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Cell No. : Cell Name
RCB1145 : 32D  update : 2018/07/06
CommentIL-3 dependent cells from Friend leukemia virus-infected mouse bone marrow.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Todokoro, Kazuo
Year of deposit 1995
Animal mouse < Mammals
Strain name C3H/HeJ
Tissue bone marrow
Case history Friend leukemia
Classification transformed
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 10U/ml rmouse IL-3
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10-20 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 6
User's Publication 1

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1980  Reisbach G, Ellwart J, Dormer P  Lactate production and amino acid incorporation in interleukin 3-dependent, factor-deprived hemopoietic murine cell lines.  Exp Cell Res  1990  190:175-8  PubMed ID: 2209720
5466  Migliaccio G, Migliaccio AR, Kreider BL, Rovera G, Adamson JW.  Selection of lineage-restricted cell lines immortalized at different stages of hematopoietic differentiation from the murine cell line 32D.  J Cell Biol  1989  109(2):833-41  PubMed ID: 2668305
5467  Pierce JH, Ruggiero M, Fleming TP, Di Fiore PP, Greenberger JS, Varticovski L, Schlessinger J, Rovera G, Aaronson SA.  Signal transduction through the EGF receptor transfected in IL-3-dependent hematopoietic cells.  Science  1988  239(4840):628-31  PubMed ID: 3257584
1983  Le Gros, G S, Shackell, P S, Le Gros, J E, Watson, J D  Interleukin 2 regulates the expression of IL 2 receptors on interleukin 3-dependent bone marrow-derived cell lines.  J Immunol  1987  138:478-83  PubMed ID: 3098845
4924  Tweardy DJ1, Caracciolo D, Valtieri M, Rovera G.  Tumor-derived growth factors that support proliferation and differentiation of normal and leukemic hemopoietic cells.  Ann N Y Acad Sci  1987  511:30-8  PubMed ID: 2830826
1979  Greenberger, J S, Sakakeeny, M A, Humphries, R K, Eaves, C J, Eckner, R J  Demonstration of permanent factor-dependent multipotential (erythroid/neutrophil/basophil) hematopoietic progenitor cell lines.  Proc Natl Acad Sci U S A  1983  80:2931-5  PubMed ID: 6574462

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User's Publication
7107  Yamazoe H, Ichikawa T, Hagihara Y, Iwasaki Y.  Generation of a patterned co-culture system composed of adherent cells and immobilized nonadherent cells.  Acta Biomater  2015  31:231-240  PubMed ID: 26685756

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