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Cell No. : Cell Name
RCB1022 : T3M-11  update : 2022/03/28
CommentHuman cell line derived from lung cancer (small cell carcinoma). Expressing insulin-like growth factor II (IGF-II).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Okabe, Tetsuro
Originator Okabe, Tetsuro
Year of deposit 1994
Another name ACTH
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Tissue lung
Disease name small cell carcinoma
Metastastatic ability Yes
Classification cancer
Year of origin 1982
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8066
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF10 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 3 split
SC frequency Subculture : 1-2 times/1-2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 44-47(50) : 44(2),45(8),46(38),47(2)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 2


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Reference
2024  Hagiwara K, Kobayashi T, Tobita M, Kikyo N, Yazaki Y, Okabe T  Isolotion of a cDNA for a Growth Factor of Vascular Endothelial Cells from Human Lung Cancer Cells : Its Identity with Insulin-Like Growth Factor II  Jpn J Cancer Res  1995  86:202-207  PubMed ID: 7730145   DOI: 10.1111/j.1349-7006.1995.tb03040.x

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User's Publication
5833  Sato M, Muguruma N, Nakagawa T, Okamoto K, Kimura T, Kitamura S, Yano H, Sannomiya K, Goji T, Miyamoto H, Okahisa T, Mikasa H, Wada S, Iwata M, Takayama T.  High antitumor activity of pladienolide B and its derivative in gastric cancer.  Cancer Sci  2014  105(1):110-6  PubMed ID: 24635824   DOI: 10.1111/cas.12317
5870  Takahashi N, Fukushima T, Yorita K, Tanaka H, Chijiiwa K, Kataoka H.  Dickkopf-1 is overexpressed in human pancreatic ductal adenocarcinoma cells and is involved in invasive growth.  Int J Cancer.  2010  126(7):1611-20  PubMed ID: 19711349   DOI: 10.1002/ijc.24865



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