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Cell No. : Cell Name
RCB1190 : 7-TD-1  update : 2023/03/08
CommentResponding to IL-6 in the bioassay. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 23 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 23 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor DSMZ
Year of deposit 1995
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
History overseas
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1935
hybridoma Fusion_cell_1 name spleen
Fusion_cell_1 strain mouse, C57B1/6
Fusion_cell_2 name myeloma
Fusion_cell_2 strain mouse
Fusion method HAT selection
Antibody name IL-6 dependent cell line
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 5% FBS + 0.025mM 2-Mercaptoethanol + 25U/ml IL-6
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 7

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2034  Van Snick J, Cayphas S, Vink A, Uyttenhove C, Coulie, P G, Rubira, M R, Simpson, R J  Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas.  Proc Natl Acad Sci U S A  1986  83:9679-83  PubMed ID: 2948184   DOI: 10.1073/pnas.83.24.9679

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User's Publication
16514  Sato Y, Kashiwakura I, Yamaguchi M, Yoshino H, Tanaka T, Ikeda K, Ye Z, Komatsu H, Matsuzaki T, Hosoda M.  Discovery of a Novel Small-molecule Interleukin-6 Inhibitor Through Virtual Screening Using Artificial Intelligence  Med Chem  2022  18(6):694-700  PubMed ID: 34784877   DOI: 10.2174/1573406418666211116144243
9571  Fujiwara Y, Yang L, Takaiwa F, Sekikawa K.  Expression and Purification of Recombinant Mouse Interleukin-4 and -6 from Transgenic Rice Seeds.  Mol. Biotechnol.  2016  58:223-31  PubMed ID: 26876890   DOI: 10.1007/s12033-016-9920-7
13977  Mori T, Murasawa Y, Ikai R, Hayakawa T, Nakamura H, Ogiso N, Niida S, Watanabe K.  Generation of a transgenic mouse line for conditional expression of human IL-6  Exp Anim  2016  65(4):455-463  PubMed ID: 27349442   DOI: 10.1538/expanim.16-0043
14163  Hirasawa T, Fujimoto S, Kuratani S.  Expansion of the neck reconstituted the shoulder-diaphragm in amniote evolution  Dev Growth Differ  2016  58(1):143-53  PubMed ID: 26510533   DOI: 10.1111/dgd.12243
13748  Sakaue Y, Nezu Y, Yanagisawa S, Komori S, Hara Y, Takahashi K, Tagawa M, Ogawa R.  Effects of continuous low-dose infusion of lipopolysaccharide on expression of E-selectin and intercellular adhesion molecule-1 messenger RNA and neutrophil accumulation in specific organs in dogs  Am J Vet Res  2005  66(7):1259-66  PubMed ID: 16111167   DOI: 10.2460/ajvr.2005.66.1259
3790  Kishikawa H, Miura S, Yoshida H, Hirokawa M, Nakamizo H, Higuchi H, Adachi M, Nakatsumi RC, Suzuki H, Saito H, Ishii H. Author information  Transmural pressure induces IL-6 secretion by intestinal epithelial cells.  Clin Exp Immunol  2002  129(1):86-91  PubMed ID: 12100026   DOI: 10.1046/j.1365-2249.2002.01895.x
2380  Yokota S, Yanagi H, Yura T, Kubota H  Cytosolic chaperonin is up-regulated during cell growth. Preferential expression and binding to tubulin at G(1)/S transition through early S phase.  J Biol Chem  1999  274:37070-8  PubMed ID: 10601265   DOI: 10.1074/jbc.274.52.37070

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