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Cell No. : Cell Name
RCB1155 : Hepa-E1  update : 2020/09/24
CommentEel hepatocyte. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Kong Zwe-Ling
Year of deposit 1995
Animal eel < Fishes
Tissue liver
Classification other
History overseas
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives E-RDF + 5% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4-6 split
SC frequency Subculture : 2 times/week
Temperature 28 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Images
deposit info
lot info
Reference information Reference 0
User's Publication 3


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Reference

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User's Publication
9912  Hiroshi Suzuki, Yuichi Ozaki, Shigeho Ijiri, Koichiro Gen, Yukinori Kazeto  17β-Hydroxysteroid Dehydrogenase Type 12a Responsible for Testicular 11-ketotestosterone Synthesis in the Japanese Eel, Anguilla Japonica  J Steroid Biochem Mol Biol  2020  198:105550  PubMed ID: 31778803
11574  Franco ME, Hill BN, Brooks BW, Lavado R.  Prymnesium parvum differentially triggers sublethal fish antioxidant responses in vitro among salinity and nutrient conditions.  Aquat. Toxicol.  2019    PubMed ID: 31185429
9477  Kotowa Sakai, Yuya Yamamoto 1, Toshitaka Ikeuchi 2  Vertebrates Originally Possess Four Functional Subtypes of G Protein-Coupled Melatonin Recepto  Sci Rep  2019  9(1):9465  PubMed ID: 31263128



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