Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB1142 : 10H-2  update : 2022/03/29
CommentAnti poly(ADP-ribose) IgG3 kappa producing.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochemistry 1984;23:3771-3777) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miwa, Masanao
Originator Miwa, Masanao
Year of deposit 1994
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_N539
hybridoma Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_1 gender Female
Fusion_cell_2 name NS-1
Fusion_cell_2 strain mouse
Fusion method Kohler & Milstein method
Antibody name anti poly ADP ribose
Subclass IgG3
Immunogen poly(ADP-rib)
Antigen_name poly ADP ribose
Antigen_m.w. 10000〜200000
Antigen_epitope poly ADP ribose
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM (low glucose) + 10mM HEPES + 10% FBS + 2% NCTC135 + 1mM Sodium Pyruvate + 0.1mM NEAA + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG3
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


To topTop
Reference
2839  Masutani, Mitsuko, Miwa, Masanao  Poly(ADP-ribose) polymerase and cancer: in relation to the lectures presented by Dr Gilbert de Murcia.  Jpn J Clin Oncol  2002  32:483-7  PubMed ID: 12499423
2840  Soldani C, Lazze, M C, Bottone, M G, Tognon G, Biggiogera M, Pellicciari, C E, Scovassi, A I  Poly(ADP-ribose) polymerase cleavage during apoptosis: when and where?  Exp Cell Res  2001  269:193-201  PubMed ID: 11570811
1788  Kawamitsu H, Hoshino H, Okada H, Miwa M, Momoi H, Sugimura T  Monoclonal antibodies to poly(adenosine diphosphate ribose) recognize different structures.  Biochemistry  1984  23:3771-7  PubMed ID: 6206890

To topTop
User's Publication



Back Back Return Top Page