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Cell No. : Cell Name
RCB1144 : DA-3  update : 2022/03/29
CommentIL-3 dependent mouse myeloid cells. MoLV induced.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Todokoro, Kazuo
Year of deposit 1995
Animal mouse < Mammals
Tissue myeloid
Disease name Moloney murine leukemia
Classification transformed
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_5419
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 10U/ml rmouse IL-3
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10-20 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 3
User's Publication 3

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1980  Reisbach G, Ellwart J, Dormer P  Lactate production and amino acid incorporation in interleukin 3-dependent, factor-deprived hemopoietic murine cell lines.  Exp Cell Res  1990  190:175-8  PubMed ID: 2209720  
1982  Hultner L, Szots H, Van Snick J, Moeller J, Welle M, Dormer P  Interleukin 3 mediates interleukin 6 production in murine interleukin 3-dependent hemopoietic cells.  Growth Factors  1989  2:43-51  PubMed ID: 2635055  
1981  Pope, B L, Djeu, J Y, Almawi, W Y  Secretion of a suppressor cell inducing factor by an interleukin 3-dependent cell line with natural cytotoxic activity. III. Comparison with other interleukin 3-dependent cell lines.  Cell Immunol  1988  116:1-11  PubMed ID: 2971453  

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User's Publication
8207  Kaori Yoshino, Kaoru Saijo, Chikako Noro and Yukio Nakamura  Development of a simple method to determine the mouse strain from which cultured cell lines originated  Interdisciplinary Bio Central   2010  2:0014    DOI: 10.4051/ibc.2010.2.4.0014
2951  Yokota S, Kayano T, Ohta T, Kurimoto M, Yanagi H, Yura T, Kubota H  Proteasome-dependent degradation of cytosolic chaperonin CCT  Biochemical and biophysical research communications  2000  279:712-717  PubMed ID: 11118350   DOI: 10.1006/bbrc.2000.4011
2380  Yokota S, Yanagi H, Yura T, Kubota H  Cytosolic chaperonin is up-regulated during cell growth. Preferential expression and binding to tubulin at G(1)/S transition through early S phase.  J Biol Chem  1999  274:37070-8  PubMed ID: 10601265   DOI: 10.1074/jbc.274.52.37070

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