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Cell No. : Cell Name
RCB1154 : KGN  update : 2021/02/26
CommentHuman cell line derived from ovarian granulosa cell tumor. Aromatase activity is increased by stimulation of FSH and hMG.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nishi, Y. & Nawata, H.
Originator Nishi, Yoshihiro & Nawata, Hajime
Year of deposit 1995
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 69 years
Tissue ovary, peritoneum
Disease name granulosa cell tumor
Classification cancer
Year of origin 1993
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 43-46(50) : 43(1),44(1),45(45),46(3)
deposit info
lot info
Reference information Reference 10
User's Publication 54

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4938  Bao B, Jiang J, Yanase T, Nishi Y, Morgan JR.  Connexon-mediated cell adhesion drives microtissue self-assembly.  FASEB J  2011  25(1):255-64  PubMed ID: 20876208
4936  Kwintkiewicz J, Nishi Y, Yanase T, Giudice LC.  Peroxisome proliferator-activated receptor-gamma mediates bisphenol A inhibition of FSH-stimulated IGF-1, aromatase, and estradiol in human granulosa cells.  Environ Health Perspect  2010  118(3):400-6  PubMed ID: 20064783
4935  Jääskeläinen M, Kyrönlahti A, Anttonen M, Nishi Y, Yanase T, Secchiero P, Zauli G, Tapanainen JS, Heikinheimo M, Vaskivuo TE.  TRAIL pathway components and their putative role in granulosa cell apoptosis in the human ovary.  Differentiation  2009  77(4):369-76  PubMed ID: 19281785
4934  Mottershead DG1, Pulkki MM, Muggalla P, Pasternack A, Tolonen M, Myllymaa S, Korchynskyi O, Nishi Y, Yanase T, Lun S, Juengel JL, Laitinen M, Ritvos O.  Characterization of recombinant human growth differentiation factor-9 signaling in ovarian granulosa cells.  Mol Cell Endocrinol  2008  283(1-2):58-67  PubMed ID: 18162287
4933  Wu Y, Ghosh S, Nishi Y, Yanase T, Nawata H, Hu Y.  The orphan nuclear receptors NURR1 and NGFI-B modulate aromatase gene expression in ovarian granulosa cells: a possible mechanism for repression of aromatase expression upon luteinizing hormone surge.  Endocrinology  2005  146(1):237-46  PubMed ID: 15486232
4932  Chu S, Nishi Y, Yanase T, Nawata H, Fuller PJ.  Transrepression of estrogen receptor beta signaling by nuclear factor-kappab in ovarian granulosa cells.  Mol Endocrinol  2004  18(8):1919-28  PubMed ID: 15155785
4930  Fujii A, Harada T, Yamauchi N, Iwabe T, Nishi Y, Yanase T, Nawata H, Terakawa N.  Interleukin-8 gene and protein expression are up-regulated by interleukin-1beta in normal human ovarian cells and a granulosa tumor cell line.  Fertil Steril  2003  79(1):151-7  PubMed ID: 12524080
4931  Mukasa C, Nomura M, Tanaka T, Tanaka K, Nishi Y, Okabe T, Goto K, Yanase T, Nawata H.  Activin signaling through type IB activin receptor stimulates aromatase activity in the ovarian granulosa cell-like human granulosa (KGN) cells.  Endocrinology  2003  144(4):1603-11  PubMed ID: 12639945
4928  Nishi Y, Yanase T, Mu Y, Oba K, Ichino I, Saito M, Nomura M, Mukasa C, Okabe T, Goto K, Takayanagi R, Kashimura Y, Haji M, Nawata H.  Establishment and characterization of a steroidogenic human granulosa-like tumor cell line, KGN, that expresses functional follicle-stimulating hormone receptor.  Endocrinology  2001  42(1):437-45  PubMed ID: 11145608
4929  Saitoh M, Yanase T, Morinaga H, Tanabe M, Mu YM, Nishi Y, Nomura M, Okabe T, Goto K, Takayanagi R, Nawata H.  Tributyltin or triphenyltin inhibits aromatase activity in the human granulosa-like tumor cell line KGN.  Biochem Biophys Res Commun  2001  289(1):198-204  PubMed ID: 11708799

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User's Publication
13389  Li D, Wang X, Dang Y, Zhang X, Zhao S, Lu G, Chan WY, Leung PCK, Qin Y.  lncRNA GCAT1 is involved in premature ovarian insufficiency by regulating p27 translation in GCs via competitive binding to PTBP1.  Mol Ther Nucleic Acids  2021    PubMed ID: 33335798
11980  Gogola J, Hoffmann M, Nimpsz S, Ptak A.  Disruption of 17β-estradiol secretion by persistent organic pollutants present in human follicular fluid is dependent on the potential of ovarian granulosa tumor cell lines to metabolize estrogen.  Mol. Cell. Endocrinol.  2020    PubMed ID: 31891770
11988  Gogola J, Hoffmann M, Ptak A.  Persistent endocrine-disrupting chemicals found in human follicular fluid stimulate IGF1 secretion by adult ovarian granulosa cell tumor spheroids and thereby increase proliferation of non-cancer ovarian granulosa cells.  Toxicol In Vitro  2020    PubMed ID: 31923581
12055  Wu G, Yang Z, Chen Y, Li X, Yang J, Yin T.  Downregulation of Lnc-OC1 attenuates the pathogenesis of polycystic ovary syndrome.  Mol. Cell. Endocrinol.  2020    PubMed ID: 32070768
12068  Wang X, Zhang X, Dang Y, Li D, Lu G, Chan WY, Leung PCK, Zhao S, Qin Y, Chen ZJ.  Long noncoding RNA HCP5 participates in premature ovarian insufficiency by transcriptionally regulating MSH5 and DNA damage repair via YB1.  Nucleic Acids Res.  2020    PubMed ID: 32112110
12093  Nishigaki A, Kido T, Kida N, Kakita-Kobayashi M, Tsubokura H, Hisamatsu Y, Okada H.  Resveratrol protects mitochondrial quantity by activating SIRT1/PGC-1α expression during ovarian hypoxia.  Reprod. Med. Biol.  2020    PubMed ID: 32273826
12161  Xiangmin Lv, Chunbo He, Cong Huang , Guohua Hua, Xingcheng Chen, Barbara K.Timm, Victoria M.Maclin, Abigail A.Haggerty, Shelly K.Aust, Denae M.Golden, Bhavana J.Dave, Yun-An Tseng, Li Chen, Hongbo Wang, Peichao Chen, David L.Klinkebiel, Adam R.Karpf, Jixin Dong, Ronny I.Drapkin, Bo R.Rueda, John S.Davis, Cheng Wang  Reprogramming of ovarian granulosa cells by YAP1 leads to development of high-grade cancer with mesenchymal lineage and serous features  Science Bulletin  2020   
12495  Silber M, Miller I, Bar-Joseph H, Ben-Ami I, Shalgi R.  Elucidating the role of pigment epithelium-derived factor (PEDF) in metabolic PCOS models  J Endocrinol  2020  244(2):297-308  PubMed ID: 31661672
12995  Jin H, Lee B, Luo Y, Choi Y, Choi EH, Jin H, Kim KB, Seo SB, Kim YH, Lee HH, Kim KP, Lee K, Bae J.  FOXL2 directs DNA double-strand break repair pathways by differentially interacting with Ku.  Nat Commun  2020    PubMed ID: 32332759
13030  Zhang X, Dang Y, Liu R, Zhao S, Ma J, Qin Y.  MicroRNA-127-5p impairs function of granulosa cells via HMGB2 gene in premature ovarian insufficiency.  J Cell Physiol  2020    PubMed ID: 32391592
13187  Lin YY, Sun D, Wu YL.  Novel regulation of PKC-induced inflammation by Akt and protein phosphatase 2A in ovarian granulosa cells.  Chin J Physiol  2020    PubMed ID: 32859885
13507  Lee SY, Kang YJ, Kwon J, Nishi Y, Yanase T, Lee KA, Koong MK.  miR-4463 regulates aromatase expression and activity for 17β-estradiol synthesis in response to follicle-stimulating hormone  Clin Exp Reprod Med  2020  47(3):194-206  PubMed ID: 32854459
11293  Gogola J, Hoffmann M, Ptak A.  Persistent endocrine-disrupting chemicals found in human follicular fluid stimulate the proliferation of granulosa tumor spheroids via GPR30 and IGF1R but not via the classic estrogen receptors.  Chemosphere  2019    PubMed ID: 30414542
11466  Buck T, Hack CT, Berg D, Berg U, Kunz L, Mayerhofer A.  The NADPH oxidase 4 is a major source of hydrogen peroxide in human granulosa-lutein and granulosa tumor cells.  Sci Rep  2019    PubMed ID: 30837663
11481  Li Y, Xiang Y, Song Y, Wan L, Yu G, Tan L.  Dysregulated miR-142, -33b, and -423 in granulosa cells target TGFBR1 and SMAD7: a possible role in polycystic ovary syndrome.  Mol. Hum. Reprod.  2019    PubMed ID: 30865275
11596  Lv X, He C, Huang C, Wang H, Hua G, Wang Z, Zhou J, Chen X, Ma B, Timm BK, Maclin V, Dong J, Rueda BR, Davis JS, Wang C.  Timely expression and activation of YAP1 in granulosa cells is essential for ovarian follicle development.  FASEB J.  2019    PubMed ID: 31199671
11734  Lin PH, Chang CC, Wu KH, Shih CK, Chiang W, Chen HY, Shih YH, Wang KL, Hong YH, Shieh TM, Hsia SM.  Dietary Glycotoxins, Advanced Glycation End Products, Inhibit Cell Proliferation and Progesterone Secretion in Ovarian Granulosa Cells and Mimic PCOS-like Symptoms.  Biomolecules  2019    PubMed ID: 31370285
11752  Li Y, Liu YD, Zhou XY, Chen SL, Chen X, Zhe J, Zhang J, Zhang QY, Chen YX.  MiR-29a regulates the proliferation, aromatase expression, and estradiol biosynthesis of human granulosa cells in polycystic ovary syndrome.  Mol. Cell. Endocrinol.  2019    PubMed ID: 31421163
11753  Bagnjuk K, Kast VJ, Tiefenbacher A, Kaseder M, Yanase T, Burges A, Kunz L, Mayr D, Mayerhofer A.  Inhibitor of apoptosis proteins are potential targets for treatment of granulosa cell tumors - implications from studies in KGN.  J Ovarian Res  2019    PubMed ID: 31412918
11754  He T, Sun Y, Zhang Y, Zhao S, Zheng Y, Hao G, Shi Y.  MicroRNA-200b and microRNA-200c are up-regulated in PCOS granulosa cell and inhibit KGN cell proliferation via targeting PTEN.  Reprod. Biol. Endocrinol.  2019    PubMed ID: 31421682
11876  Hack CT, Buck T, Bagnjuk K, Eubler K, Kunz L, Mayr D, Mayerhofer A.  A Role for H2O2 and TRPM2 in the Induction of Cell Death: Studies in KGN Cells.  Antioxidants (Basel)  2019    PubMed ID: 31671815
11961  Jung DH, Hwang JT, Pyun BJ, Yu SY, Ko BS.  Assessment of the Aromatase Inhibitory Activity of Ma-Huang-Tang (MHT) and Its Active Compounds.  Evid Based Complement Alternat Med  2019    PubMed ID: 31929813
12493  Li Y, Zheng Q, Sun D, Cui X, Chen S, Bulbul A, Liu S, Yan Q.  Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome  J Cell Physiol  2019  234(5):7435-7447  PubMed ID: 30580448
12494  Huang X, She L, Luo X, Huang S, Wu J.  MiR-222 promotes the progression of polycystic ovary syndrome by targeting p27 Kip1  Pathol Res Pract  2019  215(5):918-923.  PubMed ID: 30718101
12496  Liu Y, Wang Y, Wang F, Pan J, Xu J, Li J, Zhou C, Ding G, Wu Y, Liu X, Sheng J, Huang H.  Mechanism underlying the retarded nuclear translocation of androgen receptor splice variants  Sci China Life Sci  2019  62(2):257-267  PubMed ID: 30267260
10723  Dang Y, Wang X, Hao Y, Zhang X, Zhao S, Ma J, Qin Y, Chen ZJ.  MicroRNA-379-5p is associate with biochemical premature ovarian insufficiency through PARP1 and XRCC6.  Cell Death Dis  2018  9:106  PubMed ID: 29367615
10810  Hoffmann M, Rak A, Ptak A.  Bisphenol A and its derivatives decrease expression of chemerin, which reverses its stimulatory action in ovarian cancer cells.  Toxicol. Lett.  2018  291:61-69  PubMed ID: 29653259
11053  Leila Bouazzi, Mariangela Franco, Alexander Maret and Anna Biason-Lauber  CBX2 and the Ovary: Novel Insights into Regulatory Networks in Humans  J Gynecol Reprod Med  2018  2:1-9 
11114  Hillman RT, Celestino J, Terranova C, Beird HC, Gumbs C, Little L, Nguyen T, Thornton R, Tippen S, Zhang J, Lu KH, Gershenson DM, Rai K, Broaddus RR, Futreal PA.  KMT2D/MLL2 inactivation is associated with recurrence in adult-type granulosa cell tumors of the ovary.  Nat Commun  2018  9(1):2496  PubMed ID: 29950560
4273  Hao J, Tuck AR, Sjödin MOD, Lindberg J, Sand A, Niklasson B, Argyraki M, Hovatta O, Damdimopoulou P.  Resveratrol supports and alpha-naphthoflavone disrupts growth of human ovarian follicles in an in vitro tissue culture model.  Toxicol. Appl. Pharmacol.  2018    PubMed ID: 29146461
9709  Hirano M, Wada-Hiraike O, Fu H, Akino N, Isono W, Sakurabashi A, Fukuda T, Morita Y, Tanikawa M, Miyamoto Y, Nishi Y, Yanase T, Harada M, Oishi H, Yano T, Koga K, Oda K, Kawana K, Fujii T, Osuga Y.  The Emerging Role of FOXL2 in Regulating the Transcriptional Activation Function of Estrogen Receptor β: An Insight Into Ovarian Folliculogenesis.  Reprod Sci  2017  24(1):133-141  PubMed ID: 27252187
10083  Hoffmann M, Fiedor E, Ptak A.  Bisphenol A and its derivatives tetrabromobisphenol A and tetrachlorobisphenol A induce apelin expression and secretion in ovarian cancer cells through a peroxisome proliferator-activated receptor gamma-dependent mechanism.  Toxicol. Lett.  2017  269:15-22  PubMed ID: 28111160
10221  Colombe S, Houllier L, Fleurot E, Levallet G, Benhaïm A, Bonnamy PJ, Levallet J.  Syndecan 1 represses cell growth and FSH responsiveness in human granulosa cells.  Reproduction  2017  153:797-808  PubMed ID: 28348070
10394  Ma X, Hayes E, Biswas A, Seger C, Prizant H, Hammes SR, Sen A.  Androgens Regulate Ovarian Gene Expression Through Modulation of Ezh2 Expression and Activity.  Endocrinology  2017  158:2944-2954  PubMed ID: 28666321
10465  Hoffmann M, Gogola J, Kotula-Balak M, Ptak A.  Stimulation of ovarian cell proliferation by tetrabromobisphenol A but not tetrachlorobisphenol A through G protein-coupled receptor 30.  Toxicol In Vitro  2017  45:54-59  PubMed ID: 28811233
7361  Diamanti-Kandarakis E, Chatzigeorgiou A, Papageorgiou E, Koundouras D, Koutsilieris M.  Advanced glycation end-products and insulin signaling in granulosa cells.  Exp. Biol. Med. (Maywood)  2016  241:1438-45  PubMed ID: 25956684
9513  Ernst J, Grabiec U, Greither T, Fischer B, Dehghani F.  The endocannabinoid system in the human granulosa cell line KGN.  Mol. Cell. Endocrinol.  2016  423:67-76  PubMed ID: 26773729
9719  Dou YD, Zhao H, Huang T, Zhao SG, Liu XM, Yu XC, Ma ZX, Zhang YC, Liu T, Gao X, Li L, Lu G, Chan WY, Gao F, Liu HB, Chen ZJ.  STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells.  Sci Rep  2016  6:26691  PubMed ID: 27270953
9805  Chao YN, Sun D, Peng YC, Wu YL.  Growth Hormone Releasing Peptide-2 Attenuation of Protein Kinase C-Induced Inflammation in Human Ovarian Granulosa Cells.  Int J Mol Sci  2016  17:  PubMed ID: 27548147
10879  Hua G, He C, Lv X, Fan L, Wang C, Remmenga SW, Rodabaugh KJ, Yang L, Lele SM, Yang P, Karpf AR, Davis JS, Wang C.  The four and a half LIM domains 2 (FHL2) regulates ovarian granulosa cell tumor progression via controlling AKT1 transcription  Cell Death Dis  2016  7:e2297  PubMed ID: 27415427
12654  Huang X, Liu C, Hao C, Tang Q, Liu R, Lin S, Zhang L, Yan W.  Identification of altered microRNAs and mRNAs in the cumulus cells of PCOS patients: miRNA-509-3p promotes oestradiol secretion by targeting MAP3K8  Reproduction  2016  151(6):643-55  PubMed ID: 27001999
12972  Fang Y, Lu X, Liu L, Lin X, Sun M, Fu J, Xu S, Tan Y.  Vascular endothelial growth factor induces anti‑Müllerian hormone receptor 2 overexpression in ovarian granulosa cells of in vitro fertilization/intracytoplasmic sperm injection patients  Mol Med Rep  2016  13(6):5157-62.  PubMed ID: 27109000
7370  Jiang C, Hou X, Wang C, May JV, Butnev VY, Bousfield GR, Davis JS.  Hypoglycosylated hFSH Has Greater Bioactivity Than Fully Glycosylated Recombinant hFSH in Human Granulosa Cells.  J. Clin. Endocrinol. Metab.  2015  100:E852-60  PubMed ID: 25915568
7417  Francois CM, Wargnier R, Petit F, Goulvent T, Rimokh R, Treilleux I, Ray-Coquard I, Zazzu V, Cohen-Tannoudji J, Guigon CJ.  17β-estradiol inhibits spreading of metastatic cells from granulosa cell tumors through a non-genomic mechanism involving GPER1.  Carcinogenesis  2015  36:564-73  PubMed ID: 25823895
7428  He C, Lv X, Hua G, Lele SM, Remmenga S, Dong J, Davis JS, Wang C.  YAP forms autocrine loops with the ERBB pathway to regulate ovarian cancer initiation and progression.  Oncogene  2015  34:6040-54  PubMed ID: 25798835
10297  Jana Ernst, Johann-Christoph Jann, Ronald Biemann, Holger M Koch, Bernd Fischer  Effects of the Environmental Contaminants DEHP and TCDD on Estradiol Synthesis and Aryl Hydrocarbon Receptor and Peroxisome Proliferator-Activated Receptor Signalling in the Human Granulosa Cell Line KGN  Mol Hum Reprod  2014  20(9):919-28  PubMed ID: 24950685
5996  Rosario R, Araki H, Print CG, Shelling AN.  The transcriptional targets of mutant FOXL2 in granulosa cell tumours.  PLoS One  2012  7(9):e46270  PubMed ID: 23029457
7703  Kuo FT, Bentsi-Barnes IK, Barlow GM, Pisarska MD.  Mutant Forkhead L2 (FOXL2) proteins associated with premature ovarian failure (POF) dimerize with wild-type FOXL2, leading to altered regulation of genes associated with granulosa cell differentiation.  Endocrinology  2011  152:3917-29  PubMed ID: 21862621
8127  Horling K, Santos AN, Fischer B.  The AhR is constitutively activated and affects granulosa cell features in the human cell line KGN.  Mol. Hum. Reprod.  2011  17:104-14  PubMed ID: 20823264
8239  Zhang Y, Huang Q, Cheng JC, Nishi Y, Yanase T, Huang HF, Leung PC.  Homeobox A7 increases cell proliferation by up-regulation of epidermal growth factor receptor expression in human granulosa cells.  Reprod. Biol. Endocrinol.  2010  8:61  PubMed ID: 20540809
8474  Ohno S, Yukinawa F, Noda M, Nakajin S.  Mono-(2-ethylhexyl) phthalate induces NR4A subfamily and GIOT-1 gene expression, and suppresses CYP19 expression in human granulosa-like tumor cell line KGN.  Toxicol. Lett.  2009  191:353-9  PubMed ID: 19822197
8546  Komagata S, Nakajima M, Takagi S, Mohri T, Taniya T, Yokoi T.  Human CYP24 catalyzing the inactivation of calcitriol is post-transcriptionally regulated by miR-125b.  Mol. Pharmacol.  2009  76:702-9  PubMed ID: 19570947
6170  Mohri T, Nakajima M, Takagi S, Komagata S, Yokoi T.  MicroRNA regulates human vitamin D receptor.  Int J Cancer  2009  125(6):1328-33  PubMed ID: 19437538
3293  Tsuchiya Y, Nakajima M, Takagi S, Katoh M, Zheng W, Jefcoate CR, Yokoi T.  Binding of steroidogenic factor-1 to the regulatory region might not be critical for transcriptional regulation of the human CYP1B1 gene.  J. Biochem.  2006  139:527-34  PubMed ID: 16567417

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