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細胞番号 : 細胞名
RCB1124 : OP9  update : 2021/04/28
細胞特性(Comment:英)Support hematpoietic stem cell and ES cell differentiation to blood cells and lymphocytes.
細胞特性(日)大理石骨病を発症するop/opマウス由来で、M-CSF遺伝子に突然変異を持つ。脂肪細胞に分化し、造血幹細胞の増殖、ES細胞から血球系、リンパ球系への細胞分化を支持する機能を持っている。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)Basically, there is no restriction regarding academic use. For commercial use or patent application, please contact Rebirthel Co., Ltd. (info@rebirthel.com).
使用条件(日)学術研究における利用につきましては原則として使用制限はありません。特許等の取得及び商業用途(商業利用)の使用については、リバーセル株式会社(info@rebirthel.com)に問い合わせてください。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず(cellbank.brc@riken.jp)までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Kodama, H. & Nakano, T.
樹立者 Kodama, Hiroaki
寄託日 1994
動物種 _mouse < Mammals
動物系統名 B6C3Fe-a/a, op
性別 Unknown
0 day
採取組織 newborn, calvaria
遺伝的特徴 op/op
細胞分類 other
細胞寿命 infinite
細胞形態 fibroblast-like
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 MEM ALPHA + 20% FBS
抗生物質 Free
継代方法 0.25% trypsin
継代密度 1 : 2-4 split
継代・培地交換頻度 Subculture or Medium Renewal : once/2 days
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
動物種PCR OK
マウス系統検査 OK
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 0件
Reference(日) 0件
利用者成果(英) 49件
利用者成果(日) 1件

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Reference(英)

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Reference(日)

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利用者成果(英)
20033  Nita A, Muto Y, Katayama Y, Matsumoto A, Nishiyama M, Nakayama KI.  The autism-related protein CHD8 contributes to the stemness and differentiation of mouse hematopoietic stem cells.  Cell Rep  2021  34(5):108688  PubMed ID: 33535054
20079  Miyoshi H, Abo K, Hosoya D, Matsuo K, Utsumi Y.  Effects of mouse fetal liver cell culture density on hematopoietic cell expansion in three-dimensional cocultures with stromal cells.  Int J Artif Organs  2021  391398821996377  PubMed ID: 33611956
20107  Jia W, Kong L, Kidoya H, Naito H, Muramatsu F, Hayashi Y, Hsieh HY, Yamakawa D, Hsu DK, Liu FT, Takakura N.  Indispensable role of Galectin-3 in promoting quiescence of hematopoietic stem cells.  Nat Commun  2021  12(1):2118  PubMed ID: 33837181
20121  Xiao L, Miwa N.  Hydrogen Nano-Bubble Water Suppresses ROS Generation, Adipogenesis, and Interleukin-6 Secretion in Hydrogen-Peroxide- or PMA-Stimulated Adipocytes and Three-Dimensional Subcutaneous Adipose Equivalents.  Cells  2021  10(3)  PubMed ID: 33799840
10005  Soki Kashima, Takuya Maeda, Kyoko Masuda, Seiji Nagano, Takamitsu Inoue, Masashi Takeda, Yuka Kono, Takashi Kobayashi, Shigeyoshi Saito, Takahiro Higuchi, Hiroshi Ichise, Yuka Kobayashi, Keiko Iwaisako, Koji Terada, Yasutoshi Agata, Kazuyuki Numakura, Mitsuru Saito, Shintaro Narita, Masaki Yasukawa, Osamu Ogawa, Tomonori Habuchi, Hiroshi Kawamoto  Cytotoxic T Lymphocytes Regenerated From iPS Cells Have Therapeutic Efficacy in a Patient-Derived Xenograft Solid Tumor Model  iScience  2020  23(4):100998  PubMed ID: 32259478
11925  Wakao H.  Reprogramming of MAIT Cells to Pluripotency and Redifferentiation.  Methods Mol. Biol.  2020    PubMed ID: 31792827
12032  Naito H, Wakabayashi T, Ishida M, Gil CH, Iba T, Rahmawati FN, Shimizu S, Yoder MC, Takakura N.  Isolation of tissue-resident vascular endothelial stem cells from mouse liver.  Nat Protoc  2020    PubMed ID: 32005982
12168  Kana Kuronuma, Aya Yokoi, Tomoya Fukuoka, Muneaki Miyata, Akio Maekawa, Satowa Tanaka, Leo Matsubara, Chie Goto, Miki Matsuo, Hao-Wei Han, Mai Tsuruta, Haruka Murata, Hikari Okamoto, Natsumi Hasegawa, Shigetaka Asano, Mitsuhiro Ito  Matrix Gla protein maintains normal and malignant hematopoietic progenitor cells by interacting with bone morphogenetic protein-4  Heliyon  2020    PubMed ID: 32322728
4047  Naito H, Iba T, Wakabayashi T, Tai-Nagara I, Suehiro JI, Jia W, Eino D, Sakimoto S, Muramatsu F, Kidoya H, Sakurai H, Satoh T, Akira S, Kubota Y, Takakura N.  TAK1 Prevents Endothelial Apoptosis and Maintains Vascular Integrity  Dev Cell  2019  48(2):151-166  PubMed ID: 30639056
11544  Iba T, Naito H, Shimizu S, Rahmawati FN, Wakabayashi T, Takakura N.  Isolation of tissue-resident endothelial stem cells and their use in regenerative medicine.  Inflamm Regen  2019    PubMed ID: 31086611
12535  Tsukamoto T.  HIV Impacts CD34 + Progenitors Involved in T-Cell Differentiation During Coculture With Mouse Stromal OP9-DL1 Cells  Front Immunol  2019  10:81  PubMed ID: 30761146
7000  Wakabayashi T, Naito H, Suehiro JI, Lin Y, Kawaji H, Iba T, Kouno T, Ishikawa-Kato S, Furuno M, Takara K, Muramatsu F, Weizhen J, Kidoya H, Ishihara K, Hayashizaki Y, Nishida K, Yoder MC, Takakura N.  CD157 Marks Tissue-Resident Endothelial Stem Cells with Homeostatic and Regenerative Properties.  Cell Stem Cell  2018  22(3):384-397  PubMed ID: 29429943
10826  Tadokoro Y, Hoshii T, Yamazaki S, Eto K, Ema H, Kobayashi M, Ueno M, Ohta K, Arai Y, Hara E, Harada K, Oshima M, Oshima H, Arai F, Yoshimura A, Nakauchi H, Hirao A.  Spred1 Safeguards Hematopoietic Homeostasis against Diet-Induced Systemic Stress.  Cell Stem Cell  2018  22:713-725.e8  PubMed ID: 29706577
10294  Nishimura T, Unezaki N, Kanegi R, Wijesekera DPH, Hatoya S, Sugiura K, Kawate N, Tamada H, Imai H, Inaba T.  Generation of Canine Induced Extraembryonic Endoderm-Like Cell Line That Forms Both Extraembryonic and Embryonic Endoderm Derivatives.  Stem Cells Dev.  2017  26:1111-1120  PubMed ID: 28474540
7053  Maeda T, Nagano S, Ichise H, Kataoka K, Yamada D, Ogawa S, Koseki H, Kitawaki T, Kadowaki N, Takaori-Kondo A, Masuda K, Kawamoto H.  Regeneration of CD8αβ T Cells from T-cell-Derived iPSC Imparts Potent Tumor Antigen-Specific Cytotoxicity.  Cancer Res.  2016  76:6839-6850  PubMed ID: 27872100
10017  Tanaka S, Maekawa A, Matsubara L, Imanishi A, Yano M, Roeder RG, Hasegawa N, Asano S, Ito M.  Periostin supports hematopoietic progenitor cells and niche-dependent myeloblastoma cells in vitro.  Biochem. Biophys. Res. Commun.  2016  478:1706-12  PubMed ID: 27596966
4000  Okada A, Tashiro K, Yamaguchi T, Kawabata K.  Selective Differentiation into Hematopoietic and Cardiac Cells from Pluripotent Stem Cells Based on the Expression of Cell Surface Markers.  Methods Mol Biol  2016  1341:181-95  PubMed ID: 26138986
7479  Kato S, Kato Y, Shibata H, Saitoh Y, Miwa N.  Repressive effects of oat extracts on intracellular lipid-droplet formation in adipocytes and a three-dimensional subcutaneous adipose tissue model.  Mater Sci Eng C Mater Biol Appl  2015  49:269-73  PubMed ID: 25686949
7573  Yoshimitsu M, Higuchi K, Arima N, Medin JA, Takenaka T  Successful Induction of Pluripotent Stem Cells From a Fabry Disease Mouse Model: Toward the Development of Safe Lentiviral Gene Therapy.  J Stem Cell Res Ther  2015  5:1000259 
3948  Tashiro K, Hirata N, Okada A, Yamaguchi T, Takayama K, Mizuguchi H, Kawabata K.  Expression of coxsackievirus and adenovirus receptor separates hematopoietic and cardiac progenitor cells in fetal liver kinase 1-expressing mesoderm.  Stem Cells Transl Med  2015  4(5):424-36  PubMed ID: 25762001
5454  Kudo H, Wada H, Sasaki H, Tsuji H, Otsuka R, Baghdadi M, Kojo S, Chikaraishi T, Seino K.  Induction of macrophage-like immunosuppressive cells from mouse ES cells that contribute to prolong allogeneic graft survival.  PLoS One.  2014  9(10):e111826  PubMed ID: 25356669
6066  Awong G, Zúñiga-Pflücker JC.  Generation, isolation, and engraftment of in vitro-derived human T cell progenitors.  Methods Mol Biol  2013  946:103-13  PubMed ID: 23179828
6084  Kato S, Saitoh Y, Miwa N.  Repressive effects of a capacitive-resistive electric transfer (CRet) hyperthermic apparatus combined with provitamin C on intracellular lipid-droplets formation in adipocytes.  Int J Hyperthermia  2013  29(1):30-7  PubMed ID: 23286696
9011  Ryo Kurita, Noriko Suda, Kazuhiro Sudo, Kenichi Miharada, Takashi Hiroyama, Hiroyuki Miyoshi, Kenzaburo Tani, Yukio Nakamura  Establishment of Immortalized Human Erythroid Progenitor Cell Lines Able to Produce Enucleated Red Blood Cells  PLoS One  2013  8 (3), e59890  PubMed ID: 23533656
9363  Ruri Ishino, Kaori Minami, Satowa Tanaka, Mami Nagai, Keiji Matsui, Natsumi Hasegawa, Robert G Roeder, Shigetaka Asano, Mitsuhiro Ito  FGF7 Supports Hematopoietic Stem and Progenitor Cells and Niche-Dependent Myeloblastoma Cells via Autocrine Action on Bone Marrow Stromal Cells in Vitro  Biochem Biophys Res Commun  2013  440(1):125-31  PubMed ID: 24051090
13963  Mohtashami M, Shah DK, Kianizad K, Awong G, Zúñiga-Pflücker JC.  Induction of T-cell development by Delta-like 4-expressing fibroblasts  Int Immunol   2013  25(10):601-11  PubMed ID: 23988616
9142  Divya K Shah, Mahmood Mohtashami, Juan Carlos Zúñiga-Pflücker  Role of Recycling, Mindbomb1 Association, and Exclusion From Lipid Rafts of δ-Like 4 for Effective Notch Signaling to Drive T Cell Development  J Immunol  2012  189 (12), 5797-808  PubMed ID: 23162128
8040  Nishimura K, Sano M, Ohtaka M, Furuta B, Umemura Y, Nakajima Y, Ikehara Y, Kobayashi T, Segawa H, Takayasu S, Sato H, Motomura K, Uchida E, Kanayasu-Toyoda T, Asashima M, Nakauchi H, Yamaguchi T, Nakanishi M.  Development of defective and persistent Sendai virus vector: a unique gene delivery/expression system ideal for cell reprogramming.  J. Biol. Chem.  2011  286:4760-71  PubMed ID: 21138846
8154  Sumitomo A, Ishino R, Urahama N, Inoue K, Yonezawa K, Hasegawa N, Horie O, Matsuoka H, Kondo T, Roeder RG, Ito M.  The transcriptional mediator subunit MED1/TRAP220 in stromal cells is involved in hematopoietic stem/progenitor cell support through osteopontin expression.  Mol. Cell. Biol.  2010  30:4818-27  PubMed ID: 20713445
8233  Mohtashami M, Shah DK, Nakase H, Kianizad K, Petrie HT, Zuniga-Pflucker JC.  Direct comparison of Dll1- and Dll4-mediated Notch activation levels shows differential lymphomyeloid lineage commitment outcomes.  J. Immunol.  2010  185:867-76  PubMed ID: 20548034
8250  Xiao L, Aoshima H, Saitoh Y, Miwa N.  The effect of squalane-dissolved fullerene-C60 on adipogenesis-accompanied oxidative stress and macrophage activation in a preadipocyte-monocyte co-culture system.  Biomaterials  2010  31:5976-85  PubMed ID: 20488530
8423  Suzuki N, Itou T, Hasegawa Y, Okazaki T, Ikeno M.  Cell to cell transfer of the chromatin-packaged human beta-globin gene cluster.  Nucleic Acids Res.  2010  38:e33  PubMed ID: 20007595
6130  Holmes R, Zúñiga-Pflücker JC.  The OP9-DL1 system: generation of T-lymphocytes from embryonic or hematopoietic stem cells in vitro.  Cold Spring Harb Protoc. 2009 Feb  2009  2009(2):pdb.prot5156  PubMed ID: 20147086
12195  Taro Ishigaki, Kazuhiro Sudo, Takashi Hiroyama, Kenichi Miharada, Haruhiko Ninomiya, Shigeru Chiba, Toshiro Nagasawa, Yukio Nakamura  Human hematopoietic stem cells can survive in vitro for several months  Adv Hematol  2009  2009:936761  PubMed ID: 19960048
8722  Nagao K, Ohta T, Hinohara A, Tahara T, Hagiwara T, Maeda Y, Yoneya T, Sohma Y, Heike T, Nakahata T, Inagaki Y, Nishikawa M.  Expression profile analysis of aorta-gonad-mesonephros region-derived stromal cells reveals genes that regulate hematopoiesis.  Biochem. Biophys. Res. Commun.  2008  377:205-9  PubMed ID: 18840400
6137  Awong G1, La Motte-Mohs RN, Zúñiga-Pflücker JC.  In vitro human T cell development directed by notch-ligand interactions.  Methods Mol Biol  2008  430:135-42  PubMed ID: 18370296
7645  Shimizu N, Noda S, Katayama K, Ichikawa H, Kodama H, Miyoshi H.  Identification of genes potentially involved in supporting hematopoietic stem cell activity of stromal cell line MC3T3-G2/PA6.  Int J Hematol  2008  87(3):239-45  PubMed ID: 18317883
10372  Hiroyama T, Miharada K, Sudo K, Danjo I, Aoki N, Nakamura Y.  Establishment of mouse embryonic stem cell-derived erythroid progenitor cell lines able to produce functional red blood cells.  PLoS ONE  2008  3(2):e1544  PubMed ID: 18253492
5989  Tanaka Y, Era T, Nishikawa S, Kawamata S.  Forced expression of Nanog in hematopoietic stem cells results in a gammadeltaT-cell disorder.  Blood  2007  110(1):107-15  PubMed ID: 17360937
7385  Murakami Y, Hirata H, Miyamoto Y, Nagahashi A, Sawa Y, Jakt M, Asahara T, Kawamata S.  Isolation of cardiac cells from E8.5 yolk sac by ALCAM (CD166) expression.  Mech Dev  2007  124(11-12):830-9  PubMed ID: 17964124
10362  Nooshin Tabatabaei-Zavareh, Anastasia Vlasova, Chelsea Pamela Greenwood, Fumio Takei  Characterization of Developmental Pathway of Natural Killer Cells From Embryonic Stem Cells in Vitro  PLoS One  2007  2(2):e232  PubMed ID: 17311098
3411  de Pooter, Renee F, Schmitt, Thomas M, de la Pompa, Jose Luis, Fujiwara, Yuko, Orkin, Stuart H, Zuniga-Pflucker, Juan Carlos  Notch signaling requires GATA-2 to inhibit myelopoiesis from embryonic stem cells and primary hemopoietic progenitors.  J Immunol  2006  176:5267-75  PubMed ID: 16621992
3357  Hiroyama, Takashi, Miharada, Kenichi, Aoki, Naoko, Fujioka, Tsuyoshi, Sudo, Kazuhiro, Danjo, Inaho, Nagasawa, Toshiro, Nakamura, Yukio  Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells.  Exp Hematol  2006  34:760-9  PubMed ID: 16728281
3358  Takayanagi, Shin-ichiro, Hiroyama, Takashi, Yamazaki, Satoshi, Nakajima, Tomoko, Morita, Yohei, Usui, Joichi, Eto, Koji, Motohashi, Tsutomu, Shiomi, Kensuke, Keino-Masu, Kazuko, Masu, Masayuki, Oike, Yuichi, Mori, Shigeo, Yoshida, Nobuaki, Iwama, Atsushi, Nakauchi, Hiromitsu  Genetic marking of hematopoietic stem and endothelial cells: identification of the Tmtsp gene encoding a novel cell-surface protein with the thrombospondin-1 domain.  Blood  2006  107:4317-25  PubMed ID: 16455951
3171  Niida, Shumpei, Kondo, Takako, Hiratsuka, Sachie, Hayashi, Shin-Ichi, Amizuka, Norio, Noda, Tetsuo, Ikeda, Kyoji, Shibuya, Masabumi  VEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice.  Proc Natl Acad Sci U S A  2005  102:14016-21  PubMed ID: 16172397
2666  Kawamura, Toshihiko, Koka, Rima, Ma, Averil, Kumar, Vinay  Differential roles for IL-15R alpha-chain in NK cell development and Ly-49 induction.  J Immunol  2003  171:5085-90  PubMed ID: 14607906
12264  Shintaro Yamazaki, Kenji Miki, Kiyoshi Hasegawa, Masataka Sata, Tadatoshi Takayama, Masatoshi Makuuchi  Sera from liver failure patients and a demethylating agent stimulate transdifferentiation of murine bone marrow cells into hepatocytes in coculture with nonparenchymal liver cells  J Hepatol  2003  39(1):17-23  PubMed ID: 12821039
2428  Okada S, Yoshida T, Hong Z, Ishii G, Hatano M, Kuro-O M, Nabeshima Y, Nabeshima Y, Tokuhisa T  Impairment of B lymphopoiesis in precocious aging (klotho) mice.  Int Immunol  2000  12:861-71  PubMed ID: 10837414
9954  Morgan BJ, Crabtree D, Skatrud JB.  Chemoreflex sensitization augments sympathetic vasomotor outflow in awake humans.  Adv. Exp. Med. Biol.  1994  360:269-71  PubMed ID: 7872100

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利用者成果(日)
3320  石垣太郎、寛山隆、三原田賢一、藤岡剛、須藤和寛、檀上稲穂、長澤俊郎、中村幸夫  臍帯血培養系における Feeder細胞(OP9,10T1/2)の比較  第28回日本分子生物学会  2005   



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