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Cell No. : Cell Name
RCB1181 : ST  update : 2022/03/30
CommentA subclone of RCB1180 Tanoue.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Tsuchiya, Hiroyuki
Originator Soheir S. El-Sonbaty
Year of deposit 1995
Original cell Tanoue
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 10 years
Tissue blood
Disease name acute lymphoblastic leukemia
Classification cancer
Year of origin 1991
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U347
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 45-49(50) : 45(2),46(9),47(26),48(10),49(3)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
2031  el-Sonbaty, S S, Tsuchiya H, Watanabe M, Hochito K, Kunisada T, Shimosaka A, Matsuda I  Exogenous expression of human granulocyte colony-stimulating factor receptor in a B-lineage acute lymphoblastic leukemia cell line: a possible model for mixed lineage leukemia.  Leuk Res  1995  19:249-56  PubMed ID: 7538617

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