Cell No. : Cell Name
RCB1024 : IMR-90-SV
update : 2024/06/12
|
| Comment | SV40 transformed human lung fibroblast. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Kaji, Kazuhiko
|
| Year of deposit |
1994
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
embryo
|
| Tissue |
lung
|
| Classification |
transformed
|
| History |
IMR, K. Kaji
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_6269
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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MEM + 10% FBS
|
| Antibiotics |
|
Free
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|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Isozyme |
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LD, NP
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| Chromosome mode |
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77-85(50) : /79(3),80(9),81(9),82(8),83(5),84(9)/
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| STR(human) |
|
OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
9
|
| User's Publication |
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19688
Sato K, Yang M, Nakamura K, Tanaka H, Hori M, Nishio M, Suzuki A, Hibi H, Toyokuni S.
Ferroptosis induced by plasma-activated Ringer's lactate solution prevents oral cancer progression
Oral Dis
2024
30(6):3912-3924
PubMed ID: 38047766
DOI: 10.1111/odi.14827
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17633
Lyu Q, Jiang L, Zheng H, Hayashi S, Sato K, Toyokuni S.
Diluted aqueous extract of heat-not-burn tobacco product smoke causes less oxidative damage in fibroblasts than conventional cigarette
J Clin Biochem Nutr
2022
71(1):55-63
PubMed ID: 35903603
DOI: 10.3164/jcbn.21-134
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12586
Sato K, Shi L, Ito F, Ohara Y, Motooka Y, Tanaka H, Mizuno M, Hori M, Hirayama T, Hibi H, Toyokuni S.
Non-thermal plasma specifically kills oral squamous cell carcinoma cells in a catalytic Fe(II)-dependent manner
J Clin Biochem Nutr
2019
65(1):8-15
PubMed ID: 31379408
DOI: 10.3164/jcbn.18-91
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10493
Hirako S, Tsuda H, Ito F, Okazaki Y, Hirayama T, Nagasawa H, Nakano T, Imai K, Kotani T, Kikkawa F, Toyokuni S.
Role of catalytic iron and oxidative stress in nitrofen-induced congenital diaphragmatic hernia and its amelioration by Saireito (TJ-114).
J Clin Biochem Nutr
2017
61:176-182
PubMed ID: 29203958
DOI: 10.3164/jcbn.17-17
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13749
Shi L, Ito F, Wang Y, Okazaki Y, Tanaka H, Mizuno M, Hori M, Hirayama T, Nagasawa H, Richardson DR, Toyokuni S.
Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy
Free Radic Biol Med
2017
108:904-917
PubMed ID: 28465262
DOI: 10.1016/j.freeradbiomed.2017.04.368
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9714
Ito F, Nishiyama T, Shi L, Mori M, Hirayama T, Nagasawa H, Yasui H, Toyokuni S.
Contrasting intra- and extracellular distribution of catalytic ferrous iron in ovalbumin-induced peritonitis.
Biochem. Biophys. Res. Commun.
2016
476:600-606
PubMed ID: 27262439
DOI: 10.1016/j.bbrc.2016.06.003
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12215
Lei Shi, Yue Wang, Fumiya Ito, Yasumasa Okazaki, Hiromasa Tanaka, Masaaki Mizuno, Masaru Hori, Des R Richardson, Shinya Toyokuni
Biphasic effects of l-ascorbate on the tumoricidal activity of non-thermal plasma against malignant mesothelioma cells
Arch Biochem Biophys
2016
605:109-16
PubMed ID: 27235332
DOI: 10.1016/j.abb.2016.05.016
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19027
Ishii G, Sangai T, Ito T, Hasebe T, Endoh Y, Sasaki H, Harigaya K, Ochiai A.
In vivo and in vitro characterization of human fibroblasts recruited selectively into human cancer stroma
Int J Cancer
2005
117(2):212-20
PubMed ID: 15900602
DOI: 10.1002/ijc.21199
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3039
Suzuki K, Kazui T, Yoshida M, Uno T, Kobayashi T, Kimura T, Yoshida T, Sugimura H
Drug-induced apoptosis and p53, BCL-2 and BAX expression in breast cancer tissues in vivo and in fibroblast cells in vitro.
Jpn J Clin Oncol
1999
29:323-31
PubMed ID: 10470656
DOI: 10.1093/jjco/29.7.323
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