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Cell No. : Cell Name
RCB1164 : MOLT-3  update : 2024/01/17
CommentHuman T cell leukemia, the same patient as RCB0206 MOLT-4. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 84 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 84 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor DSMZ
Originator Minowada, Jun
Year of deposit 1995
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 19 years
Tissue blood, T-cell leukemia
Classification cancer
History DSMZ (ACC84)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0624
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 94-100(50) : /95(5), 96(7), 97(15), 98(14), 99(5)/
Surface antigen CD3(-), CD4(+,low), CD8(+)
STR(human) OK
Reference information Reference 7
User's Publication 3


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Reference
4748  Nakazawa S, Minowada J, Tsubota T, Sinks LF.  Profound radiosensitivity in "leukemic" T-cell lines and T-cell-type acute lymphoblastic leukemia demonstrated by sodium [51Cr]chromate labeling.  Cancer Res  1978  38(6):1661-6  PubMed ID: 306283  
162  Ohnuma T, Holland JF, Arkin H, Minowada J.  L-asparagine requirements of human T-lymphocytes and B-lymphocytes in culture  J Natl Cancer Inst  1977  59(4):1061-3  PubMed ID: 302863   DOI: 10.1093/jnci/59.4.1061
164  Tsubota T, Minowada J, Nakazawa S, Sinks LF, Han T, Higby RJ, Pressman D.  Correlation of surface markers of cells of human lymphatic leukemias with disease type  J Natl Cancer Inst  1977  59(3):845-50  PubMed ID: 302342   DOI: 10.1093/jnci/59.3.845
160  Hayata I, Oshimura M, Minowada J, Sandberg AA.  Chromosomal banding of cultured T and B lymphocytes  In Vitro  1975  11(6):361-8.  PubMed ID: 1081484   DOI: 10.1007/BF02616372
163  Kataoka K, Minowada J, Pressman D.  Electron microscope study on human lymphocyte-sheep erythrocyte rosettes  J Natl Cancer Inst  1975  55(6):1323-5  PubMed ID: 1239515   DOI: 10.1093/jnci/55.6.1323
165  Srivastava BI, Minowada J, Moore GE.  High terminal deoxynucleotidyl transferase activity in a new T-cell line (RPMI 8402) of acute lymphoblastic leukemia origin  J Natl Cancer Inst  1975  55(1):11-14  PubMed ID: 808634   DOI: 10.1093/jnci/55.1.11
494  Minowada J, Onuma T, Moore, G E  Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes.  J Natl Cancer Inst  1972  49:891-5  PubMed ID: 4567231  

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User's Publication
7587  Ogawa A, Akatsuka R, Tamauchi H, Hio K, Kanematsu H.  Influence of the nickel-titanium alloy components on biological functions.  BMC Proc  2011  5 Suppl 8:P79  PubMed ID: 22373219   DOI: 10.1186/1753-6561-5-S8-P79
8914  Hisakage Funabashi, Riko Matsuzawaa, Makiko Nakamura, Masayasu Mie and Eiry Kobatake  Bioluminescent enumeration of surface antigen-specific cells using the streptavidin?luciferase fusion protein  Sensors and Actuators B: Chemical  2006  120:51-56    DOI: 10.1016/j.snb.2006.01.042
15089  Kuraya M, Matsushita M, Endo Y, Thiel S, Fujita T.  Expression of H-ficolin/Hakata antigen, mannose-binding lectin-associated serine protease (MASP)-1 and MASP-3 by human glioma cell line T98G  Int Immunol  2003  15(1):109-17  PubMed ID: 12502731   DOI: 10.1093/intimm/dxg008


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