Back
|
Cell No. : Cell Name
RCB1025 : RTH33 update : 2023/05/02 |
||||
| Comment | SV40-Adeno Vector transformed hepatocytes. Albumin secretion and urea synthesis were reported. | |||
| Comment from the depositor | ||||
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Tabata, Naoto | ||
| Originator | Tabata, Naoto | |||
| Year of deposit | 1994 | |||
| Animal | _rat < Mammals | |||
| Strain name | Wistar | |||
| Genus | Rattus | |||
| Species | norvegicus | |||
| Gender | Male | |||
| Tissue | liver | |||
| Classification | transformed | |||
| Recombinant | recombinant | |||
| Exogene | SV40 large T antigen gene | |||
| Memo_1 | Mycoplasma was eliminated with MC-210 by the depositor. | Lifespan | infinite | |
| Morphology | epithelial-like | |||
| Cellosaurus(Expasy) | CVCL_8753 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | DMEM (low glucose) + 10% FBS | |||
| Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 8 split |
| SC frequency | Subculture : 1-2 times/week, Medium Renewal : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma | (-) | |||
| Animal PCR | OK | |||
| Isozyme | LD, NP | |||
| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
Top
| Reference |
| 2287 Tabata, N., Katsuki, T., Kumura, K., Nakagawa, N., Kondo, K., Nagatomo, H., and Ninamishima, Y. Establishment and characterization of a rat hepatocyte line transformed by the SV40 T antigen gene Artif Organs Today 1993 3:43-56 |
Top
Back
CELL BANK Website