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Cell No. : Cell Name
RCB1022 : T3M-11 update : 2024/11/12 |
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| Comment | Human cell line derived from lung cancer (small cell carcinoma). Expressing insulin-like growth factor II (IGF-II). | |||
| Comment from the depositor | ||||
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Okabe, Tetsuro | ||
| Originator | Okabe, Tetsuro | |||
| Year of deposit | 1994 | |||
| Another name | ACTH | |||
| Animal | _human < Mammals | |||
| Genus | Homo | |||
| Species | sapiens | |||
| Race | Japanese | |||
| Tissue | lung | |||
| Disease name | small cell carcinoma | |||
| Metastatic ability | Yes | |||
| Classification | cancer | |||
| Year of origin | 1982 | |||
| Lifespan | infinite | |||
| Morphology | epithelial-like | |||
| Cellosaurus(Expasy) | CVCL_8066 | |||
| Medium | Medium List | |||
| Culture type | Adherent cells | |||
| Culture medium | HamF10 + 10% FBS | |||
| Antibiotics | Free | |||
| Passage method | 0.25% Trypsin | |||
| Culture information | Passage ratio | 1 : 3 split | ||
| SC frequency | Subculture : 1-2 times/1-2 weeks, Medium Renewal : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Isozyme | LD, NP | |||
| Chromosome mode | 44-47(50) : 44(2),45(8),46(38),47(2) | |||
| STR(human) | OK | |||
| Images | ||||
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| Reference information | Reference | 1 | ||
| User's Publication | 3 | |||
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| Reference |
| 2024 Hagiwara K, Kobayashi T, Tobita M, Kikyo N, Yazaki Y, Okabe T Isolotion of a cDNA for a Growth Factor of Vascular Endothelial Cells from Human Lung Cancer Cells : Its Identity with Insulin-Like Growth Factor II Jpn J Cancer Res 1995 86:202-207 PubMed ID: 7730145 DOI: 10.1111/j.1349-7006.1995.tb03040.x |
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| User's Publication |
| 22524 Kasai F, Fukushima M, Miyagi Y, Nakamura Y. Genetic diversity among the present Japanese population: evidence from genotyping of human cell lines established in Japan Hum Cell 2024 37(4):944-950 PubMed ID: 38639832 DOI: 10.1007/s13577-024-01055-0 |
| 5833 Sato M, Muguruma N, Nakagawa T, Okamoto K, Kimura T, Kitamura S, Yano H, Sannomiya K, Goji T, Miyamoto H, Okahisa T, Mikasa H, Wada S, Iwata M, Takayama T. High antitumor activity of pladienolide B and its derivative in gastric cancer. Cancer Sci 2014 105(1):110-6 PubMed ID: 24635824 DOI: 10.1111/cas.12317 |
| 5870 Takahashi N, Fukushima T, Yorita K, Tanaka H, Chijiiwa K, Kataoka H. Dickkopf-1 is overexpressed in human pancreatic ductal adenocarcinoma cells and is involved in invasive growth. Int J Cancer. 2010 126(7):1611-20 PubMed ID: 19711349 DOI: 10.1002/ijc.24865 |
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