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Cell No. : Cell Name
RCB1175 : GEM-199  update : 2022/03/29
CommentTumor cells derived from Gold Fish red pigment cells.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Matsumoto, Jiro
Originator Matsumoto, Jiro
Year of deposit 1995
Animal _gold fish < Fishes
Genus Carassius
Species auratus
Tissue body
Disease name erythrophoromas
Classification cancer
Year of origin 1978
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4204
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium L-15 + 20% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
2286  Matsumoto J, Ishikawa T, Masahito P, Takayama S  Permanent cell lines from erythrophoromas in goldfish (Carassius auratus).  J Natl Cancer Inst  1980  64:879-90  PubMed ID: 6928999  

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