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Cell No. : Cell Name
RCB1197 : P2UR/K-562  update : 2022/03/30
CommentExpressing P2U receptor acting in signal transduction pathway.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Enomoto, Koh-ichi
Originator Enomoto, Koh-ichi
Year of deposit 1996
Original cell K-562
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 53 years
Tissue pleural effusion
Disease name chronic myelogenous leukemia
Classification cancer
Recombinant recombinant
Exogene P2U receptor cDNA , 1259base
Vector pCR3
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_L434
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM (high glucose) + 5% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 63-69(50) : 63(3),64(9),65(14),66(16),67(4),68(3)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


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Reference
8537  K Enomoto, K Furuya, R C Moore, S Yamagishi, T Oka, T Maeno  Expression cloning and signal transduction pathway of P2U receptor in mammary tumor cells  Biol Signals  1996  5(1):9-21  PubMed ID: 8739319
969  Rutherford TR, Clegg JB, Weatherall DJ.  K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.  Nature  1979  280(5718):164-5  PubMed ID: 95354
970  Andersson LC, Jokinen M, Gahmberg CG.  Induction of erythroid differentiation in the human leukaemia cell line K562.  Nature  1979  278(5702):364-5  PubMed ID: 570644

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