Cell No. : Cell Name
RCB1036 : CJM Cl5
update : 2022/12/20
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| Comment | Human cell line derived from metastasis of cancer occurred in oral cavity, the same patient as T3M-1 Clone2 and T3M-1 Cl-10. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Okabe, Tetsuro
|
| Originator |
Okabe, Tetsuro
|
| Year of deposit |
1994
|
| Another name |
CJM-15 cl5-3
|
| Original cell |
CJM
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Tissue |
oral, the thoracic cavity meta
|
| Metastatic ability |
Yes
|
| Metastatic tissue |
the thoracic cavity
|
| Classification |
cancer
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_8665
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
|
Adherent cells
|
| Culture medium |
|
HamF10 + 10% CS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 2-4 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
58-63(50) : 58(3),59(4),60(31),61(3),62(6),63(3)
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
5
|
| User's Publication |
0
|
| Reference |
|
1998
Sato K, Fujii Y, Kakiuchi T, Kasono K, Imamura H, Kondo Y, Mano H, Okabe T, Asano S, Takaku F, et al.
Paraneoplastic syndrome of hypercalcemia and leukocytosis caused by squamous carcinoma cells (T3M-1) producing parathyroid hormone-related protein, interleukin 1 alpha, and granulocyte colony-stimulating factor.
Cancer Res
1989
49:4740-6
PubMed ID: 2474371
|
|
2018
Sato K, Mimura H, Han, D C, Kakiuchi T, Ueyama Y, Ohkawa H, Okabe T, Kondo Y, Ohsawa N, Tsushima T, et al.
Production of bone-resorbing activity and colony-stimulating activity in vivo and in vitro by a human squamous cell carcinoma associated with hypercalcemia and leukocytosis.
J Clin Invest
1986
78:145-54
PubMed ID: 3487554
DOI: 10.1172/JCI112544
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2019
Okabe T, Takaku F
Long-term cultivation of a human colony-stimulating factor-producing cell line in a protein-free chemically defined medium.
Cancer Res
1984
44:4503-6
PubMed ID: 6331879
|
|
2051
Okabe T, Suzuki A, Ohsawa N, Kosaka K, Terasima T.
Immune elimination of host fibroblasts for the cultivation of human tumors transplanted into nude mice.
Cancer Res
1979
39(10):4189-94
PubMed ID: 476654
|
|
2017
Okabe T, Sato N, Kondo Y, Asano S, Ohsawa N, Kosaka K, Ueyama Y
Establishment and characterization of a human cancer cell line that produces human colony-stimulating factor.
Cancer Res
1978
38:3910-7
PubMed ID: 308840
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