Back Back
Cell No. : Cell Name
RCB1127 : MC3T3-G2/PA6  update : 2024/06/05
CommentSupport hematpoietic stem cell and osteoclast differentiation. Pre-adipocyte.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kodama, Hiroaki
Originator Kodama, Hiroaki
Year of deposit 1994
Another name PA6
Original cell MC3T3-G2
Animal _mouse < Mammals
Strain name C57BL/6
Gender Unknown
Age at sampling 0 day
Tissue newborn, calvaria
Classification other
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5441
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 5
User's Publication 117

To topTop
2436  Nishikawa S, Ogawa M, Nishikawa S, Kunisada T, Kodama H.  B lymphopoiesis on stromal cell clone: stromal cell clones acting on different stages of B cell differentiation  Eur J Immunol  1998  18(11):1767-71  PubMed ID: 3264531   DOI: 10.1002/eji.1830181117
2437  Hayashi S, Kunisada T, Ogawa M, Sudo T, Kodama H, Suda T, Nishikawa S, Nishikawa S.  Stepwise progression of B lineage differentiation supported by interleukin 7 and other stromal cell molecules  J Exp Med  1990  171(5):1683-95  PubMed ID: 2332734   DOI: 10.1084/jem.171.5.1683
2435  Udagawa N, Takahashi N, Akatsu T, Sasaki T, Yamaguchi A, Kodama H, Martin TJ, Suda T.  The bone marrow-derived stromal cell lines MC3T3-G2/PA6 and ST2 support osteoclast-like cell differentiation in cocultures with mouse spleen cells  Endocrinology  1989  125(4):1805-13  PubMed ID: 2676473   DOI: 10.1210/endo-125-4-1805
1846  Kodama, H A, Amagai Y, Koyama H, Kasai S  Hormonal responsiveness of a preadipose cell line derived from newborn mouse calvaria.  J Cell Physiol  1982  112:83-8  PubMed ID: 7107717   DOI: 10.1002/jcp.1041120113
1847  Kodama, H A, Amagai Y, Koyama H, Kasai S  A new preadipose cell line derived from newborn mouse calvaria can promote the proliferation of pluripotent hemopoietic stem cells in vitro.  J Cell Physiol  1982  112:89-95  PubMed ID: 7107718   DOI: 10.1002/jcp.1041120114

To topTop
User's Publication
21597  Kim JH, Yang J, Ki MG, Jeon DH, Kim JW, Jang M, Lee G.  Differentiation of Human induced Pluripotent Stem Cell-derived Dental Stem Cells through Epithelial-Mesenchymal Interaction.  Stem Cells Dev  2024    PubMed ID: 38366745   DOI: 10.1089/scd.2023.0220
19784  Wenker SD, Farias MI, Gradaschi V, Garcia C, Beauquis J, Leal MC, Ferrari C, Zeng X, Pitossi FJ.  Microglia-secreted TNF-α affects differentiation efficiency and viability of pluripotent stem cell-derived human dopaminergic precursors  PLoS One  2023  18(9):e0263021  PubMed ID: 37751438   DOI: 10.1371/journal.pone.0263021
20326  Gualandris A, Noghero A, Cora' D, Astanina E, Arese M, Bussolino F.  Role of TGFβ1 and WNT6 in FGF2 and BMP4-driven endothelial differentiation of murine embryonic stem cells.  Angiogenesis  2022    PubMed ID: 34478025   DOI: 10.1007/s10456-021-09815-4
20769  Yamazoe H, Kurinomaru T, Inagaki A.  Potential of the Coordinated Actions of Multiple Protein-Based Micromachines for Medical Applications.  ACS Appl Mater Interfaces  2022    PubMed ID: 35822220   DOI: 10.1021/acsami.2c08223
20054  Tomita H, Tanaka K, Hirata A, Okada H, Imai H, Shirakami Y, Ohnishi K, Sugie S, Aoki H, Hatano Y, Noguchi K, Kanayama T, Niwa A, Suzui N, Miyazaki T, Tanaka T, Akiyama H, Shimizu M, Yoshida K, Hara A.  Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas.  Cell Rep  2021  34(8):108772  PubMed ID: 33626352   DOI: 10.1016/j.celrep.2021.108772
14070  Kondo T.  Selective eradication of pluripotent stem cells by inhibiting DHODH activity  Stem Cells  2021  39(1):33-42  PubMed ID: 33038285   DOI: 10.1002/stem.3290
14511  Wei M, Li S, Yang Z, Cheng C, Li T, Le W.  Tetrahedral DNA nanostructures functionalized by multivalent microRNA132 antisense oligonucleotides promote the differentiation of mouse embryonic stem cells into dopaminergic neurons  Nanomedicine  2021  34:102375  PubMed ID: 33617970   DOI: 10.1016/j.nano.2021.102375
20508  Lee HS, Mok J, Joo CK.  Bone Morphogenetic Protein 4 (BMP4) Enhances the Differentiation of Human Induced Pluripotent Stem Cells into Limbal Progenitor Cells.  Curr Issues Mol Biol  2021  43(3):2124-2134  PubMed ID: 34940121   DOI: 10.3390/cimb43030147
16763  Kitamura N, Fujiwara N, Hayakawa K, Ohama T, Sato K.  Protein phosphatase 6 promotes neurite outgrowth by promoting mTORC2 activity in N2a cells  J Biochem  2021  170(1):131-138  PubMed ID: 34314486   DOI: 10.1093/jb/mvab028
12175  Hiroki Masuda, Yoshinori Arisaka, Ruriko Sekiya-Aoyama, Tetsuya Yoda, Nobuhiko Yui   Biological Effects of Polyrotaxane Surfaces on Cellular Responses of Fibroblast, Preosteoblast and Preadipocyte Cell Lines  Polymers  2020    PubMed ID: 32316349   DOI: 10.3390/polym12040924
11367  Ishijima Y, Ohmori S, Uneme A, Aoki Y, Kobori M, Ohida T, Arai M, Hosaka M, Ohneda K.  The Gata2 repression during 3T3-L1 preadipocyte differentiation is dependent on a rapid decrease in histone acetylation in response to glucocorticoid receptor activation.  Mol. Cell. Endocrinol.  2019    PubMed ID: 30615908   DOI: 10.1016/j.mce.2019.01.002
11968  Hayakawa K, Nishitani K, Tanaka S.  Kynurenine, 3-OH-kynurenine, and anthranilate are nutrient metabolites that alter H3K4 trimethylation and H2AS40 O-GlcNAcylation at hypothalamus-related loci.  Sci Rep  2019    PubMed ID: 31875008   DOI: 10.1038/s41598-019-56341-x
12480  Paerhati P, Ito A, Yoshioka K, Iwamoto K, Fujiwara S, Horie M, Kawabe Y, Kamihira M.  Neural differentiation of mouse induced pluripotent stem cells using cadherin gene-engineered PA6 feeder cells  J Biosci Bioeng  2019  127(5):633-640  PubMed ID: 30391238   DOI: 10.1016/j.jbiosc.2018.10.009
10471  Nugroho AE, Hashimoto A, Wong CP, Yokoe H, Tsubuki M, Kaneda T, Hadi AHA, Morita H.  Ceramicines M-P from Chisocheton ceramicus: isolation and structure-activity relationship study.  J Nat Med  2018  72:64-72  PubMed ID: 28822030   DOI: 10.1007/s11418-017-1109-2
4284  Carr-Wilkinson J, Prathalingam N, Pal D, Moad M, Lee N, Sundaresh A, Forgham H, James P, Herbert M, Lako M, Tweddle DA.  Differentiation of Human Embryonic Stem Cells to Sympathetic Neurons: A Potential Model for Understanding Neuroblastoma Pathogenesis.  Stem Cells Int  2018    PubMed ID: 30515222   DOI: 10.1155/2018/4391641
4285  Méndez-Maldonado K, Vega-López G, Caballero-Chacón S, Aybar MJ, Velasco I.  Activation of Hes1 and Msx1 in Transgenic Mouse Embryonic Stem Cells Increases Differentiation into Neural Crest Derivatives.  Int J Mol Sci  2018    PubMed ID: 30551562   DOI: 10.3390/ijms19124025
4492  Joshi R, Fuller B, Mosadegh B, Tavana H.  Stem cell colony interspacing effect on differentiation to neural cells.  J Tissue Eng Regen Med  2018  12(10):2041-2054  PubMed ID: 30058271   DOI: 10.1002/term.2739
10340  Ogawa Y, Kaizu K, Yanagi Y, Takada S, Sakuraba H, Oishi K.  Abnormal differentiation of Sandhoff disease model mouse-derived multipotent stem cells toward a neural lineage.  PLoS ONE  2017  12:e0178978  PubMed ID: 28575132   DOI: 10.1371/journal.pone.0178978
5771  Hayakawa K, Sakamoto Y, Kanie O, Ohtake A, Daikoku S, Ito Y, Shiota K.  Reactivation of hyperglycemia-induced hypocretin (HCRT) gene silencing by N-acetyl-d-mannosamine in the orexin neurons derived from human iPS cells.  Epigenetics  2017  12(9):764-778  PubMed ID: 28762874   DOI: 10.1080/15592294.2017.1346775
9457  Junho Kim, Chugmin Han, Wonju Jo, Sehong Kang, Siwoo Cho, Dayeong Jeong, Yong Song Gho, Jaesung Park  Cell-Engineered Nanovesicle as a Surrogate Inducer of Contact-Dependent Stimuli  Adv Healthc Mater  2017  6(17)  PubMed ID: 28643483   DOI: 10.1002/adhm.201700381
14048  Wei M, Li S, Yang Z, Zheng W, Le W.  Gold nanoparticles enhance the differentiation of embryonic stem cells into dopaminergic neurons via mTOR/p70S6K pathway  Nanomedicine (Lond)  2017  12(11):1305-1317  PubMed ID: 28520507   DOI: 10.2217/nnm-2017-0001
14569  Newman EA, Chukkapalli S, Bashllari D, Thomas TT, Van Noord RA, Lawlor ER, Hoenerhoff MJ, Opipari AW, Opipari VP.  Alternative NHEJ pathway proteins as components of MYCN oncogenic activity in human neural crest stem cell differentiation: implications for neuroblastoma initiation  Cell Death Dis  2017  8(12):3208  PubMed ID: 29238067   DOI: 10.1038/s41419-017-0004-9
14723  Nakajima K, So M, Takahashi K, Tagawa YI, Hirao M, Goto Y, Ogi H.  Optimized Ultrasonic Irradiation Finds Out Ultrastable Aβ 1-40 Oligomers  J Phys Chem B  2017  121(12):2603-2613  PubMed ID: 28260388   DOI: 10.1021/acs.jpcb.7b01409
14776  Joshi R, Buchanan JC, Tavana H.  Self-regulatory factors of embryonic stem cells in co-culture with stromal cells enhance neural differentiation  Integr Biol (Camb)  2017  9(5):418-426  PubMed ID: 28406502   DOI: 10.1039/c7ib00038c
10023  Gökcan MK, Mülazimoğlu S, Ocak E, Can P, Çalışkan M, Beşaltı Ö, Dizbay Sak S, Kaygusuz G.  Study of mouse induced pluripotent stem cell transplantation intoWistar albino rat cochleae after hair cell damage.  Turk J Med Sci  2016  46:1603-1610  PubMed ID: 27966334   DOI: 10.3906/sag-1510-136
6004  Hertsenberg AJ, Funderburgh JL.  Generation of Corneal Keratocytes from Human Embryonic Stem Cells.  Methods Mol Biol  2016  1341:285-94  PubMed ID: 26026882   DOI: 10.1007/7651_2015_231
9431  Anita Bonic, Mack Poon, Michael G Woods, Alan O Trounson  Expression of Neural Crest Markers by Human Embryonic Stem Cells: An Introductory Project  Aust Orthod J  2016  32(2):175-183.  PubMed ID: 29509342  
12908  Zhang JT, Weng ZH, Tsang KS, Tsang LL, Chan HC, Jiang XH.  MycN Is Critical for the Maintenance of Human Embryonic Stem Cell-Derived Neural Crest Stem Cells  PLoS One  2016  11(1):e0148062  PubMed ID: 26815535   DOI: 10.1371/journal.pone.0148062
13952  Hedlund E, Belnoue L, Theofilopoulos S, Salto C, Bye C, Parish C, Deng Q, Kadkhodaei B, Ericson J, Arenas E, Perlmann T, Simon A.  Dopamine Receptor Antagonists Enhance Proliferation and Neurogenesis of Midbrain Lmx1a-expressing Progenitors  Sci Rep  2016  6:26448  PubMed ID: 27246266   DOI: 10.1038/srep26448
14037  Joshi R, Buchanan JC, Paruchuri S, Morris N, Tavana H.  Molecular Analysis of Stromal Cells-Induced Neural Differentiation of Mouse Embryonic Stem Cells  PLoS One  2016  11(11):e0166316  PubMed ID: 27832161   DOI: 10.1371/journal.pone.0166316
14038  Joshi R, Buchanan J, Tavana H.  Colony size effect on neural differentiation of embryonic stem cells microprinted on stromal cells  Annu Int Conf IEEE Eng Med Biol Soc  2016  4173-4176  PubMed ID: 28269202   DOI: 10.1109/EMBC.2016.7591646
7223  Arai Y, Hayakawa K, Arai D, Ito R, Iwasaki Y, Saito K, Akutsu K, Takatori S, Ishii R, Hayashi R, Izumi S, Sugino N, Kondo F, Horie M, Nakazawa H, Makino T, Hirosawa M, Shiota K, Ohgane J.  Putative Epimutagens in Maternal Peripheral and Cord Blood Samples Identified Using Human Induced Pluripotent Stem Cells.  Biomed Res Int  2015  2015:876047  PubMed ID: 26339649   DOI: 10.1155/2015/876047
9391  Hideki Sugimoto, Kiyoko Watanabe, Toshizo Toyama, Shun-suke Takahashi, Shuta Sugiyama, Masaichi-Chang-il Lee, Nobushiro Hamada  Inhibitory Effects of French Pine Bark Extract, Pycnogenol®, on Alveolar Bone Resorption and on the Osteoclast Differentiation  Phytother Res  2015  29(2):251-9  PubMed ID: 25336411   DOI: 10.1002/ptr.5245
14984  Himeno T, Kamiya H, Naruse K, Cheng Z, Ito S, Shibata T, Kondo M, Kato J, Okawa T, Fujiya A, Suzuki H, Kito T, Hamada Y, Oiso Y, Isobe K, Nakamura J.  Angioblast Derived from ES Cells Construct Blood Vessels and Ameliorate Diabetic Polyneuropathy in Mice  J Diabetes Res  2015  2015:257230  PubMed ID: 25977928   DOI: 10.1155/2015/257230
15164  An in vitro model of latency and reactivation of varicella zoster virus in human stem cell-derived neurons  An in vitro model of latency and reactivation of varicella zoster virus in human stem cell-derived neurons  PLoS Pathog  2015  11(6):e1004885  PubMed ID: 26042814   DOI: 10.1371/journal.ppat.1004885
15252  Joshi R, Tavana H.  Microengineered embryonic stem cells niche to induce neural differentiation  Annu Int Conf IEEE Eng Med Biol Soc  2015  2015:3557-60  PubMed ID: 26737061   DOI: 10.1109/EMBC.2015.7319161
9867  Takeda O, Toyama T, Watanabe K, Sato T, Sasaguri K, Akimoto S, Sato S, Kawata T, Hamada N.  Ameliorating effects of Juzentaihoto on restraint stress and P. gingivalis-induced alveolar bone loss.  Arch. Oral Biol.  2014  59:1130-8  PubMed ID: 25064760   DOI: 10.1016/j.archoralbio.2014.06.010
5418  Wong CP, Kaneda T, Hadi AH, Morita H.  Ceramicine B, a limonoid with anti-lipid droplets accumulation activity from Chisocheton ceramicus.  J Nat Med  2014  68(1):22-30  PubMed ID: 23494817   DOI: 10.1007/s11418-013-0755-2
9345  Sunao Takeshita, Toshio Fumoto, Yoshinori Naoe, Kyoji Ikeda  Age-related Marrow Adipogenesis Is Linked to Increased Expression of RANKL  J Biol Chem  2014  289(24):16699-710  PubMed ID: 24753250   DOI: 10.1074/jbc.M114.547919
17154  Yang D, Li T, Xu M, Gao F, Yang J, Yang Z, Le W.  Graphene oxide promotes the differentiation of mouse embryonic stem cells to dopamine neurons  Nanomedicine (Lond)  2014  9(16):2445-55  PubMed ID: 24564300   DOI: 10.2217/nnm.13.197
5998  Chan AA, Hertsenberg AJ, Funderburgh ML, Mann MM, Du Y, Davoli KA, Mich-Basso JD, Yang L, Funderburgh JL.  Differentiation of human embryonic stem cells into cells with corneal keratocyte phenotype.  PLoS One  2013  8(2):e56831  PubMed ID: 23437251   DOI: 10.1371/journal.pone.0056831
6173  Belinsky GS, Sirois CL, Rich MT, Short SM, Moore AR, Gilbert SE, Antic SD.  Dopamine receptors in human embryonic stem cell neurodifferentiation.  Stem Cells Dev  2013  22(10):1522-40  PubMed ID: 23286225   DOI: 10.1089/scd.2012.0150
6208  Ogawa Y, Tanaka M, Tanabe M, Suzuki T, Togawa T, Fukushige T, Kanekura T, Sakuraba H, Oishi K.  Impaired neural differentiation of induced pluripotent stem cells generated from a mouse model of Sandhoff disease.  PLoS One  2013  8(1):e55856  PubMed ID: 23383290   DOI: 10.1371/journal.pone.0055856
15785  Chang DJ, Lee N, Park IH, Choi C, Jeon I, Kwon J, Oh SH, Shin DA, Do JT, Lee DR, Lee H, Moon H, Hong KS, Daley GQ, Song J.  Therapeutic potential of human induced pluripotent stem cells in experimental stroke  Cell Transplant  2013  22(8):1427-40  PubMed ID: 23044029   DOI: 10.3727/096368912X657314
18676  Juopperi TA, Kim WR, Chiang CH, Yu H, Margolis RL, Ross CA, Ming GL, Song H.  Astrocytes generated from patient induced pluripotent stem cells recapitulate features of Huntington's disease patient cells  Mol Brain  2012  5:17  PubMed ID: 22613578   DOI: 10.1186/1756-6606-5-17
5993  enham M, Parish CL, Leaw B, Wright J, Reid CA, Petrou S, Dottori M, Thompson LH.  Neurons derived from human embryonic stem cells extend long-distance axonal projections through growth along host white matter tracts after intra-cerebral transplantation.  Front Cell Neurosci  2012  6:11  PubMed ID: 22470319   DOI: 10.3389/fncel.2012.00011
9227  Toshizo Toyama, Kazuo Todoki, Yusuke Takahashi, Kiyoko Watanabe, Shun-suke Takahashi, Shuta Sugiyama, Masaichi-Chang-il Lee, Nobushiro Hamada  Inhibitory Effects of Jixueteng on P. Gingivalis-Induced Bone Loss and Osteoclast Differentiation  Arch Oral Biol  2012  57(11):1529-36  PubMed ID: 22749496   DOI: 10.1016/j.archoralbio.2012.05.011
9238  Takenori Sato, Kiyoko Watanabe, Hidefumi Kumada, Toshizo Toyama, Nobuyuki Tani-Ishii, Nobushiro Hamada  Peptidoglycan of Actinomyces Naeslundii Induces Inflammatory Cytokine Production and Stimulates Osteoclastogenesis in Alveolar Bone Resorption  Arch Oral Biol  2012  57(11):1522-8  PubMed ID: 22939375   DOI: 10.1016/j.archoralbio.2012.07.012
9248  Y Ouji, S Ishizaka, F Nakamura-Uchiyama, M Yoshikawa  In Vitro Differentiation of Mouse Embryonic Stem Cells Into Inner Ear Hair Cell-Like Cells Using Stromal Cell Conditioned Medium  Cell Death Dis  2012  3(5):e314  PubMed ID: 22622133   DOI: 10.1038/cddis.2012.56
9249  Josema Torres, Javier Prieto, Fabrice C Durupt, Simon Broad, Fiona M Watt  Efficient Differentiation of Embryonic Stem Cells Into Mesodermal Precursors by BMP, Retinoic Acid and Notch Signalling  PLoS One  2012  7(4):e36405  PubMed ID: 22558462   DOI: 10.1371/journal.pone.0036405
9250  Takeshi Shimada, Yoshihiro Takai, Kikuko Shinohara, Atsushi Yamasaki, Keiko Tominaga-Yoshino, Akihiko Ogura, Akihiro Toi, Kouji Asano, Norihito Shintani, Atsuko Hayata-Takano, Akemichi Baba, Hitoshi Hashimoto  A Simplified Method to Generate Serotonergic Neurons From Mouse Embryonic Stem and Induced Pluripotent Stem Cells  J Neurochem  2012  122(1):81-93  PubMed ID: 22404309   DOI: 10.1111/j.1471-4159.2012.07724.x
15737  Yang D, Li T, Wang Y, Tang Y, Cui H, Tang Y, Zhang X, Chen D, Shen N, Le W.  miR-132 regulates the differentiation of dopamine neurons by directly targeting Nurr1 expression  J Cell Sci  2012  125(Pt 7):1673-82  PubMed ID: 22328530   DOI: 10.1242/jcs.086421
15997  Nefzger CM, Su CT, Fabb SA, Hartley BJ, Beh SJ, Zeng WR, Haynes JM, Pouton CW.  Lmx1a allows context-specific isolation of progenitors of GABAergic or dopaminergic neurons during neural differentiation of embryonic stem cells  Stem Cells  2012  30(7):1349-61  PubMed ID: 22495882   DOI: 10.1002/stem.1105
7759  Liu CQ, Guo Y, Guan WJ, Ma YH.  Establishment and characterization of a fibroblast cell line derived from Mongolian sheep.  Anim. Sci. J.  2011  82:215-22  PubMed ID: 21729198   DOI: 10.1111/j.1740-0929.2010.00824.x
7841  Markus A, Grigoryan S, Sloutskin A, Yee MB, Zhu H, Yang IH, Thakor NV, Sarid R, Kinchington PR, Goldstein RS.  Varicella-zoster virus (VZV) infection of neurons derived from human embryonic stem cells: direct demonstration of axonal infection, transport of VZV, and productive neuronal infection.  J. Virol.  2011  85:6220-33  PubMed ID: 21525353   DOI: 10.1128/JVI.02396-10
8061  Zhong B, Trobridge GD, Zhang X, Watts KL, Ramakrishnan A, Wohlfahrt M, Adair JE, Kiem HP.  Efficient generation of nonhuman primate induced pluripotent stem cells.  Stem Cells Dev.  2011  20:795-807  PubMed ID: 21058905   DOI: 10.1089/scd.2010.0343
8065  Ziegler L, Grigoryan S, Yang IH, Thakor NV, Goldstein RS.  Efficient generation of schwann cells from human embryonic stem cell-derived neurospheres.  Stem Cell Rev  2011  7:394-403  PubMed ID: 21052870   DOI: 10.1007/s12015-010-9198-2
8107  Kitajima S, Miki T, Takegami Y, Kido Y, Noda M, Hara E, Shamma A, Takahashi C.  Reversion-inducing cysteine-rich protein with Kazal motifs interferes with epidermal growth factor receptor signaling.  Oncogene  2011  30:737-50  PubMed ID: 20890302   DOI: 10.1038/onc.2010.448
8431  Mark Denham, Mirella Dottori  Neural Differentiation of Induced Pluripotent Stem Cells  Methods Mol Biol  2011  793:99-110  PubMed ID: 21913096   DOI: 10.1007/978-1-61779-328-8_7
12232  Jae-Hyung Jang, James T Koerber, Jung-Suk Kim, Prashanth Asuri, Tandis Vazin, Melissa Bartel, Albert Keung, Inchan Kwon, Kook In Park, David V Schaffer  An evolved adeno-associated viral variant enhances gene delivery and gene targeting in neural stem cells  Mol Ther  2011  19(4):667-75  PubMed ID: 21224831   DOI: 10.1038/mt.2010.287
15630  Otani T, Tsuji-Takayama K, Okochi A, Yamamoto M, Takeuchi M, Yamasaki F, Nakamura S, Kibata M.  Stromal cells' B7-1 is a key stimulatory molecule for interleukin-10 production by HOZOT, a multifunctional regulatory T-cell line  Immunol Cell Biol  2011  89(2):246-54  PubMed ID: 20628373   DOI: 10.1038/icb.2010.87
17989  Behavioral improvement after transplantation of neural precursors derived from embryonic stem cells into the globally ischemic brain of adolescent rats  Behavioral improvement after transplantation of neural precursors derived from embryonic stem cells into the globally ischemic brain of adolescent rats  Brain Dev  2010  32(8):658-68  PubMed ID: 19854013   DOI: 10.1016/j.braindev.2009.09.010
8281  Fernando CV, Moses D, Parish CL, Tomas D, Drago J, Horne MK.  Creating a ventral midbrain stem cell niche in an animal model for Parkinson's disease.  Stem Cells Dev.  2010  19:1995-2007  PubMed ID: 20415529   DOI: 10.1089/scd.2009.0486
8480  Ziegler L, Segal-Ruder Y, Coppola G, Reis A, Geschwind D, Fainzilber M, Goldstein RS.  A human neuron injury model for molecular studies of axonal regeneration.  Exp. Neurol.  2010  223:119-27  PubMed ID: 19804775   DOI: 10.1016/j.expneurol.2009.09.019
8521  Yamazoe H, Okuyama T, Suzuki H, Fukuda J.  Fabrication of patterned cell co-cultures on albumin-based substrate: applications for microfluidic devices.  Acta Biomater  2010  6:526-33  PubMed ID: 19654059   DOI: 10.1016/j.actbio.2009.07.036
5779  Takenaka C, Nishishita N, Takada N, Jakt LM, Kawamata S.  Effective generation of iPS cells from CD34+ cord blood cells by inhibition of p53.  Exp Hematol  2010  38(2):154-62  PubMed ID: 19922768   DOI: 10.1016/j.exphem.2009.11.003
5988  Oda Y, Yoshimura Y, Ohnishi H, Tadokoro M, Katsube Y, Sasao M, Kubo Y, Hattori K, Saito S, Horimoto K, Yuba S, Ohgushi H.  Induction of pluripotent stem cells from human third molar mesenchymal stromal cells.  J Biol Chem  2010  285(38):29270-8  PubMed ID: 20595386   DOI: 10.1074/jbc.M109.055889
15629  Goldstein RS, Pomp O, Brokhman I, Ziegler L.  Generation of neural crest cells and peripheral sensory neurons from human embryonic stem cells  Methods Mol Biol  2010  584:283-300  PubMed ID: 19907983   DOI: 10.1007/978-1-60761-369-5_15
18299  Theodorou E, Dalembert G, Heffelfinger C, White E, Weissman S, Corcoran L, Snyder M.  A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation  Genes Dev  2009  23(5):575-88  PubMed ID: 19270158   DOI: 10.1101/gad.1772509
8527  Nishimura K, Nakagawa T, Ono K, Ogita H, Sakamoto T, Yamamoto N, Okita K, Yamanaka S, Ito J.  Transplantation of mouse induced pluripotent stem cells into the cochlea.  Neuroreport  2009  20:1250-4  PubMed ID: 19625987   DOI: 10.1097/WNR.0b013e32832ff287
8719  Ogita H, Nakagawa T, Lee KY, Inaoka T, Okano T, Kikkawa YS, Sakamoto T, Ito J.  Surgical invasiveness of cell transplantation into the guinea pig cochlear modiolus.  ORL J. Otorhinolaryngol. Relat. Spec.  2009  71:32-9  PubMed ID: 18971600   DOI: 10.1159/000165915
5986  Jiang X, Gwye Y, McKeown SJ, Bronner-Fraser M, Lutzko C, Lawlor ER.  Isolation and characterization of neural crest stem cells derived from in vitro-differentiated human embryonic stem cells.  Stem Cells Dev  2009  18(7):1059-70  PubMed ID: 19099373   DOI: 10.1089/scd.2008.0362
5987  Fusaki N, Ban H, Nishiyama A, Saeki K, Hasegawa M.  Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome.  Proc Jpn Acad Ser B Phys Biol Sci  2009  85(8):348-62.  PubMed ID: 19838014   DOI: 10.2183/pjab.85.348
6134  Ban N, Kai M  Implication of replicative stress-related stem cell ageing in radiation-induced murine leukaemia.  Br J Cancer.   2009  101(2):363-71  PubMed ID: 19513063   DOI: 10.1038/sj.bjc.6605135
6169  Vazin 1, Becker KG, Chen J, Spivak CE, Lupica CR, Zhang Y, Worden L, Freed WJ.  A novel combination of factors, termed SPIE, which promotes dopaminergic neuron differentiation from human embryonic stem cells.  PLoS One  2009  4(8):e6606  PubMed ID: 19672298   DOI: 10.1371/journal.pone.0006606
8033  Takahashi J, Takagi Y, Saiki H.  Transplantation of embryonic stem cell-derived dopaminergic neurons in MPTP-treated monkeys  Methods Mol Biol  2009  482:199-212  PubMed ID: 19089358   DOI: 10.1007/978-1-59745-060-7_13
8747  Matsumoto M, Nakagawa T, Kojima K, Sakamoto T, Fujiyama F, Ito J.  Potential of embryonic stem cell-derived neurons for synapse formation with auditory hair cells.  J. Neurosci. Res.  2008  86:3075-85  PubMed ID: 18543340   DOI: 10.1002/jnr.21754
5984  Pomp O, Brokhman I, Ziegler L, Almog M, Korngreen A, Tavian M, Goldstein RS.  PA6-induced human embryonic stem cell-derived neurospheres: a new source of human peripheral sensory neurons and neural crest cells.  Brain Res  2008  1230:50-60  PubMed ID: 18671952   DOI: 10.1016/j.brainres.2008.07.029
5985  Vazin T, Chen J, Lee CT, Amable R, Freed WJ.  Assessment of stromal-derived inducing activity in the generation of dopaminergic neurons from human embryonic stem cells.  Stem Cells  2008  26(6):1517-25  PubMed ID: 18388303   DOI: 10.1634/stemcells.2008-0039
7645  Shimizu N, Noda S, Katayama K, Ichikawa H, Kodama H, Miyoshi H.  Identification of genes potentially involved in supporting hematopoietic stem cell activity of stromal cell line MC3T3-G2/PA6.  Int J Hematol  2008  87(3):239-45  PubMed ID: 18317883   DOI: 10.1007/s12185-008-0048-9
7676  Yagi M, Suzuki N, Takayama T, Arisue M, Kodama T, Yoda Y, Numasaki H, Otsuka K, Ito K.  Lactoferrin suppress the adipogenic differentiation of MC3T3-G2/PA6 cells.  J Oral Sci  2008  50(4):419-25  PubMed ID: 19106469   DOI: 10.2334/josnusd.50.419
9485  Tandis Vazin, Jia Chen, Charles E Spivak, Rose Amable, Emily Gabitzsch, Chun-Ting Lee, Carl R Lupica, William J Freed  Dopaminergic Neurons Derived From BG01V2, a Variant of Human Embryonic Stem Cell Line BG01  Restor Neurol Neurosci   2008  26(6):447-58  PubMed ID: 19096132  
5975  Hedlund E, Pruszak J, Ferree A, Viñuela A, Hong S, Isacson O, Kim KS.  Selection of embryonic stem cell-derived enhanced green fluorescent protein-positive dopamine neurons using the tyrosine hydroxylase promoter is confounded by reporter gene expression in immature cell populations.  Stem Cells  2007  25(5):1126-35  PubMed ID: 17234989   DOI: 10.1634/stemcells.2006-0540
7353  Anisimov SV, Christophersen NS, Correia AS, Li JY, Brundin P.  "NeuroStem Chip": a novel highly specialized tool to study neural differentiation pathways in human stem cells.  BMC Genomics  2007  8:46  PubMed ID: 17288595   DOI: 10.1186/1471-2164-8-46
7354  Takahashi N, Udagawa N, Kobayashi Y, Suda T.  Generation of osteoclasts in vitro, and assay of osteoclast activity.  Methods Mol Med  2007  135:285-301  PubMed ID: 17951666   DOI: 10.1007/978-1-59745-401-8_18
8942  Kazutoshi Takahashi, Koji Tanabe, Mari Ohnuki, Megumi Narita, Tomoko Ichisaka, Kiichiro Tomoda, Shinya Yamanaka  Induction of Pluripotent Stem Cells From Adult Human Fibroblasts by Defined Factors  Cell  2007  131 (5), 861-72  PubMed ID: 18035408   DOI: 10.1016/j.cell.2007.11.019
3399  Moriyasu, Kenta, Yamazoe, Hironori, Iwata, Hiroo  Induction dopamine releasing cells from mouse embryonic stem cells and their long-term culture.  J Biomed Mater Res A  2006  77:136-47  PubMed ID: 16392122   DOI: 10.1002/jbm.a.30576
8863  Sato N, Brivanlou AH.  Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor.  Methods Mol. Biol.  2006  331:115-28  PubMed ID: 16881513   DOI: 10.1385/1-59745-046-4:115
8921  Sekiya T, Kojima K, Matsumoto M, Kim TS, Tamura T, Ito J.  Cell transplantation to the auditory nerve and cochlear duct.  Exp. Neurol.  2006  198:12-24  PubMed ID: 16376874   DOI: 10.1016/j.expneurol.2005.11.006
5974  Lin L1, Isacson O.  Axonal growth regulation of fetal and embryonic stem cell-derived dopaminergic neurons by Netrin-1 and Slits.  Stem Cells  2006  24(11):2504-13  PubMed ID: 16840550   DOI: 10.1634/stemcells.2006-0119
5981  Carpenter M, Rao MS, Freed W, Zeng X.  Derivation and characterization of neuronal precursors and dopaminergic neurons from human embryonic stem cells in vitro.  Methods Mol Biol  2006  331:153-67.  PubMed ID: 16881516   DOI: 10.1385/1-59745-046-4:153
6149  Roybon L, Ma Z, Asztely F, Fosum A, Jacobsen SE, Brundin P, Li JY.  Failure of transdifferentiation of adult hematopoietic stem cells into neurons.  Stem Cells  2006  24(6):1594-604  PubMed ID: 16556707   DOI: 10.1634/stemcells.2005-0548
6984  Takahashi M, Haruta M.  Derivation and characterization of lentoid bodies and retinal pigment epithelial cells from monkey embryonic stem cells in vitro.  Methods Mol Biol  2006  330:417-29  PubMed ID: 16846040   DOI: 10.1385/1-59745-036-7:417
2925  Thinyane K, Baier PC, Schindehutte J, Mansouri A, Paulus W, Trenkwalder C, Flugge G, Fuchs E  Fate of pre-differentiated mouse embryonic stem cells transplanted in unilaterally 6-hydroxydopamine lesioned rats: histological characterization of the grafted cells.  Brain Res  2005  1045:80-7  PubMed ID: 15910765   DOI: 10.1016/j.brainres.2005.03.033
3373  Pomp, Oz, Brokhman, Irina, Ben-Dor, Israel, Reubinoff, Benjamin, Goldstein, Ronald S  Generation of peripheral sensory and sympathetic neurons and neural crest cells from human embryonic stem cells.  Stem Cells  2005  23:923-30  PubMed ID: 15883233   DOI: 10.1634/stemcells.2005-0038
8938  Okano T, Nakagawa T, Endo T, Kim TS, Kita T, Tamura T, Matsumoto M, Ohno T, Sakamoto T, Iguchi F, Ito J.  Engraftment of embryonic stem cell-derived neurons into the cochlear modiolus.  Neuroreport  2005  16:1919-22  PubMed ID: 16272879   DOI: 10.1097/01.wnr.0000187628.38010.5b
8972  Kim TS, Nakagawa T, Kita T, Higashi T, Takebayashi S, Matsumoto M, Kojima K, Sakamoto T, Ito J.  Neural connections between embryonic stem cell-derived neurons and vestibular hair cells in vitro.  Brain Res.  2005  1057:127-33  PubMed ID: 16122715   DOI: 10.1016/j.brainres.2005.07.059
9025  Matsumoto M, Nakagawa T, Higashi T, Kim TS, Kojima K, Kita T, Sakamoto T, Ito J.  Innervation of stem cell-derived neurons into auditory epithelia of mice.  Neuroreport  2005  16:787-90  PubMed ID: 15891570   DOI: 10.1097/00001756-200505310-00001
6828  Morito S, Yaguchi K, Imada M, Tachikawa C, Nomura M, Moritani S, Igarashi M, Yokogawa K, Miyamoto K.  Insulin signaling in adipocytes differentiated from mouse stromal MC3T3-G2/PA6 cells.  Biol Pharm Bull  2005  28(11):2040-5  PubMed ID: 16272686   DOI: 10.1248/bpb.28.2040
15628  Roybon L, Brundin P, Li JY.  Stromal cell-derived inducing activity does not promote dopaminergic differentiation, but enhances differentiation and proliferation of neural stem cell-derived astrocytes  Exp Neurol  2005  196(2):373-80  PubMed ID: 16199035   DOI: 10.1016/j.expneurol.2005.08.023
3158  Nishimura, Haruki, Nishimura, Masahiro, Oda, Ryo, Yamanaka, Katsuyuki, Matsubara, Takehiro, Ozaki, Yoshie, Sekiya, Kensuke, Hamada, Taizo, Kato, Yukio  Lectins induce resistance to proteases and/or mechanical stimulus in all examined cells--including bone marrow mesenchymal stem cells--on various scaffolds.  Exp Cell Res  2004  295:119-27  PubMed ID: 15051495   DOI: 10.1016/j.yexcr.2003.12.018
3359  Baier, Paul Christian, Schindehutte, Jan, Thinyane, Keneuoe, Flugge, Gabriele, Fuchs, Eberhard, Mansouri, Ahmed, Paulus, Walter, Gruss, Peter, Trenkwalder, Claudia  Behavioral changes in unilaterally 6-hydroxy-dopamine lesioned rats after transplantation of differentiated mouse embryonic stem cells without morphological integration.  Stem Cells  2004  22:396-404  PubMed ID: 15153616   DOI: 10.1634/stemcells.22-3-396
8592  Tatsunori Sakamoto, Takayuki Nakagawa, Tsuyoshi Endo, Tae-Soo Kim, Fukuichiro Iguchi, Yasushi Naito, Yoshiki Sasai, Juichi Ito  Fates of Mouse Embryonic Stem Cells Transplanted Into the Inner Ears of Adult Mice and Embryonic Chickens  Acta Otolaryngol  2004  Suppl 551, 48-5  PubMed ID: 15078078   DOI: 10.1080/03655230310016825
16814  T Sugimoto, A Ide-Ektessabi, R Ishihara, M Tanigaki  An investigation of changes in element distribution and chemical states during differentiation of embryonic stem cells  Journal of Electron Spectroscopy and Related Phenomena  2004  137-140:831-838    DOI: 10.1016/j.elspec.2004.02.151
6629  Takahashi N, Udagawa N, Tanaka S, Suda T.  Generating murine osteoclasts from bone marrow.  Methods Mol Med  2003  80:129-44  PubMed ID: 12728715   DOI: 10.1385/1-59259-366-6:129
6634  Hiramine H, Watanabe K, Hamada N, Umemoto T.  Porphyromonas gingivalis 67-kDa fimbriae induced cytokine production and osteoclast differentiation utilizing TLR2.  FEMS Microbiol Lett  2003  229(1):49-55  PubMed ID: 14659542   DOI: 10.1016/S0378-1097(03)00788-2
15627  Barberi T, Klivenyi P, Calingasan NY, Lee H, Kawamata H, Loonam K, Perrier AL, Bruses J, Rubio ME, Topf N, Tabar V, Harrison NL, Beal MF, Moore MA, Studer L.  Neural subtype specification of fertilization and nuclear transfer embryonic stem cells and application in parkinsonian mice  Nat Biotechnol  2003  21(10):1200-7  PubMed ID: 14502203   DOI: 10.1038/nbt870
16305  Nagano M, Ryu BY, Brinster CJ, Avarbock MR, Brinster RL.  Maintenance of mouse male germ line stem cells in vitro  Biol Reprod  2003  68(6):2207-14  PubMed ID: 12606373   DOI: 10.1095/biolreprod.102.014050
15537  Wichterle H, Lieberam I, Porter JA, Jessell TM.  Directed differentiation of embryonic stem cells into motor neurons  Cell   2002  110(3):385-97  PubMed ID: 12176325   DOI: 10.1016/s0092-8674(02)00835-8
3990  Takami M, Takahashi N, Udagawa N, Miyaura C, Suda K, Woo JT, Martin TJ, Nagai K, Suda T.  Intracellular calcium and protein kinase C mediate expression of receptor activator of nuclear factor-kappaB ligand and osteoprotegerin in osteoblasts.  Endocrinology  2000  141(12):4711-9  PubMed ID: 11108286   DOI: 10.1210/endo.141.12.7852
3960  Fujiwara M, Tagashira S, Harada H, Ogawa S, Katsumata T, Nakatsuka M, Komori T, Takada H.  Isolation and characterization of the distal promoter region of mouse Cbfa1.  Biochim Biophys Acta  1999  1446(3):265-72  PubMed ID: 10524201   DOI: 10.1016/s0167-4781(99)00113-x
3964  Nagai M, Sato N.  Reciprocal gene expression of osteoclastogenesis inhibitory factor and osteoclast differentiation factor regulates osteoclast formation.  Biochem Biophys Res Commun  1999  257(3):719-23  PubMed ID: 10208850   DOI: 10.1006/bbrc.1999.0524
2274  Nishikawa M, Yamamoto M, Murakami T, Akatsu T, Kugai N, Nagata N  A third-generation bisphosphonate, YM175, inhibits osteoclast formation in murine cocultures by inhibiting proliferation of precursor cells via supporting cell-dependent mechanisms.  J Bone Miner Res  1998  13:986-95  PubMed ID: 9626630   DOI: 10.1359/jbmr.1998.13.6.986
15406  Mio H, Kagami N, Yokokawa S, Kawai H, Nakagawa S, Takeuchi K, Sekine S, Hiraoka A.  Isolation and characterization of a cDNA for human mouse, and rat full-length stem cell growth factor, a new member of C-type lectin superfamily  Biochem Biophys Res Commun  1998  249(1):124-30  PubMed ID: 9705843   DOI: 10.1006/bbrc.1998.9073
15407  Suzu S, Hatake K, Ota J, Mishima Y, Yamada M, Shimamura S, Kimura F, Motoyoshi K.  Identification of alternatively spliced transcripts encoding murine macrophage colony-stimulating factor  Biochem Biophys Res Commun  1998  245(1):120-6  PubMed ID: 9535794   DOI: 10.1006/bbrc.1998.8394
3938  Tsuda E, Goto M, Mochizuki S, Yano K, Kobayashi F, Morinaga T, Higashio K.  Isolation of a novel cytokine from human fibroblasts that specifically inhibits osteoclastogenesis.  Biochem Biophys Res Commun  1997  234(1):137-42  PubMed ID: 9168977   DOI: 10.1006/bbrc.1997.6603
3927  Yamaguchi 1, Ishizuya T, Kintou N, Wada Y, Katagiri T, Wozney JM, Rosen V, Yoshiki S.  Effects of BMP-2, BMP-4, and BMP-6 on osteoblastic differentiation of bone marrow-derived stromal cell lines, ST2 and MC3T3-G2/PA6.  Biochem Biophys Res Commun  1996  220(2):366-71  PubMed ID: 8645311   DOI: 10.1006/bbrc.1996.0411

Back Back Return Top Page