Cell No. : Cell Name
RCB1144 : DA-3
update : 2024/01/17
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| Comment | IL-3 dependent mouse myeloid cells. MoLV induced. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Todokoro, Kazuo
|
| Year of deposit |
1995
|
| Animal |
_mouse
< Mammals
|
| Tissue |
myeloid
|
| Disease name |
Moloney murine leukemia
|
| Classification |
transformed
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_5419
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS + 10U/ml rmouse IL-3
|
| Antibiotics |
|
Free
|
|
Passage method |
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dilution
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Culture information
|
Passage ratio |
|
1 : 10-20 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
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| Isozyme |
|
LD, NP
|
| Reference information |
Reference |
3
|
| User's Publication |
4
|
| Reference |
|
1980
Reisbach G, Ellwart J, Dormer P
Lactate production and amino acid incorporation in interleukin 3-dependent, factor-deprived hemopoietic murine cell lines.
Exp Cell Res
1990
190:175-8
PubMed ID: 2209720
DOI: 10.1016/0014-4827(90)90182-a
|
|
1982
Hultner L, Szots H, Van Snick J, Moeller J, Welle M, Dormer P
Interleukin 3 mediates interleukin 6 production in murine interleukin 3-dependent hemopoietic cells.
Growth Factors
1989
2:43-51
PubMed ID: 2635055
DOI: 10.3109/08977198909069080
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|
1981
Pope, B L, Djeu, J Y, Almawi, W Y
Secretion of a suppressor cell inducing factor by an interleukin 3-dependent cell line with natural cytotoxic activity. III. Comparison with other interleukin 3-dependent cell lines.
Cell Immunol
1988
116:1-11
PubMed ID: 2971453
DOI: 10.1016/0008-8749(88)90204-3
|
| User's Publication |
|
8207
Kaori Yoshino, Kaoru Saijo, Chikako Noro and Yukio Nakamura
Development of a simple method to determine the mouse strain from which cultured cell lines originated
Interdisciplinary Bio Central
2010
2:0014
DOI: 10.4051/ibc.2010.2.4.0014
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18143
Nogami W, Yoshida H, Koizumi K, Yamada H, Abe K, Arimura A, Yamane N, Takahashi K, Yamane A, Oda A, Tanaka Y, Takemoto H, Ohnishi Y, Ikeda Y, Miyakawa Y.
The effect of a novel, small non-peptidyl molecule butyzamide on human thrombopoietin receptor and megakaryopoiesis
Haematologica
2008
93(10):1495-504
PubMed ID: 18728031
DOI: 10.3324/haematol.12752
|
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2951
Yokota S, Kayano T, Ohta T, Kurimoto M, Yanagi H, Yura T, Kubota H
Proteasome-dependent degradation of cytosolic chaperonin CCT
Biochemical and biophysical research communications
2000
279:712-717
PubMed ID: 11118350
DOI: 10.1006/bbrc.2000.4011
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2380
Yokota S, Yanagi H, Yura T, Kubota H
Cytosolic chaperonin is up-regulated during cell growth. Preferential expression and binding to tubulin at G(1)/S transition through early S phase.
J Biol Chem
1999
274:37070-8
PubMed ID: 10601265
DOI: 10.1074/jbc.274.52.37070
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