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Cell No. : Cell Name
RCB1125 : TOM-2  update : 2022/05/30
CommentGlassy cell carcinoma from cervix.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
University of Tsukuba

1-1-1 Tenno-dai, Tsukuba-Shi, Ibaraki-ken, 305-8575 Japan
Dr.OKI Akinori
Fax. +81-29-853-3072
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Oki, Akinori
Originator Oki, Akinori
Year of deposit 1994
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 27 years
Tissue uterus, lymph node meta
Disease name glassycell carcinoma
Metastatic ability Yes
Metastatic tissue lymph node
Classification cancer
Year of origin 1993
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_5603
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method (0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 74-82(50) : /75(6),76(11),77(14),78(4),79(9)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
2288  Oki A, Nishida M, Satoh T, Tsunoda H, Kasahara K, Saijo K, Kubo T, Ohno T  A novel human glassy-cell carcinoma cell line producing IL-6 and IL-8 from uterine cervix.  In Vitro Cell Dev Biol Anim  1998  34:290-7  PubMed ID: 9590502   DOI: 10.1007/s11626-998-0005-1

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User's Publication
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x


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