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Cell No. : Cell Name
RCB1026 : GM95  update : 2022/03/28
CommentGlucosylceramide synthetase-deficient B16 melanoma mutant.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
RIKEN Center for Brain Science

Hirosawa 2-1, Wako, Saitama 351-0198, Japan
Dr.Hirabayashi, Yoshio
Fax. +81-48-467-6372
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hirabayashi, Yoshio
Originator Sakiyama, Hisako
Year of deposit 1994
Original cell MEB4 (RCB1027)
Animal mouse < Mammals
Strain name C57BL/6J
Tissue skin
Disease name B16 melanoma mutant
Genetics glucosylceramide synthetase deficient
Classification mutant
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_F938
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD
deposit info
lot info
Reference information Reference 12
User's Publication 17

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16190  Michio Shimamura, Yi-Ying Huang, Naoki Okamoto, Yutaka Watanabe, Yoshiko Murakami, Taroh Kinoshita, Yoshio Hirabayashi, Chikara Murakata, Yukishige Ito, Tomoya Ogawa  Glycolipids with nonreducing end alpha-mannosyl residues that have the potential to activate invariant Valpha19 NKT cells  FEBS J  2007  274(11):2921-32  PubMed ID: 17488284   DOI: 10.1111/j.1742-4658.2007.05826.x
16186  Mikhail Matrosovich, Takashi Suzuki, Yoshio Hirabayashi, Wolfgang Garten, Robert G Webster, Hans-Dieter Klenk  Gangliosides are not essential for influenza virus infection  Glycoconj J  2006  23(1-2):107-13  PubMed ID: 16575528   DOI: 10.1007/s10719-006-5443-y
16188  Ayako Kohyama-Koganeya, Takeshi Sasamura, Eriko Oshima, Emiko Suzuki, Shoko Nishihara, Ryu Ueda, Yoshio Hirabayashi  Drosophila glucosylceramide synthase: a negative regulator of cell death mediated by proapoptotic factors  J Biol Chem  2004  279(34):35995-6002  PubMed ID: 15210713   DOI: 10.1074/jbc.M400444200
16192  Reiko Ishitsuka, Akiko Yamaji-Hasegawa, Asami Makino, Yoshio Hirabayashi, Toshihide Kobayashi  A lipid-specific toxin reveals heterogeneity of sphingomyelin-containing membranes  Biophys J  2004  86(1 Pt 1):296-307  PubMed ID: 14695271   DOI: 10.1016/S0006-3495(04)74105-3
16191  H Sprong, S Degroote, T Claessens, J van Drunen, V Oorschot, B H Westerink, Y Hirabayashi, J Klumperman, P van der Sluijs, G van Meer  Glycosphingolipids are required for sorting melanosomal proteins in the Golgi complex  J Cell Biol  2001  155(3):369-80  PubMed ID: 11673476   DOI: 10.1083/jcb.200106104
16182  T Kobayashi, Y Hirabayashi  Lipid membrane domains in cell surface and vacuolar systems  Glycoconj J  2000  17(3 -4):163-71  PubMed ID: 11201787   DOI: 10.1023/a:1026528921085
16184  H Komori, S Ichikawa, Y Hirabayashi, M Ito  Regulation of UDP-glucose:ceramide glucosyltransferase-1 by ceramide  FEBS Lett  2000  475(3):247-50  PubMed ID: 10869565   DOI: 10.1016/s0014-5793(00)01696-3
16189  H Komori, S Ichikawa, Y Hirabayashi, M Ito  Regulation of intracellular ceramide content in B16 melanoma cells. Biological implications of ceramide glycosylation  J Biol Chem  1999  274(13):8981-7  PubMed ID: 10085144   DOI: 10.1074/jbc.274.13.8981
16187  S Ichikawa, Y Hirabayashi  Glucosylceramide synthase and glycosphingolipid synthesis  Trends Cell Biol  1998  8(5):198-202  PubMed ID: 9695839   DOI: 10.1016/s0962-8924(98)01249-5
16185  Hidari KIPJ, S Ichikawa, T Fujita, H Sakiyama, Y Hirabayashi  Complete removal of sphingolipids from the plasma membrane disrupts cell to substratum adhesion of mouse melanoma cells  J Biol Chem  1996  271(24):14636-41  PubMed ID: 8662871   DOI: 10.1074/jbc.271.24.14636
16183  S Ichikawa, N Nakajo, H Sakiyama, Y Hirabayashi  A mouse B16 melanoma mutant deficient in glycolipids  Proc Natl Acad Sci U S A  1994  91(7):2703-7  PubMed ID: 8146177   DOI: 10.1073/pnas.91.7.2703
1892  Nozue M, Sakiyama H, Tsuchiya K, Hirabayashi Y, Taniguchi M  Melanoma antigen expression and metastatic ability of mutant B16 melanoma clones.  Int J Cancer  1988  42:734-8  PubMed ID: 3053466   DOI: 10.1002/ijc.2910420518

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User's Publication
21069  Sorin MN, Di Maio A, Silva LM, Ebert D, Delannoy CP, Nguyen NK, Guerardel Y, Chai W, Halary F, Renaudin-Autain K, Liu Y, Bressollette-Bodin C, Stehle T, McIlroy D.  Structural and functional analysis of natural capsid variants suggests sialic acid-independent entry of BK polyomavirus.  Cell Rep  2023  42(2):112114  PubMed ID: 36790933   DOI: 10.1016/j.celrep.2023.112114
20198  Arumugam S, Schmieder S, Pezeshkian W, Becken U, Wunder C, Chinnapen D, Ipsen JH, Kenworthy AK, Lencer W, Mayor S, Johannes L.  Ceramide structure dictates glycosphingolipid nanodomain assembly and function.  Nat Commun  2021  12(1):3675  PubMed ID: 34135326   DOI: 10.1038/s41467-021-23961-9
12684  Lai CH, Hütter J, Hsu CW, Tanaka H, Varela-Aramburu S, De Cola L, Lepenies B, Seeberger PH.  Analysis of Carbohydrate-Carbohydrate Interactions Using Sugar-Functionalized Silicon Nanoparticles for Cell Imaging  Nano Lett  2016  16(1):807-11  PubMed ID: 26652315   DOI: 10.1021/acs.nanolett.5b04984
9471  Nobumasa Takasugi, Tomoki Sasaki, Mitsuru Shinohara, Takeshi Iwatsubo, Taisuke Tomita   Synthetic Ceramide Analogues Increase Amyloid-β 42 Production by Modulating γ-Secretase Activity  Biochem Biophys Res Commun  2015  457(2):194-9  PubMed ID: 25545059   DOI: 10.1016/j.bbrc.2014.12.087
15241  Molina-Guijarro JM, García C, Macías Á, García-Fernández LF, Moreno C, Reyes F, Martínez-Leal JF, Fernández R, Martínez V, Valenzuela C, Lillo MP, Galmarini CM.  Elisidepsin Interacts Directly with Glycosylceramides in the Plasma Membrane of Tumor Cells to Induce Necrotic Cell Death  PLoS One  2015  10(10):e0140782  PubMed ID: 26474061   DOI: 10.1371/journal.pone.0140782
9103  Lorena Sánchez-Felipe, Enrique Villar, Isabel Muñoz-Barroso  Entry of Newcastle Disease Virus Into the Host Cell: Role of Acidic pH and Endocytosis  Biochim Biophys Acta  2014  1838 (1 Pt B), 300-9  PubMed ID: 23994097   DOI: 10.1016/j.bbamem.2013.08.008
16197  Pastrana DV, Ray U, Magaldi TG, Schowalter RM, Çuburu N, Buck CB.  BK polyomavirus genotypes represent distinct serotypes with distinct entry tropism  J Virol   2013  ;87(18):10105-13  PubMed ID: 23843634   DOI: 10.1128/JVI.01189-13
16031  Sánchez-Felipe L, Villar E, Muñoz-Barroso I.  α2-3- and α2-6- N-linked sialic acids allow efficient interaction of Newcastle Disease Virus with target cells  Glycoconj J   2012  29(7):539-49  PubMed ID: 22869099   DOI: 10.1007/s10719-012-9431-0
7729  van der Poel S, Wolthoorn J, van den Heuvel D, Egmond M, Groux-Degroote S, Neumann S, Gerritsen H, van Meer G, Sprong H.  Hyperacidification of trans-Golgi network and endo/lysosomes in melanocytes by glucosylceramide-dependent V-ATPase activity.  Traffic  2011  12:1634-47  PubMed ID: 21810155   DOI: 10.1111/j.1600-0854.2011.01263.x
7963  Tamboli IY, Hampel H, Tien NT, Tolksdorf K, Breiden B, Mathews PM, Saftig P, Sandhoff K, Walter J.  Sphingolipid storage affects autophagic metabolism of the amyloid precursor protein and promotes Abeta generation.  J. Neurosci.  2011  31:1837-49  PubMed ID: 21289194   DOI: 10.1523/JNEUROSCI.2954-10.2011
16290  Rawat SS, Zimmerman C, Johnson BT, Cho E, Lockett SJ, Blumenthal R, Puri A.  Restricted lateral mobility of plasma membrane CD4 impairs HIV-1 envelope glycoprotein mediated fusion  Mol Membr Biol  2008  25(1):83-94  PubMed ID: 18097956   DOI: 10.1080/09687680701613713
3410  Tamboli, Irfan Y, Prager, Kai, Barth, Esther, Heneka, Michael, Sandhoff, Konrad, Walter, Jochen  Inhibition of glycosphingolipid biosynthesis reduces secretion of the beta-amyloid precursor protein and amyloid beta-peptide.  J Biol Chem  2005  280:28110-7  PubMed ID: 15923191   DOI: 10.1074/jbc.M414525200
16181  Rawat SS, Eaton J, Gallo SA, Martin TD, Ablan S, Ratnayake S, Viard M, KewalRamani VN, Wang JM, Blumenthal R, Puri A.  Functional expression of CD4, CXCR4, and CCR5 in glycosphingolipid-deficient mouse melanoma GM95 cells and susceptibility to HIV-1 envelope glycoprotein-triggered membrane fusion  Virology  2004  318(1):55-65  PubMed ID: 14972535   DOI: 10.1016/j.virol.2003.08.042
16288  Rawat SS, Gallo SA, Eaton J, Martin TD, Ablan S, KewalRamani VN, Wang JM, Blumenthal R, Puri A.  Elevated expression of GM3 in receptor-bearing targets confers resistance to human immunodeficiency virus type 1 fusion  J Virol   2004  78(14):7360-8  PubMed ID: 15220409   DOI: 10.1128/JVI.78.14.7360-7368.2004
16198  Veldman RJ, Mita A, Cuvillier O, Garcia V, Klappe K, Medin JA, Campbell JD, Carpentier S, Kok JW, Levade T.  The absence of functional glucosylceramide synthase does not sensitize melanoma cells for anticancer drugs  FASEB J  2003  17(9):1144-6  PubMed ID: 12692077   DOI: 10.1096/fj.02-1053fje
16199  Shah AH, Lipton HL.  Low-neurovirulence Theiler's viruses use sialic acid moieties on N-linked oligosaccharide structures for attachment  Virology .  2002  304(2):443-50  PubMed ID: 12504583   DOI: 10.1006/viro.2002.1735
9031  S Ablan, S S Rawat, R Blumenthal, A Puri  Entry of Influenza Virus Into a Glycosphingolipid-Deficient Mouse Skin Fibroblast Cell Line  Arch Virol  2001  146 (11), 2227-38  PubMed ID: 11765924   DOI: 10.1007/s007050170033

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