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Cell No. : Cell Name
RCB1027 : MEB4  update : 2022/03/28
CommentNot expressing melanoma antigen. Control for GM-95(RCB1026) cell line.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
RIKEN Center for Brain Science

Hirosawa 2-1, Wako, Saitama 351-0198, Japan
Dr.Hirabayashi, Yoshio
Fax. +81-48-467-6372
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hirabayashi, Yoshio
Originator Sakiyama, Hisako
Year of deposit 1994
Original cell B16 melanoma
Animal _mouse < Mammals
Strain name C57BL/6J
Tissue skin
Genetics melanoma
Classification cancer
History Yakiyama, Hisako - Hirabayashi, Yoshio
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4772
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 6
User's Publication 7

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16186  Mikhail Matrosovich, Takashi Suzuki, Yoshio Hirabayashi, Wolfgang Garten, Robert G Webster, Hans-Dieter Klenk  Gangliosides are not essential for influenza virus infection  Glycoconj J  2006  23(1-2):107-13  PubMed ID: 16575528   DOI: 10.1007/s10719-006-5443-y
16192  Reiko Ishitsuka, Akiko Yamaji-Hasegawa, Asami Makino, Yoshio Hirabayashi, Toshihide Kobayashi  A lipid-specific toxin reveals heterogeneity of sphingomyelin-containing membranes  Biophys J  2004  86(1 Pt 1):296-307  PubMed ID: 14695271   DOI: 10.1016/S0006-3495(04)74105-3
16191  H Sprong, S Degroote, T Claessens, J van Drunen, V Oorschot, B H Westerink, Y Hirabayashi, J Klumperman, P van der Sluijs, G van Meer  Glycosphingolipids are required for sorting melanosomal proteins in the Golgi complex  J Cell Biol  2001  155(3):369-80  PubMed ID: 11673476   DOI: 10.1083/jcb.200106104
16185  Hidari KIPJ, S Ichikawa, T Fujita, H Sakiyama, Y Hirabayashi  Complete removal of sphingolipids from the plasma membrane disrupts cell to substratum adhesion of mouse melanoma cells  J Biol Chem  1996  271(24):14636-41  PubMed ID: 8662871   DOI: 10.1074/jbc.271.24.14636
1893  S Ichikawa, N Nakajo, H Sakiyama, Y Hirabayashi   A mouse B16 melanoma mutant deficient in glycolipids  Proc Natl Acad Sci U S A  1994  91(7):2703-7  PubMed ID: 8146177   DOI: 10.1073/pnas.91.7.2703
1892  Nozue M, Sakiyama H, Tsuchiya K, Hirabayashi Y, Taniguchi M  Melanoma antigen expression and metastatic ability of mutant B16 melanoma clones.  Int J Cancer  1988  42:734-8  PubMed ID: 3053466   DOI: 10.1002/ijc.2910420518

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User's Publication
9665  Szerencsei RT, Ginger RS, Green MR, Schnetkamp PP.  Identification and Characterization of K(+)-Dependent Na(+)-Ca(2+) Exchange Transport in Pigmented MEB4 Cells Mediated by NCKX4.  Biochemistry  2016  55:2704-12  PubMed ID: 27093457   DOI: 10.1021/acs.biochem.6b00017
9103  Lorena Sánchez-Felipe, Enrique Villar, Isabel Muñoz-Barroso  Entry of Newcastle Disease Virus Into the Host Cell: Role of Acidic pH and Endocytosis  Biochim Biophys Acta  2014  1838 (1 Pt B), 300-9  PubMed ID: 23994097   DOI: 10.1016/j.bbamem.2013.08.008
16031  Sánchez-Felipe L, Villar E, Muñoz-Barroso I.  α2-3- and α2-6- N-linked sialic acids allow efficient interaction of Newcastle Disease Virus with target cells  Glycoconj J   2012  29(7):539-49  PubMed ID: 22869099   DOI: 10.1007/s10719-012-9431-0
7729  van der Poel S, Wolthoorn J, van den Heuvel D, Egmond M, Groux-Degroote S, Neumann S, Gerritsen H, van Meer G, Sprong H.  Hyperacidification of trans-Golgi network and endo/lysosomes in melanocytes by glucosylceramide-dependent V-ATPase activity.  Traffic  2011  12:1634-47  PubMed ID: 21810155   DOI: 10.1111/j.1600-0854.2011.01263.x
2655  Matsushima-Hibiya, Yuko, Watanabe, Masahiko, Hidari, Kazuya I-P, Miyamoto, Daisei, Suzuki, Yasuo, Kasama, Takeshi, Kasama, Takashi, Koyama, Kotaro, Sugimura, Takashi, Wakabayashi, Keiji  Identification of glycosphingolipid receptors for pierisin-1, a guanine-specific ADP-ribosylating toxin from the cabbage butterfly.  J Biol Chem  2003  278:9972-8  PubMed ID: 12645583   DOI: 10.1074/jbc.m212114200
2558  Kanazawa T, Watanabe M, Matsushima-Hibiya Y, Kono T, Tanaka N, Koyama K, Sugimura T, Wakabayashi K  Distinct roles for the N- and C-terminal regions in the cytotoxicity of pierisin-1, a putative ADP-ribosylating toxin from cabbage butterfly, against mammalian cells.  Proc Natl Acad Sci U S A  2001  98:2226-31  PubMed ID: 11226221   DOI: 10.1073/pnas.051628898
9031  S Ablan, S S Rawat, R Blumenthal, A Puri  Entry of Influenza Virus Into a Glycosphingolipid-Deficient Mouse Skin Fibroblast Cell Line  Arch Virol  2001  146 (11), 2227-38  PubMed ID: 11765924   DOI: 10.1007/s007050170033

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