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Cell No. : Cell Name
RCB1168 : SKW-3  update : 2024/02/07
CommentHuman cell line derived from acute lymphoblastic leukemia (ALL). A subline of KE-37. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 53 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 53 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor DSMZ
Originator Minowada, Jun
Year of deposit 1995
Another name DSMZ no.: ACC 53
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 27 years
Tissue blood, T-cell leukemia
Classification cancer
History DSMZ (ACC53)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2197
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 41-95(50) : 41(3),43(5),44(8),45(6),46(11),47(4)/
STR(human) OK
Reference information Reference 1
User's Publication 6

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1940  Hirano T, Kishimoto T, Kuritani T, Muraguchi A, Yamamura Y, Ralph P, Good, R A  In vitro immune response of human peripheral lymphocytes. IV. Specific induction of human suppressor T cells by an antiserum to the T leukemia cell line HSB.  J Immunol  1979  123:1133-40  PubMed ID: 313946  

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User's Publication
21278  Okada M, Shimizu K, Nakazato H, Yamasaki S, Fujii SI.  Detection of mutant antigen-specific T cell receptors against multiple myeloma for T cell engineering.  Mol Ther Methods Clin Dev  2023  29:541-555  PubMed ID: 37359417   DOI: 10.1016/j.omtm.2023.05.014
19998  Yoshida K, Fujita A, Narazaki H, Asano T, Itoh Y.  Drug resistance to nelarabine in leukemia cell lines might be caused by reduced expression of deoxycytidine kinase through epigenetic mechanisms  Cancer Chemother Pharmacol  2022  89(1):83-91  PubMed ID: 34825941   DOI: 10.1007/s00280-021-04373-4
15878  Shimizu K, Iyoda T, Sanpei A, Nakazato H, Okada M, Ueda S, Kato-Murayama M, Murayama K, Shirouzu M, Harada N, Hidaka M, Fujii SI.  Identification of TCR repertoires in functionally competent cytotoxic T cells cross-reactive to SARS-CoV-2  Commun Biol   2021  4(1):1365  PubMed ID: 34857854   DOI: 10.1038/s42003-021-02885-6
13842  Hsieh HY, Jia W, Jin ZC, Kidoya H, Takakura N.  High expression of PSF1 promotes drug resistance and cell cycle transit in leukemia cells  Cancer Sci  2020  111(7):2400-2412  PubMed ID: 32391593   DOI: 10.1111/cas.14452
13994  Horlad H, Ma C, Yano H, Pan C, Ohnishi K, Fujiwara Y, Endo S, Kikukawa Y, Okuno Y, Matsuoka M, Takeya M, Komohara Y.  An IL-27/Stat3 axis induces expression of programmed cell death 1 ligands (PD-L1/2) on infiltrating macrophages in lymphoma  Cancer Sci  2016  107(11):1696-1704  PubMed ID: 27564404   DOI: 10.1111/cas.13065
6132  Naito H, Kidoya H, Sato Y, Takakura N.  Induction and expression of anti-angiogenic vasohibins in the hematopoietic stem/progenitor cell population.  J Biochem  2009  145(5):653-9  PubMed ID: 19179360   DOI: 10.1093/jb/mvp021

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