Cell No. : Cell Name
RCB1190 : 7-TD-1
update : 2024/06/12
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| Comment | Responding to IL-6 in the bioassay. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 23 prior to order. |
| Comment from the depositor | |
| Terms and conditions | 1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 23 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
DSMZ
|
| Year of deposit |
1995
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
| History |
overseas
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_1935
|
|
hybridoma
|
Fusion_cell_1 name |
spleen
|
| Fusion_cell_1 strain |
mouse, C57B1/6
|
| Fusion_cell_2 name |
myeloma
|
| Fusion_cell_2 strain |
mouse
|
|
Fusion method |
HAT selection
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|
Antibody name |
IL-6 dependent cell line
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
|
Suspension cells
|
| Culture method |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 5% FBS + 0.025mM 2-Mercaptoethanol + 25U/ml IL-6
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| Antibiotics |
|
Free
|
|
Passage method |
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dilution
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Culture information
|
Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
7
|
| User's Publication |
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16514
Sato Y, Kashiwakura I, Yamaguchi M, Yoshino H, Tanaka T, Ikeda K, Ye Z, Komatsu H, Matsuzaki T, Hosoda M.
Discovery of a Novel Small-molecule Interleukin-6 Inhibitor Through Virtual Screening Using Artificial Intelligence
Med Chem
2022
18(6):694-700
PubMed ID: 34784877
DOI: 10.2174/1573406418666211116144243
|
|
9571
Fujiwara Y, Yang L, Takaiwa F, Sekikawa K.
Expression and Purification of Recombinant Mouse Interleukin-4 and -6 from Transgenic Rice Seeds.
Mol. Biotechnol.
2016
58:223-31
PubMed ID: 26876890
DOI: 10.1007/s12033-016-9920-7
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13977
Mori T, Murasawa Y, Ikai R, Hayakawa T, Nakamura H, Ogiso N, Niida S, Watanabe K.
Generation of a transgenic mouse line for conditional expression of human IL-6
Exp Anim
2016
65(4):455-463
PubMed ID: 27349442
DOI: 10.1538/expanim.16-0043
|
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14163
Hirasawa T, Fujimoto S, Kuratani S.
Expansion of the neck reconstituted the shoulder-diaphragm in amniote evolution
Dev Growth Differ
2016
58(1):143-53
PubMed ID: 26510533
DOI: 10.1111/dgd.12243
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13748
Sakaue Y, Nezu Y, Yanagisawa S, Komori S, Hara Y, Takahashi K, Tagawa M, Ogawa R.
Effects of continuous low-dose infusion of lipopolysaccharide on expression of E-selectin and intercellular adhesion molecule-1 messenger RNA and neutrophil accumulation in specific organs in dogs
Am J Vet Res
2005
66(7):1259-66
PubMed ID: 16111167
DOI: 10.2460/ajvr.2005.66.1259
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3790
Kishikawa H, Miura S, Yoshida H, Hirokawa M, Nakamizo H, Higuchi H, Adachi M, Nakatsumi RC, Suzuki H, Saito H, Ishii H. Author information
Transmural pressure induces IL-6 secretion by intestinal epithelial cells.
Clin Exp Immunol
2002
129(1):86-91
PubMed ID: 12100026
DOI: 10.1046/j.1365-2249.2002.01895.x
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2380
Yokota S, Yanagi H, Yura T, Kubota H
Cytosolic chaperonin is up-regulated during cell growth. Preferential expression and binding to tubulin at G(1)/S transition through early S phase.
J Biol Chem
1999
274:37070-8
PubMed ID: 10601265
DOI: 10.1074/jbc.274.52.37070
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