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Cell No. : Cell Name
RCB1157 : UV-P3U1-B8  update : 2022/03/29
CommentAnti-thymine dimer antibody producing. Serum-free cultured hybridoma.
Comment from the depositor
Terms and conditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Tamaki, Yoshihiro
Originator Tamaki, Yoshihiro
Year of deposit 1995
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Year of origin 1994
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U860
hybridoma Fusion_cell_1 name lymph node
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_1 gender Unknown
Fusion_cell_2 name P3-X63-Ag8-U1
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name anti T-T dimer
Subclass IgG3
Antigen_name T-T dimer
Antigen_epitope T-T dimer
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives ASF103
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium ASF103 : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG3
Reference information Reference 1
User's Publication 1


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Reference
1975  Tamaki Y, Kishida T  Efficient methods for production of monoclonal antibodies for forensic applications.  Nippon Hoigaku Zasshi  1992  46:424-7  PubMed ID: 1303446  

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User's Publication
16791  Miyoshi T, Friedman TB, Watanabe N.  Fast-dissociating but highly specific antibodies are novel tools in biology, especially useful for multiplex super-resolution microscopy  STAR Protoc  2021  2(4):100967  PubMed ID: 34841279   DOI: 10.1016/j.xpro.2021.100967



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