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Cell No. : Cell Name
RCB1033 : NOS-2  update : 2022/09/16
CommentHuman osteosarcoma producing osteoid in vitro and in vivo(mouse).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Motoyama, Teiichi
Originator Hotta, Tetsuo & Motoyama, Teiichi
Year of deposit 1994
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 11 years
Tissue bone
Disease name osteosarcoma
Metastatic ability No
Classification cancer
Year of origin 1989
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_3351
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : once/1-2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 46-116(50) : /56(3),57(5),58(4),59(4),60(5),61(3)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 3

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1935  Hotta T, Motoyama T, Watanabe H  Three human osteosarcoma cell lines exhibiting different phenotypic expressions.  Acta Pathol Jpn  1992  42:595-603  PubMed ID: 1449054   DOI: 10.1111/j.1440-1827.1992.tb03110.x

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User's Publication
17357  Hayashi T, Yamamoto N, Kurosawa G, Tajima K, Kondo M, Hiramatsu N, Kato Y, Tanaka M, Yamaguchi H, Kurosawa Y, Yamada H, Fujita N.  A Novel High-Throughput Screening Method for a Human Multicentric Osteosarcoma-Specific Antibody and Biomarker Using a Phage Display-Derived Monoclonal Antibody  Cancers (Basel)  2022  14(23):5829  PubMed ID: 36497311   DOI: 10.3390/cancers14235829
6431  Shibuya H, Hamamura K, Hotta H, Matsumoto Y, Nishida Y, Hattori H, Furukawa K, Ueda M, Furukawa K.  Enhancement of malignant properties of human osteosarcoma cells with disialyl gangliosides GD2/GD3.  Cancer Sci  2012  103(9):1656-64  PubMed ID: 22632091   DOI: 10.1111/j.1349-7006.2012.02344.x
2671  Nagano, Satoshi, Yuge, Kentaro, Fukunaga, Mari, Terazaki, Yasuhiro, Fujiwara, Hisayoshi, Komiya, Setsuro, Kosai, Ken-Ichiro  Gene therapy eradicating distant disseminated micro-metastases by optimal cytokine expression in the primary lesion only: novel concepts for successful cytokine gene therapy.  Int J Oncol  2004  24:549-58  PubMed ID: 14767539  

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