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Cell No. : Cell Name
RCB1153 : S2 (Drosophila)  update : 2023/12/12
CommentFruit fly Oregon-R derived cell line. Highly sensitive in RNA interference compared with CHO-K1. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ueda, Ryu (Miyake, Tadashi)
Originator Schneider, I.
Year of deposit 1995
Another name Schneider line 2
Animal _drosophila < Insect
Genus Drosophila
Species melanogaster
Gender Unknown
Age at sampling embryo
Tissue embryo
Classification other
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_Z232
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium Schneider's + 10% FBS (heat inactivated) + 10μg/ml Insulin
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
deposit info
lot info
Reference information Reference 1
User's Publication 10

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4927  Schneider I.  Cell lines derived from late embryonic stages of Drosophila melanogaster.  J Embryol Exp Morphol  1972  27(2):353-65.  PubMed ID: 4625067  

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User's Publication
19872  Mori K, Nakagawa Y, Watanabe B, Miyata H, Morita T, Hayashi K.  Novel ability of diflubenzuron as an inhibitor of mitochondrial function  Insect Biochem Mol Biol  2024  167:104088  PubMed ID: 38342197   DOI: 10.1016/j.ibmb.2024.104088
13682  Chu WC, Hayashi S.  Mechano-chemical enforcement of tendon apical ECM into nano-filaments during Drosophila flight muscle development  Curr Biol  2021  S0960-9822(21)00043-9  PubMed ID: 33545042   DOI: 10.1016/j.cub.2021.01.010
20374  Kato S, Arasaki K, Tokutomi N, Imai Y, Inoshita T, Hattori N, Sasaki T, Sato M, Wakana Y, Inoue H, Tagaya M.  Syntaxin 17, an ancient SNARE paralog, plays different and conserved roles in different organisms.  J Cell Sci  2021    PubMed ID: 34704591   DOI: 10.1242/jcs.258699
11722  Uechi H, Kuranaga E.  The Tricellular Junction Protein Sidekick Regulates Vertex Dynamics to Promote Bicellular Junction Extension.  Dev. Cell  2019    PubMed ID: 31353316   DOI: 10.1016/j.devcel.2019.06.017
14055  Morishita S, Suzuki T, Niwa Y, Dohmae N, Simizu S.  Dpy-19 like 3-mediated C-mannosylation and expression levels of RPE-spondin in human tumor cell lines  Oncol Lett  2017  14(2):2537-2544  PubMed ID: 28781692   DOI: 10.3892/ol.2017.6465
14606  Nagahashi K, Umemura K, Kanayama N, Iwaki T.  Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells  Cytotechnology  2017  69(2):317-327  PubMed ID: 28070807   DOI: 10.1007/s10616-016-0059-y
7244  Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata T.  Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS.  Nat Commun  2015  6:8021  PubMed ID: 26282514   DOI: 10.1038/ncomms9021
18866  Koreishi M, Gniadek TJ, Yu S, Masuda J, Honjo Y, Satoh A.  The golgin tether giantin regulates the secretory pathway by controlling stack organization within Golgi apparatus  PLoS One  2013  8(3):e59821  PubMed ID: 23555793   DOI: 10.1371/journal.pone.0059821
8744  Kato A, Endo T, Abiko S, Ariga H, Matsumoto K.  Induction of truncated form of tenascin-X (XB-S) through dissociation of HDAC1 from SP-1/HDAC1 complex in response to hypoxic conditions.  Exp. Cell Res.  2008  314:2661-73  PubMed ID: 18588874   DOI: 10.1016/j.yexcr.2008.05.019
2490  Ui-Tei K, Zenno S, Miyata Y, Saigo K  Sensitive assay of RNA interference in Drosophila and Chinese hamster cultured cells using firefly luciferase gene as target.  FEBS Lett  2000  479:79-82  PubMed ID: 10981711   DOI: 10.1016/s0014-5793(00)01883-4

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