細胞番号 : 細胞名 RCB1026 : GM95  update : 2022/03/28
細胞特性(Comment:英)			Glucosylceramide synthetase-deficient B16 melanoma mutant.
細胞特性(日)			グルコシルセラミド合成酵素欠損株。糖脂質を持たない。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)			A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
使用条件(日)			営利目的か非営利目的かを問わず、いかなる使用につきましても、承諾者(寄託者)から承諾を得ることが必要です。
備考(英)
備考(日)
承諾者住所			× Japanese 住所 351-0198 埼玉県和光市広沢2-1 理化学研究所   神経代謝脳神経科学研究センター 機構研究チーム 平林研究ユニット 平林 義雄 先生 Fax. 048-467-6372 English Address RIKEN Center for Brain Science Hirosawa 2-1, Wako, Saitama 351-0198, Japan Dr. Hirabayashi, Yoshio Fax. +81-48-467-6372
提供申込書類(英)			Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日)			依頼書C-0001.pdf   承諾書C-0002.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf
			提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料			手数料とお支払いについてはこちらをご覧ください。
細胞基本情報		寄託者	Hirabayashi, Yoshio
		樹立者	Sakiyama, Hisako
		寄託日	1994
		元の細胞	MEB4 (RCB1027)
		動物種	_mouse < Mammals
		動物系統名	C57BL/6J
		採取組織	skin
		病名	B16 melanoma mutant
		遺伝的特徴	glucosylceramide synthetase deficient
		細胞分類	mutant
		メモ_1	Mycoplasma was eliminated with MC-210 by the depositor.
		細胞寿命	infinite
		細胞形態	fibroblast-like
		Cellosaurus(Expasy)	CVCL_F938
細胞培養・検査情報			寄託時情報	ロット情報
	培地・試薬情報		培地・試薬一覧はこちらをご覧ください。
		培養形態		Adherent cells
		培地		DMEM (low glucose) + 10% FBS
		抗生物質		Free
		継代方法		0.25% Trypsin
		継代密度		1 : 8 split
		継代・培地交換頻度		Subculture : 2 times/week
		培養最適温度		37 ℃
		二酸化炭素濃度		5 %
		凍結培地		Medium + 10% DMSO
		凍結方法		Slow freezing
		マイコプラズマ/アコレプラズマ		(-)
		マウス系統検査		OK
		アイソザイム検査		LD, NP, G6PD
画像情報			寄託時情報	ロット情報
文献情報		Reference(英)	11件
		Reference(日)	0件
		利用者成果(英)	19件
		利用者成果(日)	0件

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Reference(英)

16186  Mikhail Matrosovich, Takashi Suzuki, Yoshio Hirabayashi, Wolfgang Garten, Robert G Webster, Hans-Dieter Klenk  Gangliosides are not essential for influenza virus infection  Glycoconj J  2006  23(1-2):107-13  PubMed ID: 16575528   DOI: 10.1007/s10719-006-5443-y

16188  Ayako Kohyama-Koganeya, Takeshi Sasamura, Eriko Oshima, Emiko Suzuki, Shoko Nishihara, Ryu Ueda, Yoshio Hirabayashi  Drosophila glucosylceramide synthase: a negative regulator of cell death mediated by proapoptotic factors  J Biol Chem  2004  279(34):35995-6002  PubMed ID: 15210713   DOI: 10.1074/jbc.M400444200

16192  Reiko Ishitsuka, Akiko Yamaji-Hasegawa, Asami Makino, Yoshio Hirabayashi, Toshihide Kobayashi  A lipid-specific toxin reveals heterogeneity of sphingomyelin-containing membranes  Biophys J  2004  86(1 Pt 1):296-307  PubMed ID: 14695271   DOI: 10.1016/S0006-3495(04)74105-3

16191  H Sprong, S Degroote, T Claessens, J van Drunen, V Oorschot, B H Westerink, Y Hirabayashi, J Klumperman, P van der Sluijs, G van Meer  Glycosphingolipids are required for sorting melanosomal proteins in the Golgi complex  J Cell Biol  2001  155(3):369-80  PubMed ID: 11673476   DOI: 10.1083/jcb.200106104

16182  T Kobayashi, Y Hirabayashi  Lipid membrane domains in cell surface and vacuolar systems  Glycoconj J  2000  17(3 -4):163-71  PubMed ID: 11201787   DOI: 10.1023/a:1026528921085

16184  H Komori, S Ichikawa, Y Hirabayashi, M Ito  Regulation of UDP-glucose:ceramide glucosyltransferase-1 by ceramide  FEBS Lett  2000  475(3):247-50  PubMed ID: 10869565   DOI: 10.1016/s0014-5793(00)01696-3

16189  H Komori, S Ichikawa, Y Hirabayashi, M Ito  Regulation of intracellular ceramide content in B16 melanoma cells. Biological implications of ceramide glycosylation  J Biol Chem  1999  274(13):8981-7  PubMed ID: 10085144   DOI: 10.1074/jbc.274.13.8981

16187  S Ichikawa, Y Hirabayashi  Glucosylceramide synthase and glycosphingolipid synthesis  Trends Cell Biol  1998  8(5):198-202  PubMed ID: 9695839   DOI: 10.1016/s0962-8924(98)01249-5

16185  Hidari KIPJ, S Ichikawa, T Fujita, H Sakiyama, Y Hirabayashi  Complete removal of sphingolipids from the plasma membrane disrupts cell to substratum adhesion of mouse melanoma cells  J Biol Chem  1996  271(24):14636-41  PubMed ID: 8662871   DOI: 10.1074/jbc.271.24.14636

1893  S Ichikawa, N Nakajo, H Sakiyama, Y Hirabayashi   A mouse B16 melanoma mutant deficient in glycolipids  Proc Natl Acad Sci U S A  1994  91(7):2703-7  PubMed ID: 8146177   DOI: 10.1073/pnas.91.7.2703

1892  Nozue M, Sakiyama H, Tsuchiya K, Hirabayashi Y, Taniguchi M  Melanoma antigen expression and metastatic ability of mutant B16 melanoma clones.  Int J Cancer  1988  42:734-8  PubMed ID: 3053466   DOI: 10.1002/ijc.2910420518

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Reference(日)

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利用者成果(英)

21069  Sorin MN, Di Maio A, Silva LM, Ebert D, Delannoy CP, Nguyen NK, Guerardel Y, Chai W, Halary F, Renaudin-Autain K, Liu Y, Bressollette-Bodin C, Stehle T, McIlroy D.  Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus.  Cell Rep  2023  42(2):112114  PubMed ID: 36790933   DOI: 10.1016/j.celrep.2023.112114

20198  Arumugam S, Schmieder S, Pezeshkian W, Becken U, Wunder C, Chinnapen D, Ipsen JH, Kenworthy AK, Lencer W, Mayor S, Johannes L.  Ceramide structure dictates glycosphingolipid nanodomain assembly and function.  Nat Commun  2021  12(1):3675  PubMed ID: 34135326   DOI: 10.1038/s41467-021-23961-9

12684  Lai CH, Hütter J, Hsu CW, Tanaka H, Varela-Aramburu S, De Cola L, Lepenies B, Seeberger PH.  Analysis of Carbohydrate-Carbohydrate Interactions Using Sugar-Functionalized Silicon Nanoparticles for Cell Imaging  Nano Lett  2016  16(1):807-11  PubMed ID: 26652315   DOI: 10.1021/acs.nanolett.5b04984

9471  Nobumasa Takasugi, Tomoki Sasaki, Mitsuru Shinohara, Takeshi Iwatsubo, Taisuke Tomita   Synthetic Ceramide Analogues Increase Amyloid-β 42 Production by Modulating γ-Secretase Activity  Biochem Biophys Res Commun  2015  457(2):194-9  PubMed ID: 25545059   DOI: 10.1016/j.bbrc.2014.12.087

15241  Molina-Guijarro JM, García C, Macías Á, García-Fernández LF, Moreno C, Reyes F, Martínez-Leal JF, Fernández R, Martínez V, Valenzuela C, Lillo MP, Galmarini CM.  Elisidepsin Interacts Directly with Glycosylceramides in the Plasma Membrane of Tumor Cells to Induce Necrotic Cell Death  PLoS One  2015  10(10):e0140782  PubMed ID: 26474061   DOI: 10.1371/journal.pone.0140782

9103  Lorena Sánchez-Felipe, Enrique Villar, Isabel Muñoz-Barroso  Entry of Newcastle Disease Virus Into the Host Cell: Role of Acidic pH and Endocytosis  Biochim Biophys Acta  2014  1838 (1 Pt B), 300-9  PubMed ID: 23994097   DOI: 10.1016/j.bbamem.2013.08.008

16197  Pastrana DV, Ray U, Magaldi TG, Schowalter RM, Çuburu N, Buck CB.  BK polyomavirus genotypes represent distinct serotypes with distinct entry tropism  J Virol   2013  ;87(18):10105-13  PubMed ID: 23843634   DOI: 10.1128/JVI.01189-13

16031  Sánchez-Felipe L, Villar E, Muñoz-Barroso I.  α2-3- and α2-6- N-linked sialic acids allow efficient interaction of Newcastle Disease Virus with target cells  Glycoconj J   2012  29(7):539-49  PubMed ID: 22869099   DOI: 10.1007/s10719-012-9431-0

7729  van der Poel S, Wolthoorn J, van den Heuvel D, Egmond M, Groux-Degroote S, Neumann S, Gerritsen H, van Meer G, Sprong H.  Hyperacidification of trans-Golgi network and endo/lysosomes in melanocytes by glucosylceramide-dependent V-ATPase activity.  Traffic  2011  12:1634-47  PubMed ID: 21810155   DOI: 10.1111/j.1600-0854.2011.01263.x

7963  Tamboli IY, Hampel H, Tien NT, Tolksdorf K, Breiden B, Mathews PM, Saftig P, Sandhoff K, Walter J.  Sphingolipid storage affects autophagic metabolism of the amyloid precursor protein and promotes Abeta generation.  J. Neurosci.  2011  31:1837-49  PubMed ID: 21289194   DOI: 10.1523/JNEUROSCI.2954-10.2011

16290  Rawat SS, Zimmerman C, Johnson BT, Cho E, Lockett SJ, Blumenthal R, Puri A.  Restricted lateral mobility of plasma membrane CD4 impairs HIV-1 envelope glycoprotein mediated fusion  Mol Membr Biol  2008  25(1):83-94  PubMed ID: 18097956   DOI: 10.1080/09687680701613713

16190  Michio Shimamura, Yi-Ying Huang, Naoki Okamoto, Yutaka Watanabe, Yoshiko Murakami, Taroh Kinoshita, Yoshio Hirabayashi, Chikara Murakata, Yukishige Ito, Tomoya Ogawa  Glycolipids with nonreducing end alpha-mannosyl residues that have the potential to activate invariant Valpha19 NKT cells  FEBS J  2007  274(11):2921-32  PubMed ID: 17488284   DOI: 10.1111/j.1742-4658.2007.05826.x

3410  Tamboli, Irfan Y, Prager, Kai, Barth, Esther, Heneka, Michael, Sandhoff, Konrad, Walter, Jochen  Inhibition of glycosphingolipid biosynthesis reduces secretion of the beta-amyloid precursor protein and amyloid beta-peptide.  J Biol Chem  2005  280:28110-7  PubMed ID: 15923191   DOI: 10.1074/jbc.M414525200

16181  Rawat SS, Eaton J, Gallo SA, Martin TD, Ablan S, Ratnayake S, Viard M, KewalRamani VN, Wang JM, Blumenthal R, Puri A.  Functional expression of CD4, CXCR4, and CCR5 in glycosphingolipid-deficient mouse melanoma GM95 cells and susceptibility to HIV-1 envelope glycoprotein-triggered membrane fusion  Virology  2004  318(1):55-65  PubMed ID: 14972535   DOI: 10.1016/j.virol.2003.08.042

16288  Rawat SS, Gallo SA, Eaton J, Martin TD, Ablan S, KewalRamani VN, Wang JM, Blumenthal R, Puri A.  Elevated expression of GM3 in receptor-bearing targets confers resistance to human immunodeficiency virus type 1 fusion  J Virol   2004  78(14):7360-8  PubMed ID: 15220409   DOI: 10.1128/JVI.78.14.7360-7368.2004

16198  Veldman RJ, Mita A, Cuvillier O, Garcia V, Klappe K, Medin JA, Campbell JD, Carpentier S, Kok JW, Levade T.  The absence of functional glucosylceramide synthase does not sensitize melanoma cells for anticancer drugs  FASEB J  2003  17(9):1144-6  PubMed ID: 12692077   DOI: 10.1096/fj.02-1053fje

16199  Shah AH, Lipton HL.  Low-neurovirulence Theiler's viruses use sialic acid moieties on N-linked oligosaccharide structures for attachment  Virology .  2002  304(2):443-50  PubMed ID: 12504583   DOI: 10.1006/viro.2002.1735

9031  S Ablan, S S Rawat, R Blumenthal, A Puri  Entry of Influenza Virus Into a Glycosphingolipid-Deficient Mouse Skin Fibroblast Cell Line  Arch Virol  2001  146 (11), 2227-38  PubMed ID: 11765924   DOI: 10.1007/s007050170033

1402  Nakajima H, Miura Y, Yamagata T.  Glycosylation of amphipathic lactoside primers with consequent inhibition of endogenous glycosphingolipid synthesis  J Biochem  1998  124(1):148-56.  PubMed ID: 9644257   DOI: 10.1093/oxfordjournals.jbchem.a022073

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