Cell No. : Cell Name RCB1168 : SKW-3  update : 2024/08/14
Comment			Human cell line derived from acute lymphoblastic leukemia (ALL). A subline of KE-37. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 53 prior to order.
Comment from the depositor
Terms and conditions			1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 53 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	DSMZ
		Originator	Minowada, Jun
		Year of deposit	1995
		Another name	DSMZ no.: ACC 53
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Age at sampling	27 years
		Tissue	blood, T-cell leukemia
		Classification	cancer
		History	DSMZ (ACC53)
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_2197
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Virus (HIV)		(-)
		Virus (HTLV-1)		(-)
		Isozyme		LD, NP
		Chromosome mode		41-95(50) : 41(3),43(5),44(8),45(6),46(11),47(4)/
		STR(human)		OK
Reference information		Reference	1
		User's Publication	7

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Reference

1940  Hirano T, Kishimoto T, Kuritani T, Muraguchi A, Yamamura Y, Ralph P, Good, R A  In vitro immune response of human peripheral lymphocytes. IV. Specific induction of human suppressor T cells by an antiserum to the T leukemia cell line HSB.  J Immunol  1979  123:1133-40  PubMed ID: 313946

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User's Publication

21967  Okada M, Yamasaki S, Nakazato H, Hirahara Y, Ishibashi T, Kawamura M, Shimizu K, Fujii SI.  ARID1A-Deficient Tumors Acquire Immunogenic Neoantigens during the Development of Resistance to Targeted Therapy.  Cancer Res  2024  84(17):2792-2805  PubMed ID: 39228255   DOI: 10.1158/0008-5472.CAN-23-2846

21278  Okada M, Shimizu K, Nakazato H, Yamasaki S, Fujii SI.  Detection of mutant antigen-specific T cell receptors against multiple myeloma for T cell engineering.  Mol Ther Methods Clin Dev  2023  29:541-555  PubMed ID: 37359417   DOI: 10.1016/j.omtm.2023.05.014

19998  Yoshida K, Fujita A, Narazaki H, Asano T, Itoh Y.  Drug resistance to nelarabine in leukemia cell lines might be caused by reduced expression of deoxycytidine kinase through epigenetic mechanisms  Cancer Chemother Pharmacol  2022  89(1):83-91  PubMed ID: 34825941   DOI: 10.1007/s00280-021-04373-4

15878  Shimizu K, Iyoda T, Sanpei A, Nakazato H, Okada M, Ueda S, Kato-Murayama M, Murayama K, Shirouzu M, Harada N, Hidaka M, Fujii SI.  Identification of TCR repertoires in functionally competent cytotoxic T cells cross-reactive to SARS-CoV-2  Commun Biol   2021  4(1):1365  PubMed ID: 34857854   DOI: 10.1038/s42003-021-02885-6

13842  Hsieh HY, Jia W, Jin ZC, Kidoya H, Takakura N.  High expression of PSF1 promotes drug resistance and cell cycle transit in leukemia cells  Cancer Sci  2020  111(7):2400-2412  PubMed ID: 32391593   DOI: 10.1111/cas.14452

13994  Horlad H, Ma C, Yano H, Pan C, Ohnishi K, Fujiwara Y, Endo S, Kikukawa Y, Okuno Y, Matsuoka M, Takeya M, Komohara Y.  An IL-27/Stat3 axis induces expression of programmed cell death 1 ligands (PD-L1/2) on infiltrating macrophages in lymphoma  Cancer Sci  2016  107(11):1696-1704  PubMed ID: 27564404   DOI: 10.1111/cas.13065

6132  Naito H, Kidoya H, Sato Y, Takakura N.  Induction and expression of anti-angiogenic vasohibins in the hematopoietic stem/progenitor cell population.  J Biochem  2009  145(5):653-9  PubMed ID: 19179360   DOI: 10.1093/jb/mvp021