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Cell No. : Cell Name
RCB1158 : B16F10/mGM  update : 2022/07/20
CommentMouse GM-CSF producing B16 melanoma.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hamada, Hirofumi
Originator Hamada, Hirofumi
Year of deposit 1995
Another name B16F10/mGM-CSF
Animal _mouse < Mammals
Strain name C57BL/6J
Tissue skin
Disease name melanoma
Classification cancer
Recombinant recombinant
Exogene MFG-mGM-CSF
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_L292
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 0
User's Publication 5

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User's Publication
21194  Fukaya T, Uto T, Mitoma S, Takagi H, Nishikawa Y, Tominaga M, Choijookhuu N, Hishikawa Y, Sato K.  Gut dysbiosis promotes the breakdown of oral tolerance mediated through dysfunction of mucosal dendritic cells.  Cell Rep  2023  42(5):112431  PubMed ID: 37099426   DOI: 10.1016/j.celrep.2023.112431
12087  Minh Nguyen H, Truong Nguyen H, Win N, Piow Wong C, Vu Huynh KL, Hoang NN, Do KM, Thi Nguyen H, Viet Ho D, Duc Nguyen M, Morita H.  Antimelanogenic Activity of Ocotillol-Type Saponins from Panax vietnamensis.  Chem. Biodivers.  2020    PubMed ID: 32163220   DOI: 10.1002/cbdv.202000037
4159  Win NN, Woo SY, Ngwe H, Prema, Wong CP, Ito T, Okamoto Y, Tanaka M, Imagawa H, Asakawa Y, Abe I, Morita H.  Tetrahydrofuran lignans: Melanogenesis inhibitors from Premna integrifolia wood collected in Myanmar.  Fitoterapia  2018    PubMed ID: 29540314   DOI: 10.1016/j.fitote.2018.03.002
9840  Sato K, Ando R, Kobayashi H, Nishio T.  2-Ethoxybenzamide stimulates melanin synthesis in B16F1 melanoma cells via the CREB signaling pathway.  Mol. Cell. Biochem.  2016  423:39-52  PubMed ID: 27633503   DOI: 10.1007/s11010-016-2823-x
14583  Kondo T, Tsunematsu T, Yamada A, Arakaki R, Saito M, Otsuka K, Kujiraoka S, Ushio A, Kurosawa M, Kudo Y, Ishimaru N.  Acceleration of tumor growth due to dysfunction in M1 macrophages and enhanced angiogenesis in an animal model of autoimmune disease  Lab Invest  2016  96(4):468-80  PubMed ID: 26808709   DOI: 10.1038/labinvest.2015.166

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