
		User's Publication	3

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User's Publication

20812  Comandante-Lou N, Baumann DG, Fallahi-Sichani M.  AP-1 transcription factor network explains diverse patterns of cellular plasticity in melanoma cells.  Cell Rep  2022  40(5)  PubMed ID: 35926467

13309  Gerosa L, Chidley C, Fröhlich F, Sanchez G, Lim SK, Muhlich J, Chen JY, Vallabhaneni S, Baker GJ, Schapiro D, Atanasova MI, Chylek LA, Shi T, Yi L, Nicora CD, Claas A, Ng TSC, Kohler RH, Lauffenburger DA, Weissleder R, Miller MA, Qian WJ, Wiley HS, Sorger PK.  Receptor-Driven ERK Pulses Reconfigure MAPK Signaling and Enable Persistence of Drug-Adapted BRAF-Mutant Melanoma Cells.  Cell Syst  2020    PubMed ID: 33113355

14264  Ward GA, Lewis EJ, Ahn JS, Johnson CN, Lyons JF, Martins V, Munck JM, Rich SJ, Smyth T, Thompson NT, Williams PA, Wilsher NE, Wallis NG, Chessari G.  ASTX660, a Novel Non-peptidomimetic Antagonist of cIAP1/2 and XIAP, Potently Induces TNFα-Dependent Apoptosis in Cancer Cell Lines and Inhibits Tumor Growth  Mol Cancer Ther  2018  17(7):1381-1391  PubMed ID: 29695633