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Cell No. : Cell Name
RCB1036 : CJM Cl5  update : 2022/03/28
CommentHuman cell line derived from metastasis of cancer occurred in oral cavity, the same patient as T3M-1 Clone2 and T3M-1 Cl-10.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Okabe, Tetsuro
Originator Okabe, Tetsuro
Year of deposit 1994
Another name CJM-15 cl5-3
Original cell CJM
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Tissue oral, the thoracic cavity meta
Metastastatic ability Yes
Metastatsd tissue the thoracic cavity
Classification cancer
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8665
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF10 + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 58-63(50) : 58(3),59(4),60(31),61(3),62(6),63(3)
STR(human) OK
Reference information Reference 5
User's Publication 0


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Reference
1998  Sato K, Fujii Y, Kakiuchi T, Kasono K, Imamura H, Kondo Y, Mano H, Okabe T, Asano S, Takaku F, et al.  Paraneoplastic syndrome of hypercalcemia and leukocytosis caused by squamous carcinoma cells (T3M-1) producing parathyroid hormone-related protein, interleukin 1 alpha, and granulocyte colony-stimulating factor.  Cancer Res  1989  49:4740-6  PubMed ID: 2474371  
2018  Sato K, Mimura H, Han, D C, Kakiuchi T, Ueyama Y, Ohkawa H, Okabe T, Kondo Y, Ohsawa N, Tsushima T, et al.  Production of bone-resorbing activity and colony-stimulating activity in vivo and in vitro by a human squamous cell carcinoma associated with hypercalcemia and leukocytosis.  J Clin Invest  1986  78:145-54  PubMed ID: 3487554  
2019  Okabe T, Takaku F  Long-term cultivation of a human colony-stimulating factor-producing cell line in a protein-free chemically defined medium.  Cancer Res  1984  44:4503-6  PubMed ID: 6331879  
2051  Okabe T, Suzuki A, Ohsawa N, Kosaka K, Terasima T.  Immune elimination of host fibroblasts for the cultivation of human tumors transplanted into nude mice.  Cancer Res  1979  39(10):4189-94  PubMed ID: 476654  
2017  Okabe T, Sato N, Kondo Y, Asano S, Ohsawa N, Kosaka K, Ueyama Y  Establishment and characterization of a human cancer cell line that produces human colony-stimulating factor.  Cancer Res  1978  38:3910-7  PubMed ID: 308840  

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