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Cell No. : Cell Name
RCB1164 : MOLT-3 update : 2025/01/16 |
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| Comment | Human T cell leukemia, the same patient as RCB0206 MOLT-4. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 84 prior to order. | |||
| Comment from the depositor | ||||
| Terms and conditions | 1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 84 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | DSMZ | ||
| Originator | Minowada, Jun | |||
| Year of deposit | 1995 | |||
| Animal | _human < Mammals | |||
| Genus | Homo | |||
| Species | sapiens | |||
| Gender | Male | |||
| Age at sampling | 19 years | |||
| Tissue | blood, T-cell leukemia | |||
| Classification | cancer | |||
| History | DSMZ (ACC84) | |||
| Lifespan | infinite | |||
| Morphology | lymphocyte-like | |||
| Cellosaurus(Expasy) | CVCL_0624 | |||
| Medium | Medium List | |||
| Culture type | Suspension cells | |||
| Culture method | General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | RPMI1640 + 10% FBS | |||
| Antibiotics | Free | |||
| Passage method | dilution | |||
| Culture information | Passage ratio | 1 : 10 split | ||
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Virus (HIV) | (-) | |||
| Virus (HTLV-1) | (-) | |||
| Isozyme | LD, NP | |||
| Chromosome mode | 94-100(50) : /95(5), 96(7), 97(15), 98(14), 99(5)/ | |||
| Surface antigen | CD3(-), CD4(+,low), CD8(+) | |||
| STR(human) | OK | |||
| Reference information | Reference | 7 | ||
| User's Publication | 3 | |||
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| Reference |
| 4748 Nakazawa S, Minowada J, Tsubota T, Sinks LF. Profound radiosensitivity in "leukemic" T-cell lines and T-cell-type acute lymphoblastic leukemia demonstrated by sodium [51Cr]chromate labeling. Cancer Res 1978 38(6):1661-6 PubMed ID: 306283 |
| 162 Ohnuma T, Holland JF, Arkin H, Minowada J. L-asparagine requirements of human T-lymphocytes and B-lymphocytes in culture J Natl Cancer Inst 1977 59(4):1061-3 PubMed ID: 302863 DOI: 10.1093/jnci/59.4.1061 |
| 164 Tsubota T, Minowada J, Nakazawa S, Sinks LF, Han T, Higby RJ, Pressman D. Correlation of surface markers of cells of human lymphatic leukemias with disease type J Natl Cancer Inst 1977 59(3):845-50 PubMed ID: 302342 DOI: 10.1093/jnci/59.3.845 |
| 160 Hayata I, Oshimura M, Minowada J, Sandberg AA. Chromosomal banding of cultured T and B lymphocytes In Vitro 1975 11(6):361-8. PubMed ID: 1081484 DOI: 10.1007/BF02616372 |
| 163 Kataoka K, Minowada J, Pressman D. Electron microscope study on human lymphocyte-sheep erythrocyte rosettes J Natl Cancer Inst 1975 55(6):1323-5 PubMed ID: 1239515 DOI: 10.1093/jnci/55.6.1323 |
| 165 Srivastava BI, Minowada J, Moore GE. High terminal deoxynucleotidyl transferase activity in a new T-cell line (RPMI 8402) of acute lymphoblastic leukemia origin J Natl Cancer Inst 1975 55(1):11-14 PubMed ID: 808634 DOI: 10.1093/jnci/55.1.11 |
| 494 Minowada J, Onuma T, Moore, G E Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J Natl Cancer Inst 1972 49:891-5 PubMed ID: 4567231 |
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| User's Publication |
| 7587 Ogawa A, Akatsuka R, Tamauchi H, Hio K, Kanematsu H. Influence of the nickel-titanium alloy components on biological functions. BMC Proc 2011 5 Suppl 8:P79 PubMed ID: 22373219 DOI: 10.1186/1753-6561-5-S8-P79 |
| 8914 Hisakage Funabashi, Riko Matsuzawaa, Makiko Nakamura, Masayasu Mie and Eiry Kobatake Bioluminescent enumeration of surface antigen-specific cells using the streptavidin?luciferase fusion protein Sensors and Actuators B: Chemical 2006 120:51-56 DOI: 10.1016/j.snb.2006.01.042 |
| 15089 Kuraya M, Matsushita M, Endo Y, Thiel S, Fujita T. Expression of H-ficolin/Hakata antigen, mannose-binding lectin-associated serine protease (MASP)-1 and MASP-3 by human glioma cell line T98G Int Immunol 2003 15(1):109-17 PubMed ID: 12502731 DOI: 10.1093/intimm/dxg008 |
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