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Cell No. : Cell Name
RCB1099 : ψCRIP-MFGmIL-12p35  update : 2022/03/28
CommentCell line producing MoMLV-derived retrovirus to express mouse IL-12 (p35 subunit). Refer to RCB1098. Long term culture results in decrease of virus production.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hamada, Hirofumi
Originator Hamada, Hirofumi
Year of deposit 1994
Original cell ψCRIP
Animal mouse < Mammals
Strain name NIH Swiss
Genus Mus musculus
Species mus domesticus
Age at sampling embryo
Tissue embryo
Classification transformed
Recombinant recombinant
Exogene Amp^r, Mouse IL-12p35 cDNA
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T396
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 4
User's Publication 0


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Reference
2530  Ding LH, Iimura E, Saijo K, Hamada H, Ohno T.  A speedy method to detect inserted DNA fragment in cell lines transfected with retroviral vectors.  Cytotechnology  2000  34(3):243-52  PubMed ID: 19003400   DOI: 10.1023/A:1008187310534
3686  Wakimoto H, Abe J, Tsunoda R, Aoyagi M, Hirakawa K, Hamada H.  Intensified antitumor immunity by a cancer vaccine that produces granulocyte-macrophage colony-stimulating factor plus interleukin 4.  Cancer Res  1996  56(8):1828-33  PubMed ID: 8620500  
2174  Dranoff G, Jaffee E, Lazenby A, Golumbek P, Levitsky H, Brose K, Jackson V, Hamada H, Pardoll D, Mulligan, R C  Vaccination with irradiated tumor cells engineered to secrete murine granulocyte-macrophage colony-stimulating factor stimulates potent, specific, and long-lasting anti-tumor immunity.  Proc Natl Acad Sci U S A  1993  90:3539-43  PubMed ID: 8097319  
2172  Danos O, Mulligan, R C  Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges.  Proc Natl Acad Sci U S A  1988  85:6460-4  PubMed ID: 3413107  

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