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Cell No. : Cell Name
RCB1166 : KG-1  update : 2021/02/26
CommentHuman acute myeloid leukemia. The variant subline (RCB1928 KG-1a) CD3-,CD13+,CD14-,CD15+,CD19-,CD33+. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 14 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 14 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor DSMZ
Year of deposit 1995
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 59 years
Tissue bone marrow
Disease name acute myeloid leukemia
Classification cancer
History DSMZ (ACC14)
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 25-119(50) : /41(2),43(7),44(3),45(3),46(6),47(1)/
deposit info
lot info
Reference information Reference 4
User's Publication 12

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2219  Konishi K, Tanabe F, Taniguchi M, Yamauchi H, Tanimoto T, Ikeda M, Orita K, Kurimoto M  A simple and sensitive bioassay for the detection of human interleukin-18/interferon-gamma-inducing factor using human myelomonocytic KG-1 cells.  J Immunol Methods  1997  209:187-91  PubMed ID: 9461334
880  Koeffler, H P  Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications.  Blood  1983  62:709-21  PubMed ID: 6192859
1937  Koeffler, H P, Golde, D W  Human myeloid leukemia cell lines: a review.  Blood  1980  56:344-50  PubMed ID: 6996765
625  Koeffler, H P, Golde, D W  Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity.  Science  1978  200:1153-4  PubMed ID: 306682

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User's Publication
11953  Tachibana K, Ohnishi H, Ali Haghparast SM, Kihara T, Miyake J.  Activation of PKC induces leukocyte adhesion by the dephosphorylation of ERM.  Biochem. Biophys. Res. Commun.  2020    PubMed ID: 31843195
12333  Tagod MSO, Mizuta S, Sakai Y, Iwasaki M, Shiraishi K, Senju H, Mukae H, Morita CT, Tanaka Y.  Determination of human γδ T cell-mediated cytotoxicity using a non-radioactive assay system  J Immunol Methods . 2019 Mar;466:32-40  2019  466:32-40  PubMed ID: 30654042
12516  Ono M, Takimoto R, Osuga T, Okagawa Y, Hirakawa M, Yoshida M, Arihara Y, Uemura N, Hayasaka N, Miura S, Matsuno T, Tamura F, Sato Y, Sato T, Iyama S, Miyanishi K, Takada K, Kobune M, Kato J.  Targeting Notch-1 positive acute leukemia cells by novel fucose-bound liposomes carrying daunorubicin  Oncotarget  2016  7(25):38586-38597  PubMed ID: 27233074
6082  Ohnishi H, Sasaki H, Nakamura Y, Kato S, Ando K, Narimatsu H, Tachibana K.  Regulation of cell shape and adhesion by CD34  Cell Adh Migr  2013  7(5):426-33  PubMed ID: 24036614
8778  Keiko Fukushima and Katsuko Yamashita  Carbohydrate Recognition by Cytokines and Its Relevance to Their Physiological Activities  Experimental Glycoscience  2008  154-157 
5983  Nemoto N, Suzukawa K, Shimizu S, Shinagawa A, Takei N, Taki T, Hayashi Y, Kojima H, Kawakami Y, Nagasawa T.  Identification of a novel fusion gene MLL-MAML2 in secondary acute myelogenous leukemia and myelodysplastic syndrome with inv(11)(q21q23).  Genes Chromosomes Cancer  2007  46(9):813-9  PubMed ID: 17551948
6144  Ninomiya M, Abe A, Katsumi A, Xu J, Ito M, Arai F, Suda T, Ito M, Kiyoi H, Kinoshita T, Naoe T.  Homing, proliferation and survival sites of human leukemia cells in vivo in immunodeficient mice.  Leukemia  2007  21(1):136-42  PubMed ID: 17039228
8818  Matsumoto E, Hatanaka M, Bohgaki M, Maeda S.  PKC pathway and ERK/MAPK pathway are required for induction of cyclin D1 and p21Waf1 during 12-o-tetradecanoylphorbol 13-acetate-induced differentiation of myeloleukemia cells.  Kobe J Med Sci  2006  52:181-94  PubMed ID: 17329956
3335  Yoshino K, Iimura E, Saijo K, Iwase S, Fukami K, Ohno T, Obata Y, Nakamura Y.  Essential role for gene profiling analysis in the authentication of human cell lines.  Hum Cell  2006  19(1):43-8  PubMed ID: 16643607
2974  Tamura H, Dan K, Tamada K, Nakamura K, Shioi Y, Hyodo H, Wang SD, Dong H, Chen L, Ogata K  Expression of functional B7-H2 and B7.2 costimulatory molecules and their prognostic implications in de novo acute myeloid leukemia  Clin Cancer Res  2005  15:5708-5717  PubMed ID: 16115907
2913  Fukushima K, Ikehara Y, Yamashita K  Functional role played by the glycosylphosphatidylinositol anchor glycan of CD48 in interleukin-18-induced interferon-gamma production.  J Biol Chem  2005  280:18056-62  PubMed ID: 15760905
5943  Rumi MA, Sato H, Ishihara S, Ortega C, Kadowaki Y, Kinoshita Y.  Growth inhibition of esophageal squamous carcinoma cells by peroxisome proliferator-activated receptor-gamma ligands.  J Lab Clin Med  2002  140(1):17-26  PubMed ID: 12080324

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