Cell No. : Cell Name
RCB1179 : HuG1-N
update : 2025/04/22
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| Comment | Human cell line derived from ascites of a gastric cancer patient. Expressing Nagao-type alkaline phosphatase. HuG1-PI is another cell line derived from the same patient. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Imanishi, Hiroyasu
|
| Originator |
Imanishi, Hiroyasu
|
| Year of deposit |
1995
|
| Another name |
HuG
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
55 years
|
| Tissue |
stomach, ascites meta
|
| Disease name |
borrmann
|
| Metastatic ability |
Yes
|
| Metastatic tissue |
liver,peritoneum
|
| Classification |
cancer
|
| Year of origin |
1986
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_4846
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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RPMI1640 + 10% FBS
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
|
Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
|
| Isozyme |
|
LD, NP
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| Chromosome mode |
|
57-64(50) : /58(2),59(4),60(12),61(18),62(7),63(5)/
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| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
6
|
| User's Publication |
|
23331
Tedeschi A, Schischlik F, Rocchetti F, Popow J, Ebner F, Gerlach D, Geyer A, Santoro V, Boghossian AS, Rees MG, Ronan MM, Roth JA, Lipp J, Samwer M, Gmachl M, Kraut N, Pearson M, Rudolph D.
Pan-KRAS Inhibitors BI-2493 and BI-2865 Display Potent Antitumor Activity in Tumors with KRAS Wild-type Allele Amplification
Mol Cancer Ther
2025
24(4):550-562
PubMed ID: 39711431
DOI: 10.1158/1535-7163.MCT-24-0386
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5845
Konno-Shimizu M1, Yamamichi N, Inada K, Kageyama-Yahara N, Shiogama K, Takahashi Y, Asada-Hirayama I, Yamamichi-Nishina M, Nakayama C, Ono S, Kodashima S, Fujishiro M, Tsutsumi Y, Ichinose M, Koike K.
Cathepsin E is a marker of gastric differentiation and signet-ring cell carcinoma of stomach: a novel suggestion on gastric tumorigenesis.
PLoS One
2013
8(2):e56766
PubMed ID: 23451082
DOI: 10.1371/journal.pone.0056766
|
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15746
Hayakawa Y, Hirata Y, Sakitani K, Nakagawa H, Nakata W, Kinoshita H, Takahashi R, Takeda K, Ichijo H, Maeda S, Koike K.
Apoptosis signal-regulating kinase-1 inhibitor as a potent therapeutic drug for the treatment of gastric cancer
Cancer Sci
2012
103:2181-5
PubMed ID: 23110662
DOI: 10.1111/cas.12024
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6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
DOI: 10.1111/j.1749-0774.2009.00071.x
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13836
Murai Y, Hayashi S, Takahashi H, Tsuneyama K, Takano Y.
Correlation between DNA alterations and p53 and p16 protein expression in cancer cell lines
Pathol Res Pract
2005
201(2):109-15
PubMed ID: 15901131
DOI: 10.1016/j.prp.2005.01.002
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5952
Okada E, Murai Y, Matsui K, Isizawa S, Cheng C, Masuda M, Takano Y.
Survivin expression in tumor cell nuclei is predictive of a favorable prognosis in gastric cancer patients.
Cancer Lett
2001
163(1):109-16
PubMed ID: 11163114
DOI: 10.1016/s0304-3835(00)00677-7
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