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Cell No. : Cell Name
RCB1117 : ψCRE-MFGts-c-src  update : 2022/03/29
CommentProducer of MoMLV-derived retrovirus including mouse c-src cDNA with ts mutation.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hamada, Hirofumi
Originator Hamada, Hirofumi
Year of deposit 1994
Animal mouse < Mammals
Strain name NIH Swiss
Genus Mus musculus
Species mus domesticus
Age at sampling embryo
Tissue embryo
Classification transformed
Recombinant recombinant
Exogene ts, mouse c-src cDNA
Vector MFG
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T377
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 0
User's Publication 0


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