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Cell No. : Cell Name
RCB1188 : NRS-1  update : 2021/05/20
CommentHuman rhabdomyosarcoma showing myogenic differentiation. TGFbeta inhibits it. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Motoyama, Teiichi
Originator Ogose, Akira & Motoyama, Teiichi
Year of deposit 1995
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 7 years
Tissue left forearm
Disease name alveolar rhabdomyosarcoma
Metastastatic ability Yes
Metastatsd tissue left peroneus
Classification cancer
Year of origin 1992
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4871
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/10 days, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 84-180(50) : /89(6),90(6),91(2),92(6),93(6),94(7)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 4


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Reference
2032  Ogose A, Motoyama T, Hotta T, Watanabe H  In vitro differentiation and proliferation in a newly established human rhabdomyosarcoma cell line.  Virchows Arch  1995  426:385-91  PubMed ID: 7599791

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User's Publication
13263  Kato K, Yoshimi A, Noda A, Otani H, Hojo H, Tanaka M, Tanaka Y, Ito Y, Nishimura R, Takita J, Yanai T, Koike K, Tsuchida M.  Distinct clonal evolution in a case with anaplastic embryonal rhabdomyosarcoma.  Pediatr Int  2021  63(7):782-789  PubMed ID: 33021000
9801  Jaaks P, D'Alessandro V, Grob N, Büel S, Hajdin K, Schäfer BW, Bernasconi M.  The Proprotein Convertase Furin Contributes to Rhabdomyosarcoma Malignancy by Promoting Vascularization, Migration and Invasion.  PLoS ONE  2016  11:e0161396  PubMed ID: 27548722
6474  Yoshitaka T, Kawai A, Miyaki S, Numoto K, Kikuta K, Ozaki T, Lotz M, Asahara H.  Analysis of microRNAs expressions in chondrosarcoma.  J Orthop Res  2013  31(12):1992-8.  PubMed ID: 23940002
6475  Osaki M, Osaki M, Yamashita H, Shomori K, Yoshida H, Ito H.  Expression of minichromosome maintenance-2 in human malignant fibrous histiocytomas: Correlations with Ki-67 and P53 expression, and apoptosis.  Int J Mol Med  2002  10(2):161-8  PubMed ID: 12119552



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