Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB1024 : IMR-90-SV  update : 2020/09/24
CommentSV40 transformed human lung fibroblast.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Kaji, Kazuhiko
Year of deposit 1994
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling embryo
Tissue lung
Classification transformed
History IMR, K. Kaji
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Chromosome mode 77-85(50) : /79(3),80(9),81(9),82(8),83(5),84(9)/
Images
deposit info
lot info
Reference information Reference 1
User's Publication 4


To topTop
Reference
192  Nichols WW, Murphy DG, Cristofalo VJ, Toji LH, Greene AE, Dwight SA.  Characterization of a new human diploid cell strain, IMR-90.  Science  1977  196(4285):60-3  PubMed ID: 841339

To topTop
User's Publication
10493  Hirako S, Tsuda H, Ito F, Okazaki Y, Hirayama T, Nagasawa H, Nakano T, Imai K, Kotani T, Kikkawa F, Toyokuni S.  Role of catalytic iron and oxidative stress in nitrofen-induced congenital diaphragmatic hernia and its amelioration by Saireito (TJ-114).  J Clin Biochem Nutr  2017  61:176-182  PubMed ID: 29203958
9714  Ito F, Nishiyama T, Shi L, Mori M, Hirayama T, Nagasawa H, Yasui H, Toyokuni S.  Contrasting intra- and extracellular distribution of catalytic ferrous iron in ovalbumin-induced peritonitis.  Biochem. Biophys. Res. Commun.  2016  476:600-606  PubMed ID: 27262439
12215  Lei Shi, Yue Wang, Fumiya Ito, Yasumasa Okazaki, Hiromasa Tanaka, Masaaki Mizuno, Masaru Hori, Des R Richardson, Shinya Toyokuni   Biphasic effects of l-ascorbate on the tumoricidal activity of non-thermal plasma against malignant mesothelioma cells  Arch Biochem Biophys  2016  605:109-16  PubMed ID: 27235332
3039  Suzuki K, Kazui T, Yoshida M, Uno T, Kobayashi T, Kimura T, Yoshida T, Sugimura H  Drug-induced apoptosis and p53, BCL-2 and BAX expression in breast cancer tissues in vivo and in fibroblast cells in vitro.  Jpn J Clin Oncol  1999  29:323-31  PubMed ID: 10470656



Back Back Return Top Page