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Cell No. : Cell Name
RCB1139 : HFSKF-AE-V  update : 2022/05/12
CommentHuman fibroblasts derived from fetal skin (31 weekly age).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishiwata, Isamu
Originator Ishiwata, I. & Ishikawa, H.
Year of deposit 1994
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling embryo
Tissue embryo/fetus, skin
Classification normal
Year of origin 1994
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5513
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 46(50)
STR(human) OK
Reference information Reference 0
User's Publication 2


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Reference

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User's Publication
3579  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504
3085  Shimizu, Makiko, Matsuda, Akira, Yanagisawa, Ken, Hirota, Tomomitsu, Akahoshi, Mitsuteru, Inomata, Naoko, Ebe, Kouji, Tanaka, Keiko, Sugiura, Hisashi, Nakashima, Kazuko, Tamari, Mayumi, Takahashi, Naomi, Obara, Kazuhiko, Enomoto, Tadao, Okayama, Yoshimichi, Gao, Pei-Song, Huang, Shau-Ku, Tominaga, Shin-Ichi, Ikezawa, Zenro, Shirakawa, Taro  Functional SNPs in the distal promoter of the ST2 gene are associated with atopic dermatitis.  Hum Mol Genet  2005  14:2919-27  PubMed ID: 16118232   DOI: 10.1093/hmg/ddi323



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