Cell No. : Cell Name
RCB1197 : P2UR/K-562
update : 2024/01/17
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| Comment | Expressing P2U receptor acting in signal transduction pathway. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Enomoto, Koh-ichi
|
| Originator |
Enomoto, Koh-ichi
|
| Year of deposit |
1996
|
| Original cell |
K-562
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
53 years
|
| Tissue |
pleural effusion
|
| Disease name |
chronic myelogenous leukemia
|
| Classification |
cancer
|
| Recombinant |
recombinant
|
| Exogene |
P2U receptor cDNA , 1259base
|
| Vector |
pCR3
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_L434
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
DMEM (high glucose) + 5% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
LT 400
|
| Virus (HTLV-1) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
63-69(50) : 63(3),64(9),65(14),66(16),67(4),68(3)/
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
0
|
| Reference |
|
8537
K Enomoto, K Furuya, R C Moore, S Yamagishi, T Oka, T Maeno
Expression cloning and signal transduction pathway of P2U receptor in mammary tumor cells
Biol Signals
1996
5(1):9-21
PubMed ID: 8739319
DOI: 10.1159/000109169
|
|
87
Lozzio BB, Lozzio CB, Bamberger EG, Feliu AS.
A multipotential leukemia cell line (K-562) of human origin
Proc Soc Exp Biol Med
1981
166(4):546-50
PubMed ID: 7194480
DOI: 10.3181/00379727-166-41106
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|
969
Rutherford TR, Clegg JB, Weatherall DJ.
K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.
Nature
1979
280(5718):164-5
PubMed ID: 95354
DOI: 10.1038/280164a0
|
|
970
Andersson LC, Jokinen M, Gahmberg CG.
Induction of erythroid differentiation in the human leukaemia cell line K562.
Nature
1979
278(5702):364-5
PubMed ID: 570644
DOI: 10.1038/278364a0
|
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