Cell No. : Cell Name RCB1062 : LMSU  update : 2024/07/05
Comment			Human cell line derived from lymph node metastasis of gastric cancer.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Okabe, Tetsuro
		Originator	Okabe, Tetsuro
		Year of deposit	1994
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	27 years
		Tissue	stomach, lymph node meta
		Disease name	sarcoma
		Metastatic ability	Yes
		Metastatic tissue	lymph node
		Classification	cancer
		Memo_1	Mycoplasma was eliminated with MC-210.
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_4849
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		HamF10 + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Isozyme		LD, NP
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	0
		User's Publication	6

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Reference

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User's Publication

17214  Sheng T, Ho SWT, Ooi WF, Xu C, Xing M, Padmanabhan N, Huang KK, Ma L, Ray M, Guo YA, Sim NL, Anene-Nzelu CG, Chang MM, Razavi-Mohseni M, Beer MA, Foo RSY, Sundar R, Chan YH, Tan ALK, Ong X, Skanderup AJ, White KP, Jha S, Tan P.  Integrative epigenomic and high-throughput functional enhancer profiling reveals determinants of enhancer heterogeneity in gastric cancer  Genome Med  2021  13(1):158  PubMed ID: 34635154   DOI: 10.1186/s13073-021-00970-3

14804  Xing M, Ooi WF, Tan J, Qamra A, Lee PH, Li Z, Xu C, Padmanabhan N, Lim JQ, Guo YA, Yao X, Amit M, Ng LM, Sheng T, Wang J, Huang KK, Anene-Nzelu CG, Ho SWT, Ray M, Ma L, Fazzi G, Lim KJ, Wijaya GC, Zhang S, Nandi T, Yan T, Chang MM, Das K, Isa ZFA, Wu J, Poon PSY, Lam YN, Lin JS, Tay ST, Lee MH, Tan ALK, Ong X, White K, Rozen SG, Beer M, Foo RSY, Grabsch HI, Skanderup AJ, Li S, Teh BT, Tan P.  Genomic and epigenomic EBF1 alterations modulate TERT expression in gastric cancer  J Clin Invest  2020  130(6):3005-3020  PubMed ID: 32364535   DOI: 10.1172/JCI126726

11280  Ueta K, Otowa Y, Kakeji Y, Hirashima M.  PROX1 Is Associated with Cancer Progression and Prognosis in Gastric Cancer.  Anticancer Res.  2018    PubMed ID: 30396930   DOI: 10.21873/anticanres.12966

10661  Keller S, Kneissl J, Grabher-Meier V, Heindl S, Hasenauer J, Maier D, Mattes J, Winter P, Luber B.  Evaluation of epidermal growth factor receptor signaling effects in gastric cancer cell lines by detailed motility-focused phenotypic characterization linked with molecular analysis.  BMC Cancer  2017  17:845  PubMed ID: 29237412   DOI: 10.1186/s12885-017-3822-3

6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x

18311  Mitsuoka K, Miyoshi S, Kato Y, Murakami Y, Utsumi R, Kubo Y, Noda A, Nakamura Y, Nishimura S, Tsuji A.  Cancer detection using a PET tracer, 11C-glycylsarcosine, targeted to H+/peptide transporter  J Nucl Med  2008  49(4):615-22  PubMed ID: 18344442   DOI: 10.2967/jnumed.107.048231