Cell No. : Cell Name RCB1174 : GEM-81  update : 2024/09/12
Comment			Tumor cells derived from Gold Fish red pigment cells.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Matsumoto, Jiro
		Originator	Matsumoto, Jiro
		Year of deposit	1995
		Animal	_gold fish < Fishes
		Genus	Carassius
		Species	auratus
		Tissue	flank
		Disease name	erythrophoromas
		Classification	cancer
		Year of origin	1978
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_4206
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		L-15 + 20% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		25 ℃
		CO2 concentration		0 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	2

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Reference

2286  Matsumoto J, Ishikawa T, Masahito P, Takayama S  Permanent cell lines from erythrophoromas in goldfish (Carassius auratus).  J Natl Cancer Inst  1980  64:879-90  PubMed ID: 6928999

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User's Publication

21737  Shiomi A, Kaneko T, Nishikawa K, Tsuchida A, Isoshima T, Sato M, Toyooka K, Doi K, Nishikii H, Shintaku H.  High-throughput mechanical phenotyping and transcriptomics of single cells.  Nat Commun  2024  15(1):3812  PubMed ID: 38760380   DOI: 10.1038/s41467-024-48088-5

6272  Matsumoto J.  Brightly colored pigmentation in lower vertebrates: wonder searching its mechanisms and significance in the context of phylogeny.  Pigment Cell Res  2002  15(4):310-9  PubMed ID: 12100498   DOI: 10.1034/j.1600-0749.2002.02016.x