Cell No. : Cell Name RCB1164 : MOLT-3  update : 2025/01/16
Comment			Human T cell leukemia, the same patient as RCB0206 MOLT-4. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 84 prior to order.
Comment from the depositor
Terms and conditions			1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 84 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	DSMZ
		Originator	Minowada, Jun
		Year of deposit	1995
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Age at sampling	19 years
		Tissue	blood, T-cell leukemia
		Classification	cancer
		History	DSMZ (ACC84)
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_0624
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Virus (HIV)		(-)
		Virus (HTLV-1)		(-)
		Isozyme		LD, NP
		Chromosome mode		94-100(50) : /95(5), 96(7), 97(15), 98(14), 99(5)/
		Surface antigen		CD3(-), CD4(+,low), CD8(+)
		STR(human)		OK
Reference information		Reference	7
		User's Publication	3

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Reference

4748  Nakazawa S, Minowada J, Tsubota T, Sinks LF.  Profound radiosensitivity in "leukemic" T-cell lines and T-cell-type acute lymphoblastic leukemia demonstrated by sodium [51Cr]chromate labeling.  Cancer Res  1978  38(6):1661-6  PubMed ID: 306283

162  Ohnuma T, Holland JF, Arkin H, Minowada J.  L-asparagine requirements of human T-lymphocytes and B-lymphocytes in culture  J Natl Cancer Inst  1977  59(4):1061-3  PubMed ID: 302863   DOI: 10.1093/jnci/59.4.1061

164  Tsubota T, Minowada J, Nakazawa S, Sinks LF, Han T, Higby RJ, Pressman D.  Correlation of surface markers of cells of human lymphatic leukemias with disease type  J Natl Cancer Inst  1977  59(3):845-50  PubMed ID: 302342   DOI: 10.1093/jnci/59.3.845

160  Hayata I, Oshimura M, Minowada J, Sandberg AA.  Chromosomal banding of cultured T and B lymphocytes  In Vitro  1975  11(6):361-8.  PubMed ID: 1081484   DOI: 10.1007/BF02616372

163  Kataoka K, Minowada J, Pressman D.  Electron microscope study on human lymphocyte-sheep erythrocyte rosettes  J Natl Cancer Inst  1975  55(6):1323-5  PubMed ID: 1239515   DOI: 10.1093/jnci/55.6.1323

165  Srivastava BI, Minowada J, Moore GE.  High terminal deoxynucleotidyl transferase activity in a new T-cell line (RPMI 8402) of acute lymphoblastic leukemia origin  J Natl Cancer Inst  1975  55(1):11-14  PubMed ID: 808634   DOI: 10.1093/jnci/55.1.11

494  Minowada J, Onuma T, Moore, G E  Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes.  J Natl Cancer Inst  1972  49:891-5  PubMed ID: 4567231

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User's Publication

7587  Ogawa A, Akatsuka R, Tamauchi H, Hio K, Kanematsu H.  Influence of the nickel-titanium alloy components on biological functions.  BMC Proc  2011  5 Suppl 8:P79  PubMed ID: 22373219   DOI: 10.1186/1753-6561-5-S8-P79

8914  Hisakage Funabashi, Riko Matsuzawaa, Makiko Nakamura, Masayasu Mie and Eiry Kobatake  Bioluminescent enumeration of surface antigen-specific cells using the streptavidin?luciferase fusion protein  Sensors and Actuators B: Chemical  2006  120:51-56    DOI: 10.1016/j.snb.2006.01.042

15089  Kuraya M, Matsushita M, Endo Y, Thiel S, Fujita T.  Expression of H-ficolin/Hakata antigen, mannose-binding lectin-associated serine protease (MASP)-1 and MASP-3 by human glioma cell line T98G  Int Immunol  2003  15(1):109-17  PubMed ID: 12502731   DOI: 10.1093/intimm/dxg008