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Cell No. : Cell Name
RCB1171 : HOUFXXX  update : 2023/09/19
CommentStopped distribution. This cell line has been distributed as “Human fibroblasts derived from vagina. 47,XX,+X” until August 2023. It was proved that this cell line was a misidentified cell line of HOEF (RCB1010), human fibroblasts derived from endometrium with 47,XX,+X, deposited from the same scientist.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, Isamu
Year of deposit 1995
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 32 years
Tissue vagina
Disease name Trisomy X (47,XX,+X)
Classification normal
Year of origin 1995
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_E957
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 47(50)
STR(human) OK
Images
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lot info
Reference information Reference 0
User's Publication 0


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