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Cell No. : Cell Name
RCB1179 : HuG1-N  update : 2024/01/17
CommentHuman cell line derived from ascites of a gastric cancer patient. Expressing Nagao-type alkaline phosphatase. HuG1-PI is another cell line derived from the same patient.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Imanishi, Hiroyasu
Originator Imanishi, Hiroyasu
Year of deposit 1995
Another name HuG
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 55 years
Tissue stomach, ascites meta
Disease name borrmann
Metastatic ability Yes
Metastatic tissue liver,peritoneum
Classification cancer
Year of origin 1986
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_4846
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 57-64(50) : /58(2),59(4),60(12),61(18),62(7),63(5)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 5

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2030  Imanishi H, Hada T, Muratani K, Hirano K, Higashino K  Expression of a hybrid form of alkaline phosphatase isoenzyme in a newly established cell line (HuG-1) from a gastric cancer patient.  Cancer Res  1990  50:3408-12  PubMed ID: 2334935  

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User's Publication
5845  Konno-Shimizu M1, Yamamichi N, Inada K, Kageyama-Yahara N, Shiogama K, Takahashi Y, Asada-Hirayama I, Yamamichi-Nishina M, Nakayama C, Ono S, Kodashima S, Fujishiro M, Tsutsumi Y, Ichinose M, Koike K.  Cathepsin E is a marker of gastric differentiation and signet-ring cell carcinoma of stomach: a novel suggestion on gastric tumorigenesis.  PLoS One  2013  8(2):e56766  PubMed ID: 23451082   DOI: 10.1371/journal.pone.0056766
15746  Hayakawa Y, Hirata Y, Sakitani K, Nakagawa H, Nakata W, Kinoshita H, Takahashi R, Takeda K, Ichijo H, Maeda S, Koike K.  Apoptosis signal-regulating kinase-1 inhibitor as a potent therapeutic drug for the treatment of gastric cancer  Cancer Sci  2012  103:2181-5  PubMed ID: 23110662   DOI: 10.1111/cas.12024
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
13836  Murai Y, Hayashi S, Takahashi H, Tsuneyama K, Takano Y.  Correlation between DNA alterations and p53 and p16 protein expression in cancer cell lines  Pathol Res Pract  2005  201(2):109-15  PubMed ID: 15901131   DOI: 10.1016/j.prp.2005.01.002
5952  Okada E, Murai Y, Matsui K, Isizawa S, Cheng C, Masuda M, Takano Y.  Survivin expression in tumor cell nuclei is predictive of a favorable prognosis in gastric cancer patients.  Cancer Lett  2001  163(1):109-16  PubMed ID: 11163114   DOI: 10.1016/s0304-3835(00)00677-7

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