Cell No. : Cell Name
RCB1142 : 10H-2
update : 2024/01/17
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| Comment | Anti poly(ADP-ribose) IgG3 kappa producing. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochemistry 1984;23:3771-3777) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Miwa, Masanao
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| Originator |
Miwa, Masanao
|
| Year of deposit |
1994
|
| Animal |
_mouse x mouse
< Hybrid
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| Tissue |
hybridoma
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| Classification |
hybrid cells
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|
Lifespan |
infinite
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| Morphology |
lymphocyte-like
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| Cellosaurus(Expasy) |
CVCL_N539
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hybridoma
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Fusion_cell_1 strain |
mouse, BALB/c
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| Fusion_cell_1 gender |
Female
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| Fusion_cell_2 name |
NS-1
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| Fusion_cell_2 strain |
mouse
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|
Fusion method |
Kohler & Milstein method
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Antibody name |
anti poly ADP ribose
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| Subclass |
IgG3
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| Immunogen |
poly(ADP-rib)
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|
Antigen_name |
poly ADP ribose
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| Antigen_m.w. |
10000〜200000
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| Antigen_epitope |
poly ADP ribose
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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DMEM (low glucose) + 10mM HEPES + 10% FBS + 2% NCTC135 + 1mM Sodium Pyruvate + 0.1mM NEAA + 50μM 2-Mercaptoethanol
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Antibody subclass |
|
IgG3
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
8
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| User's Publication |
1
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| Reference |
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20973
Ida C, Yamashita S, Eguchi T, Kuroda Y, Nakae S, Nishi Y, Kamemura K, Shirai T, Mizukami T, Tanaka M, Moss J, Miwa M.
An Enzyme-Linked Immunosorbent Assay to Quantify Poly (ADP-Ribose) Level In Vivo.
Methods Mol Biol
2022
2609:91-100
PubMed ID: 36515831
DOI: 10.1007/978-1-0716-2891-1_6
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857
Ida C, Yamashita S, Tsukada M, Sato T, Eguchi T, Tanaka M, Ogata S, Fujii T, Nishi Y, Ikegami S, Moss J, Miwa M.
An enzyme-linked immunosorbent assay-based system for determining the physiological level of poly(ADP-ribose) in cultured cells
Anal Biochem
2016
494:76-81
PubMed ID: 26548958
DOI: 10.1016/j.ab.2015.10.014
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855
Shitara Y, Tonohora Y, Goto T, Yamada Y, Miki T, Makino H, Miwa M, Komiya T.
Mitochondrial P5, a member of protein disulphide isomerase family, suppresses oxidative stress-induced cell death
J Biochem
2012
152(1):73-85
PubMed ID: 22492663
DOI: 10.1093/jb/mvs034
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938
Kanai M, Tong WM, Wang ZQ, Miwa M.
Haploinsufficiency of poly(ADP-ribose) polymerase-1-mediated poly(ADP-ribosyl)ation for centrosome duplication
Biochem Biophys Res Commun
2007
359(3):426-30
PubMed ID: 17553458
DOI: 10.1016/j.bbrc.2007.05.108
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856
Kanai M, Tong WM, Sugihara E, Wang ZQ, Fukasawa K, Miwa M.
Involvement of poly(ADP-Ribose) polymerase 1 and poly(ADP-Ribosyl)ation in regulation of centrosome function
Mol Cell Biol
2003
23(7):2451-62
PubMed ID: 12640128
DOI: 10.1128/MCB.23.7.2451-2462.2003
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2839
Masutani, Mitsuko, Miwa, Masanao
Poly(ADP-ribose) polymerase and cancer: in relation to the lectures presented by Dr Gilbert de Murcia.
Jpn J Clin Oncol
2002
32:483-7
PubMed ID: 12499423
DOI: 10.1093/jjco/hyf101
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2840
Soldani C, Lazze, M C, Bottone, M G, Tognon G, Biggiogera M, Pellicciari, C E, Scovassi, A I
Poly(ADP-ribose) polymerase cleavage during apoptosis: when and where?
Exp Cell Res
2001
269:193-201
PubMed ID: 11570811
DOI: 10.1006/excr.2001.5293
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1788
Kawamitsu H, Hoshino H, Okada H, Miwa M, Momoi H, Sugimura T
Monoclonal antibodies to poly(adenosine diphosphate ribose) recognize different structures.
Biochemistry
1984
23:3771-7
PubMed ID: 6206890
DOI: 10.1021/bi00311a032
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