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Cell No. : Cell Name
RCB1167 : P-815  update : 2022/06/24
CommentMouse mastocytoma. Used as a target cells for cytotoxic T cell assays. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 1 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 1 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor DSMZ
Year of deposit 1995
Animal mouse < Mammals
Strain name DBA/2
Tissue mastocytoma
Classification cancer
History DSMZ (ACC1)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2154
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 6
User's Publication 7


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Reference
716  Ralph P, Nakoinz I  Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS.  J Immunol  1977  119:950-54  PubMed ID: 894031
1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890
1939  Ralph P, Nakoinz I  Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow orgin.  Nature  1974  249:49-51  PubMed ID: 4208429
1938  Ralph P, Nakoinz I, Raschke, W C  Lymphosarcoma cell growth is selectively inhibited by B lymphocyte mitogens: LPS, dextran sulfate and PPD.  Biochem Biophys Res Commun  1974  61:1268-75  PubMed ID: 4616699
4946  SCHELLENBERG H, DOMEN JOZ R.  P-815 mast cell tumor in suspension as a substrate for the testing of carcinostatic substances  Med Exp Int J Exp Med  1962  7:137-43  PubMed ID: 13991564
4945  DUNN TB, POTTER M.  A transplantable mast-cell neoplasm in the mouse.  J Natl Cancer Inst  1957  18(4):587-601  PubMed ID: 13416911

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User's Publication
14397  Takeda A, Tsubaki T, Sagae N, Onda Y, Inada Y, Mochizuki T, Okumura K, Kikuyama S, Kobayashi T, Iwamuro S.  Bacterial toxin-inducible gene expression of cathelicidin-B1 in the chicken bursal lymphoma-derived cell line DT40: functional characterization of cathelicidin-B1  Peptides  2014  59:94-10  PubMed ID: 24984089
16091  Masako Kawamoto and Kayo Masuko  Modulation of Mast Cell Function by Amino Acids In vitro: A Potential Mechanism of Immunonutrition for Wound Healing.  Journal of Nutritional Health & Food Science  2013  1 
13756  Murakami M, Kondo S, Funaba M.  Expression and function of alternative splice variants of the mouse TGF-beta type I receptor  Cell Biol Int  2008  32(7):848-54  PubMed ID: 18474439
2866  Sato, Katsuaki, Nakaoka, Takashi, Yamashita, Naohide, Yagita, Hideo, Kawasaki, Hiroshi, Morimoto, Chikao, Baba, Masanori, Matsuyama, Takami  TRAIL-transduced dendritic cells protect mice from acute graft-versus-host disease and leukemia relapse.  J Immunol  2005  174:4025-33  PubMed ID: 15778360
2668  Kurata M, Hirata M, Watabe S, Miyake M, Takahashi SY, Yamamoto Y  Expression, purification, and inhibitory activities of mouse cytotoxic T-lymphocyte antigen-2alpha.  Protein Expr Purif  2003  32:119-25  PubMed ID: 14680948
9038  Masahiro Nagahama, Shinya Hayashi, Shinsuke Morimitsu, Jun Sakurai  Biological Activities and Pore Formation of Clostridium Perfringens Beta Toxin in HL 60 Cells  J Biol Chem  2003  278 (38), 36934-41  PubMed ID: 12851396
5393  Harada M, Imai J, Okuno S, Suzuki T.  Macrophage-mediated activation of camptothecin analogue T-2513-carboxymethyl dextran conjugate (T-0128): possible cellular mechanism for antitumor activity.  J Control Release  2000  69(3):389-97  PubMed ID: 11102679



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