Cell No. : Cell Name
RCB1119 : ψCRE-MFGtsT
update : 2023/08/08
|
| Comment | Cartagena Protocol on Biosafety.High-titer producer of MoMLV-derived retrovirus including SV40 large T temperature-sensitive cDNA. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Hamada, Hirofumi
|
| Originator |
Hamada, Hirofumi
|
| Year of deposit |
1994
|
| Animal |
_mouse
< Mammals
|
| Strain name |
NIH Swiss
|
| Genus |
Mus musculus
|
| Species |
mus domesticus
|
| Age at sampling |
embryo
|
| Tissue |
embryo
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
SV40 large T temperature-sensitive cDNA
|
| Vector |
MFG
|
| Recombinant virus |
Yes
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| BSL |
BSL2
|
| Cellosaurus(Expasy) |
CVCL_U114
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% CS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8-10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Reference information |
Reference |
0
|
| User's Publication |
0
|
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