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Cell No. : Cell Name
RCB1024 : IMR-90-SV  update : 2024/06/12
CommentSV40 transformed human lung fibroblast.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kaji, Kazuhiko
Year of deposit 1994
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling embryo
Tissue lung
Classification transformed
History IMR, K. Kaji
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_6269
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
Chromosome mode 77-85(50) : /79(3),80(9),81(9),82(8),83(5),84(9)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 9


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Reference
192  Nichols WW, Murphy DG, Cristofalo VJ, Toji LH, Greene AE, Dwight SA.  Characterization of a new human diploid cell strain, IMR-90.  Science  1977  196(4285):60-3  PubMed ID: 841339   DOI: 10.1126/science.841339

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User's Publication
19688  Sato K, Yang M, Nakamura K, Tanaka H, Hori M, Nishio M, Suzuki A, Hibi H, Toyokuni S.  Ferroptosis induced by plasma-activated Ringer's lactate solution prevents oral cancer progression  Oral Dis  2023  Dec 4  PubMed ID: 38047766   DOI: 10.1111/odi.14827
17633  Lyu Q, Jiang L, Zheng H, Hayashi S, Sato K, Toyokuni S.  Diluted aqueous extract of heat-not-burn tobacco product smoke causes less oxidative damage in fibroblasts than conventional cigarette  J Clin Biochem Nutr  2022  71(1):55-63  PubMed ID: 35903603   DOI: 10.3164/jcbn.21-134
12586  Sato K, Shi L, Ito F, Ohara Y, Motooka Y, Tanaka H, Mizuno M, Hori M, Hirayama T, Hibi H, Toyokuni S.  Non-thermal plasma specifically kills oral squamous cell carcinoma cells in a catalytic Fe(II)-dependent manner  J Clin Biochem Nutr  2019  65(1):8-15  PubMed ID: 31379408   DOI: 10.3164/jcbn.18-91
10493  Hirako S, Tsuda H, Ito F, Okazaki Y, Hirayama T, Nagasawa H, Nakano T, Imai K, Kotani T, Kikkawa F, Toyokuni S.  Role of catalytic iron and oxidative stress in nitrofen-induced congenital diaphragmatic hernia and its amelioration by Saireito (TJ-114).  J Clin Biochem Nutr  2017  61:176-182  PubMed ID: 29203958   DOI: 10.3164/jcbn.17-17
13749  Shi L, Ito F, Wang Y, Okazaki Y, Tanaka H, Mizuno M, Hori M, Hirayama T, Nagasawa H, Richardson DR, Toyokuni S.  Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy  Free Radic Biol Med  2017  108:904-917  PubMed ID: 28465262   DOI: 10.1016/j.freeradbiomed.2017.04.368
9714  Ito F, Nishiyama T, Shi L, Mori M, Hirayama T, Nagasawa H, Yasui H, Toyokuni S.  Contrasting intra- and extracellular distribution of catalytic ferrous iron in ovalbumin-induced peritonitis.  Biochem. Biophys. Res. Commun.  2016  476:600-606  PubMed ID: 27262439   DOI: 10.1016/j.bbrc.2016.06.003
12215  Lei Shi, Yue Wang, Fumiya Ito, Yasumasa Okazaki, Hiromasa Tanaka, Masaaki Mizuno, Masaru Hori, Des R Richardson, Shinya Toyokuni   Biphasic effects of l-ascorbate on the tumoricidal activity of non-thermal plasma against malignant mesothelioma cells  Arch Biochem Biophys  2016  605:109-16  PubMed ID: 27235332   DOI: 10.1016/j.abb.2016.05.016
19027  Ishii G, Sangai T, Ito T, Hasebe T, Endoh Y, Sasaki H, Harigaya K, Ochiai A.  In vivo and in vitro characterization of human fibroblasts recruited selectively into human cancer stroma  Int J Cancer  2005  117(2):212-20  PubMed ID: 15900602   DOI: 10.1002/ijc.21199
3039  Suzuki K, Kazui T, Yoshida M, Uno T, Kobayashi T, Kimura T, Yoshida T, Sugimura H  Drug-induced apoptosis and p53, BCL-2 and BAX expression in breast cancer tissues in vivo and in fibroblast cells in vitro.  Jpn J Clin Oncol  1999  29:323-31  PubMed ID: 10470656   DOI: 10.1093/jjco/29.7.323



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