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Cell No. : Cell Name
RCB1025 : RTH33  update : 2022/03/28
CommentSV40-Adeno Vector transformed hepatocytes. Albumin secretion and urea synthesis were reported.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Tabata, Naoto
Originator Tabata, Naoto
Year of deposit 1994
Animal rat < Mammals
Strain name Wistar
Genus Rattus
Species norvegicus
Gender Male
Tissue liver
Classification transformed
Recombinant recombinant
Exogene SV40 large T antigen gene
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8753
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 1


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Reference
2287  Tabata, N., Katsuki, T., Kumura, K., Nakagawa, N., Kondo, K., Nagatomo, H., and Ninamishima, Y.  Establishment and characterization of a rat hepatocyte line transformed by the SV40 T antigen gene  Artif Organs Today  1993  3:43-56 

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User's Publication
12855  Sumii T, Nakano Y, Abe T, Nakashima K, Sera T, Kudo S.  The Effect of Nitric Oxide on Ammonia Decomposition in Co-cultures of Hepatocytes and Hepatic Stellate Cells  In Vitro Cell Dev Biol Anim  2016  52(6):625-31  PubMed ID: 26896067



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