Cell No. : Cell Name RCB1022 : T3M-11  update : 2024/11/12
Comment			Human cell line derived from lung cancer (small cell carcinoma). Expressing insulin-like growth factor II (IGF-II).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Okabe, Tetsuro
		Originator	Okabe, Tetsuro
		Year of deposit	1994
		Another name	ACTH
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Tissue	lung
		Disease name	small cell carcinoma
		Metastatic ability	Yes
		Classification	cancer
		Year of origin	1982
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_8066
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		HamF10 + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 3 split
		SC frequency		Subculture : 1-2 times/1-2 weeks, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Isozyme		LD, NP
		Chromosome mode		44-47(50) : 44(2),45(8),46(38),47(2)
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	2

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Reference

2024  Hagiwara K, Kobayashi T, Tobita M, Kikyo N, Yazaki Y, Okabe T  Isolotion of a cDNA for a Growth Factor of Vascular Endothelial Cells from Human Lung Cancer Cells : Its Identity with Insulin-Like Growth Factor II  Jpn J Cancer Res  1995  86:202-207  PubMed ID: 7730145   DOI: 10.1111/j.1349-7006.1995.tb03040.x

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User's Publication

5833  Sato M, Muguruma N, Nakagawa T, Okamoto K, Kimura T, Kitamura S, Yano H, Sannomiya K, Goji T, Miyamoto H, Okahisa T, Mikasa H, Wada S, Iwata M, Takayama T.  High antitumor activity of pladienolide B and its derivative in gastric cancer.  Cancer Sci  2014  105(1):110-6  PubMed ID: 24635824   DOI: 10.1111/cas.12317

5870  Takahashi N, Fukushima T, Yorita K, Tanaka H, Chijiiwa K, Kataoka H.  Dickkopf-1 is overexpressed in human pancreatic ductal adenocarcinoma cells and is involved in invasive growth.  Int J Cancer.  2010  126(7):1611-20  PubMed ID: 19711349   DOI: 10.1002/ijc.24865