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Cell No. : Cell Name
RCB1187 : TUHR3TKB  update : 2021/05/20
CommentJapanese kidney carcinoma cells. RCB original.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, T. & Kawai, K.
Originator Liu, Shu Qin
Year of deposit 1995
Another name ArMa
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 38 years
Tissue kidney
Classification cancer
Year of origin 1995
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_5956
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/6 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 44-46(21) : 44(1),45(2),46(18)
STR(human) OK
Reference information Reference 1
User's Publication 1


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Reference
4063  Liu SQ, Kawai K, Shiraiwa H, Hayashi H, Akaza H, Hashizaki K, Shiba R, Saijo K, Ohno T.  High rate of induction of human autologous cytotoxic T lymphocytes against renal carcinoma cells cultured with an interleukin cocktail.  Jpn J Cancer Res  1998  89(11):1195-201  PubMed ID: 9914789

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User's Publication
15554  Peng BG, He Q, Liang LI, Xie BH, Hua YP, Chen ZB, Zhou F.  Induction of cytotoxic T-lymphocyte responses using dendritic cells transfected with hepatocellular carcinoma mRNA.  Br J Biomed Sci  2006  63:123-8 



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