Cell No. : Cell Name
RCB1143 : DA-1
update : 2024/09/17
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| Comment | IL-3 dependent mouse myeloid cells. MoLV induced. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Todokoro, Kazuo
|
| Year of deposit |
1995
|
| Animal |
_mouse
< Mammals
|
| Strain name |
BALB/c
|
| Tissue |
myeloid
|
| Disease name |
Moloney murine leukemia
|
| Classification |
transformed
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
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| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_2015
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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RPMI1640 + 10% FBS + 10U/ml rmouse IL-3
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10-20 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| SSLP(mouse) |
|
OK
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| Isozyme |
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LD, NP, G6PD
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
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| User's Publication |
1
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| Reference |
|
1980
Reisbach G, Ellwart J, Dormer P
Lactate production and amino acid incorporation in interleukin 3-dependent, factor-deprived hemopoietic murine cell lines.
Exp Cell Res
1990
190:175-8
PubMed ID: 2209720
DOI: 10.1016/0014-4827(90)90182-a
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1982
Hultner L, Szots H, Van Snick J, Moeller J, Welle M, Dormer P
Interleukin 3 mediates interleukin 6 production in murine interleukin 3-dependent hemopoietic cells.
Growth Factors
1989
2:43-51
PubMed ID: 2635055
DOI: 10.3109/08977198909069080
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1981
Pope, B L, Djeu, J Y, Almawi, W Y
Secretion of a suppressor cell inducing factor by an interleukin 3-dependent cell line with natural cytotoxic activity. III. Comparison with other interleukin 3-dependent cell lines.
Cell Immunol
1988
116:1-11
PubMed ID: 2971453
DOI: 10.1016/0008-8749(88)90204-3
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1978
Pierce, J H, Di Fiore, P P, Aaronson, S A, Potter M, Pumphrey J, Scott A, Ihle, J N
Neoplastic transformation of mast cells by Abelson-MuLV: abrogation of IL-3 dependence by a nonautocrine mechanism.
Cell
1985
41:685-93
PubMed ID: 2988783
DOI: 10.1016/s0092-8674(85)80049-0
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