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Cell No. : Cell Name
RCB1142 : 10H-2  update : 2024/01/17
CommentAnti poly(ADP-ribose) IgG3 kappa producing.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochemistry 1984;23:3771-3777) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miwa, Masanao
Originator Miwa, Masanao
Year of deposit 1994
Animal _mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_N539
hybridoma Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_1 gender Female
Fusion_cell_2 name NS-1
Fusion_cell_2 strain mouse
Fusion method Kohler & Milstein method
Antibody name anti poly ADP ribose
Subclass IgG3
Immunogen poly(ADP-rib)
Antigen_name poly ADP ribose
Antigen_m.w. 10000〜200000
Antigen_epitope poly ADP ribose
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method General Guidelines for Culturing Suspension Cells
浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium DMEM (low glucose) + 10mM HEPES + 10% FBS + 2% NCTC135 + 1mM Sodium Pyruvate + 0.1mM NEAA + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Antibody subclass IgG3
Images
deposit info
lot info
Reference information Reference 8
User's Publication 1


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Reference
20973  Ida C, Yamashita S, Eguchi T, Kuroda Y, Nakae S, Nishi Y, Kamemura K, Shirai T, Mizukami T, Tanaka M, Moss J, Miwa M.  An Enzyme-Linked Immunosorbent Assay to Quantify Poly (ADP-Ribose) Level In Vivo.  Methods Mol Biol  2022  2609:91-100  PubMed ID: 36515831   DOI: 10.1007/978-1-0716-2891-1_6
857  Ida C, Yamashita S, Tsukada M, Sato T, Eguchi T, Tanaka M, Ogata S, Fujii T, Nishi Y, Ikegami S, Moss J, Miwa M.   An enzyme-linked immunosorbent assay-based system for determining the physiological level of poly(ADP-ribose) in cultured cells  Anal Biochem  2016  494:76-81  PubMed ID: 26548958   DOI: 10.1016/j.ab.2015.10.014
855  Shitara Y, Tonohora Y, Goto T, Yamada Y, Miki T, Makino H, Miwa M, Komiya T.  Mitochondrial P5, a member of protein disulphide isomerase family, suppresses oxidative stress-induced cell death  J Biochem   2012  152(1):73-85  PubMed ID: 22492663   DOI: 10.1093/jb/mvs034
938  Kanai M, Tong WM, Wang ZQ, Miwa M.  Haploinsufficiency of poly(ADP-ribose) polymerase-1-mediated poly(ADP-ribosyl)ation for centrosome duplication  Biochem Biophys Res Commun  2007  359(3):426-30  PubMed ID: 17553458   DOI: 10.1016/j.bbrc.2007.05.108
856  Kanai M, Tong WM, Sugihara E, Wang ZQ, Fukasawa K, Miwa M.   Involvement of poly(ADP-Ribose) polymerase 1 and poly(ADP-Ribosyl)ation in regulation of centrosome function  Mol Cell Biol  2003  23(7):2451-62  PubMed ID: 12640128   DOI: 10.1128/MCB.23.7.2451-2462.2003
2839  Masutani, Mitsuko, Miwa, Masanao  Poly(ADP-ribose) polymerase and cancer: in relation to the lectures presented by Dr Gilbert de Murcia.  Jpn J Clin Oncol  2002  32:483-7  PubMed ID: 12499423   DOI: 10.1093/jjco/hyf101
2840  Soldani C, Lazze, M C, Bottone, M G, Tognon G, Biggiogera M, Pellicciari, C E, Scovassi, A I  Poly(ADP-ribose) polymerase cleavage during apoptosis: when and where?  Exp Cell Res  2001  269:193-201  PubMed ID: 11570811   DOI: 10.1006/excr.2001.5293
1788  Kawamitsu H, Hoshino H, Okada H, Miwa M, Momoi H, Sugimura T  Monoclonal antibodies to poly(adenosine diphosphate ribose) recognize different structures.  Biochemistry  1984  23:3771-7  PubMed ID: 6206890   DOI: 10.1021/bi00311a032

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User's Publication
19331  Huang JY, Chen WH, Chang YL, Wang HT, Chuang WT, Lee SC.  Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation  Nucleic Acids Res  2006  34(8):2398-407  PubMed ID: 16682447   DOI: 10.1093/nar/gkl241


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