細胞番号 : 細胞名 RCB1152 : Sawano  update : 2023/07/25
細胞特性(Comment:英)			Human cell line derived from endometrial adenocarcinoma of uterus. Resistant to cisplatin.
細胞特性(日)			ヒト子宮内膜腺癌由来細胞株。シスプラチン耐性。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)			A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
使用条件(日)			営利目的か非営利目的かを問わず、いかなる使用につきましても、承諾者(寄託者)から承諾を得ることが必要です。
備考(英)
備考(日)
承諾者住所			× Japanese 住所 305-8577 茨城県つくば市天王台1-1-1 筑波大学   人間総合科学研究科（臨床医学系）疾患制御医学専攻 婦人周産期医学分野　産婦人科 佐藤 豊実 先生 Fax. 029-853-3072 English Address University of Tsukuba 1-1-1, Tenno-dai, Tsukuba, Ibaraki, 305-8577, Japan Dr.SATOH Toyomi Fax. +81-29-853-3072
提供申込書類(英)			Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日)			依頼書C-0001.pdf   承諾書C-0002.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf
			提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料			手数料とお支払いについてはこちらをご覧ください。
細胞基本情報		寄託者	Satoh, Toyomi
		樹立者	Satoh, Toyomi
		寄託日	1995
		動物種	_human < Mammals
		属名	Homo
		種名	sapiens
		人種	Japanese
		性別	Female
		齢	61 years
		採取組織	uterus
		病名	endometrial adenocarcinoma
		転移	Yes
		細胞分類	cancer
		初代培養日	1992
		細胞寿命	infinite
		細胞形態	epithelial-like
		Cellosaurus(Expasy)	CVCL_4898
細胞培養・検査情報			寄託時情報	ロット情報
	培地・試薬情報		培地・試薬一覧はこちらをご覧ください。
		培養形態		Adherent cells
		培地		MEM + 15% FBS
		抗生物質		Free
		継代方法		0.25% Trypsin
		継代密度		1 : 8 split
		継代・培地交換頻度		Subculture : 1-2 times/week, Medium Renewal : 2 times/week
		培養最適温度		37 ℃
		二酸化炭素濃度		5 %
		凍結培地		Medium + 10% DMSO
		凍結方法		Slow freezing
		マイコプラズマ		(-)
		ウィルス（HIV)		(-)
		アイソザイム検査		LD, NP
		染色体検査		41-92(50) : /44(3),45(9),46(7),47(10),48(11)/
		個体識別検査		OK
文献情報		Reference(英)	1件
		Reference(日)	0件
		利用者成果(英)	23件
		利用者成果(日)	0件

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Reference(英)

1977  Satoh T, Nishida M, Miyazaki Y, Sugita M, Arai Y, Oki A, Kono K, Tsunoda H, Kasahara K, Kubo T  [Establishment of a cisplatin-resistant new human endometrial adenocarcinoma cell line, Sawano cells]  Hum Cell  1995  8:67-72  PubMed ID: 8555123

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Reference(日)

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利用者成果(英)

21563  Konno T, Kohno T, Kikuchi S, Kura A, Saito K, Okada T, Shimada H, Yamazaki Y, Sugiyama T, Matsuura M, Ohsaki Y, Saito T, Kojima T.  The interplay between the epithelial permeability barrier, cell migration and mitochondrial metabolism of growth factors and their inhibitors in a human endometrial carcinoma cell line.  Tissue Barriers  2024  2304443  PubMed ID: 38225862   DOI: 10.1080/21688370.2024.2304443

20623  Shigeta S, Watanabe Y, Suzuki F, Nagase S, Shibuya Y, Ishibashi M, Nagai T, Shiga N, Toyoshima M, Tokunaga H, Shimada M, Yaegashi N.  MicroRNA-152 Regulates Endometrial Serous Carcinoma Cell Motility by Suppressing Matrix Metalloproteinase 10 Expression.  Tohoku J Exp Med  2022  256(3):249-258  PubMed ID: 35314531   DOI: 10.1620/tjem.256.249

20790  Saito K, Konno T, Kohno T, Shimada H, Matsuura M, Okada T, Kura A, Ishii D, Kondoh M, Saito T, Kojima T.  LSR antibody promotes apoptosis and disrupts epithelial barriers via signal pathways in endometrial cancer.  Tissue Barriers  2022  2106113  PubMed ID: 35883247   DOI: 10.1080/21688370.2022.2106113

16529  Kohno T, Konno T, Kikuchi S, Kondoh M, Kojima T.  Translocation of LSR from tricellular corners causes macropinocytosis at cell-cell interface as a trigger for breaking out of contact inhibition  FASEB J  2021  35(9):e21742  PubMed ID: 34403506   DOI: 10.1096/fj.202100299R

11947  Konno T, Kohno T, Kikuchi S, Shimada H, Satohisa S, Saito T, Kondoh M, Kojima T.  Epithelial barrier dysfunction and cell migration induction via JNK/cofilin/actin by angubindin-1.  Tissue Barriers  2020  8(1):1695475  PubMed ID: 31782346   DOI: 10.1080/21688370.2019.1695475

13069  Okada T, Konno T, Kohno T, Shimada H, Saito K, Satohisa S, Saito T, Kojima T.  Possibility of Targeting Claudin-2 in Therapy for Human Endometrioid Endometrial Carcinoma.  Reprod Sci  2020    PubMed ID: 32548807   DOI: 10.1007/s43032-020-00230-6

13627  Konno T, Kohno T, Okada T, Shimada H, Satohisa S, Kikuchi S, Saito T, Kojima T.  ASPP2 suppression promotes malignancy via LSR and YAP in human endometrial cancer  Histochem Cell Biol  2020  154(2):197-213.  PubMed ID: 32266459   DOI: 10.1007/s00418-020-01876-8

4346  Hashimoto C, Miki Y, Tanaka S, Takagi K, Fue M, Doe Z, Li B, Yaegashi N, Suzuki T, Ito K.  17β-Hydroxysteroid Dehydrogenase Type 2 Expression Is Induced by Androgen Signaling in Endometrial Cancer.  Int J Mol Sci  2018    PubMed ID: 29642629   DOI: 10.3390/ijms19041139

10060  Shimada H, Abe S, Kohno T, Satohisa S, Konno T, Takahashi S, Hatakeyama T, Arimoto C, Kakuki T, Kaneko Y, Takano KI, Saito T, Kojima T.  Loss of tricellular tight junction protein LSR promotes cell invasion and migration via upregulation of TEAD1/AREG in human endometrial cancer.  Sci Rep  2017  7:37049  PubMed ID: 28071680   DOI: 10.1038/srep37049

10303  Kohno T, Kikuchi S, Ninomiya T, Kojima T.  The bicellular tensile force sorts the localization of LSRs in bicellular and tricellular junctions.  Ann. N. Y. Acad. Sci.  2017  1397:185-194  PubMed ID: 28493278   DOI: 10.1111/nyas.13362

10532  Shimada H, Satohisa S, Kohno T, Konno T, Takano KI, Takahashi S, Hatakeyama T, Arimoto C, Saito T, Kojima T.  Downregulation of lipolysis-stimulated lipoprotein receptor promotes cell invasion via claudin-1-mediated matrix metalloproteinases in human endometrial cancer.  Oncol Lett  2017  14:6776-6782  PubMed ID: 29151917   DOI: 10.3892/ol.2017.7038

14530  Tsuji K, Utsunomiya H, Miki Y, Hanihara M, Fue M, Takagi K, Nishimoto M, Suzuki F, Yaegashi N, Suzuki T, Ito K.  Retinoic Acid Receptor β: A Potential Therapeutic Target in Retinoic Acid Treatment of Endometrial Cancer  Int J Gynecol Cancer  2017  27(4):643-650  PubMed ID: 28375930   DOI: 10.1097/IGC.0000000000000995

12976  Shimada H, Satohisa S, Kohno T, Takahashi S, Hatakeyama T, Konno T, Tsujiwaki M, Saito T, Kojima T.  The roles of tricellular tight junction protein lipolysis-stimulated lipoprotein receptor in malignancy of human endometrial cancer cells  Oncotarget  2016  7(19):27735-52  PubMed ID: 27036040   DOI: 10.18632/oncotarget.8408

8169  Egawa-Takata T, Endo H, Fujita M, Ueda Y, Miyatake T, Okuyama H, Yoshino K, Kamiura S, Enomoto T, Kimura T, Inoue M.  Early reduction of glucose uptake after cisplatin treatment is a marker of cisplatin sensitivity in ovarian cancer.  Cancer Sci.  2010  101:2171-8  PubMed ID: 20678156   DOI: 10.1111/j.1349-7006.2010.01670.x

8749  Matsumoto M, Yamaguchi Y, Seino Y, Hatakeyama A, Takei H, Niikura H, Ito K, Suzuki T, Sasano H, Yaegashi N, Hayashi S.  Estrogen signaling ability in human endometrial cancer through the cancer-stromal interaction.  Endocr. Relat. Cancer  2008  15:451-63  PubMed ID: 18508998   DOI: 10.1677/ERC-07-0227

9059  Y-C Jung, E Mizuki, T Akao, J-C Côté  Isolation and Characterization of a Novel Bacillus Thuringiensis Strain Expressing a Novel Crystal Protein With Cytocidal Activity Against Human Cancer Cells  J Appl Microbiol  2007  103 (1), 65-79  PubMed ID: 17584453   DOI: 10.1111/j.1365-2672.2006.03260.x

3413  Yamashita, Satoko, Katayama, Hideki, Saitoh, Hiroyuki, Akao, Tetsuyuki, Park, Yu Shin, Mizuki, Eiichi, Ohba, Michio, Ito, Akio  Typical Three-Domain Cry Proteins of Bacillus thuringiensis Strain A1462 Exhibit Cytocidal Activity on Limited Human Cancer Cells.  J Biochem  2005  138:663-72  PubMed ID: 16428294   DOI: 10.1093/jb/mvi177

2892  Katayama H, Yokota H, Akao T, Nakamura O, Ohba M, Mekada E, Mizuki E  Parasporin-1, a novel cytotoxic protein to human cells from non-insecticidal parasporal inclusions of Bacillus thuringiensis  J Biochem (Tokyo)  2005  137:17-25  PubMed ID: 15713879   DOI: 10.1093/jb/mvi003

9048  Shiro Okumura, Hiroyuki Saitoh, Tomoyuki Ishikawa, Naoya Wasano, Satoko Yamashita, Ken-Ichi Kusumoto, Tetsuyuki Akao, Eiichi Mizuki, Michio Ohba, Kuniyo Inouye  Identification of a Novel Cytotoxic Protein, Cry45Aa, From Bacillus Thuringiensis A1470 and Its Selective Cytotoxic Activity Against Various Mammalian Cell Lines  J Agric Food Chem  2005  53 (16), 6313-8  PubMed ID: 16076112   DOI: 10.1021/jf0506129

2894  Ito, Akio, Sasaguri, Yasuyuki, Kitada, Sakae, Kusaka, Yoshitomo, Kuwano, Kyoko, Masutomi, Kenjiro, Mizuki, Eiichi, Akao, Tetsuyuki, Ohba, Michio  A Bacillus thuringiensis crystal protein with selective cytocidal action to human cells.  J Biol Chem  2004  279:21282-6  PubMed ID: 15026424   DOI: 10.1074/jbc.M401881200

3101  Matsushima-Nishiu M, Unoki M, Ono K, Tsunoda T, Minaguchi T, Kuramoto H, Nishida M, Satoh T, Tanaka T, Nakamura Y  Growth and gene expression profile analyses of endometrial cancer cells expressing exogenous PTEN.  Cancer Res  2001  61:3741-9  PubMed ID: 11325847

6166  Unoki M, Nakamura Y.  Growth-suppressive effects of BPOZ and EGR2, two genes involved in the PTEN signaling pathway.  Oncogene  2001  20(33):4457-65  PubMed ID: 11494141   DOI: 10.1038/sj.onc.1204608

17275  Fan H, Morioka T, Ito E.  Induction of apoptosis and growth inhibition of cultured human endometrial adenocarcinoma cells (Sawano) by an antitumor lipoprotein fraction of rice bran  Gynecol Oncol  2000  76(2):170-5  PubMed ID: 10637066   DOI: 10.1006/gyno.1999.5669

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