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Cell No. : Cell Name
RCB1021 : T3M-4  update : 2024/07/08
CommentPancreatic adenocarcinoma producing CEA. K-ras activated.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Okabe, Tetsuro
Originator Okabe, Tetsuro
Year of deposit 1994
Another name PANC
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 64 years
Tissue pancreas, lymph node meta
Metastatic ability Yes
Classification cancer
Year of origin 1978
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4056
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF10 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 3 split
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 57-130(50) : /58(4),59(5),60(10),61(8),62(7)/
STR(human) OK
deposit info
lot info
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
11 10,13 12 11,12 18,19,21 9.3 X 11,12 10
Images
deposit info
lot info
Reference information Reference 2
User's Publication 24


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Reference
2023  Hirai H, Okabe T, Anraku Y, Fujisawa M, Urabe A, Takaku F  Activation of the c-K-ras oncogene in a human pancreas carcinoma.  Biochem Biophys Res Commun  1985  127:168-74  PubMed ID: 3855240   DOI: 10.1016/s0006-291x(85)80140-6
2022  Okabe T, Yamaguchi N, Ohsawa N  Establishment and characterization of a carcinoembryonic antigen (CEA)-producing cell line from a human carcinoma of the exocrine pancreas.  Cancer  1983  51:662-8  PubMed ID: 6821838   DOI: 10.1002/1097-0142(19830215)51:4<662::aid-cncr2820510419>3.0.co;2-x

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User's Publication
19584  Kondo N, Takada S, Hagimori M, Temma T.  Development of a 2-(2-Hydroxyphenyl)-1 H-benzimidazole-Based Fluorescence Sensor Targeting Boronic Acids for Versatile Application in Boron Neutron Capture Therapy  Cancers (Basel)  2023  15(6):1862  PubMed ID: 36980747   DOI: 10.3390/cancers15061862
19685  Sakabe N, Maruyama S, Ito C, Shimoyama Y, Komene T, Oboshi W, Sato S, Sudo K, Ikeda K.  Immunocytochemical Reactivity Comparison between Formalin-Fixed and Liquid-Based Cytology-Fixed Specimens  Acta Cytol  2023  67(1):38-45  PubMed ID: 36228592   DOI: 10.1159/000526131
19807  Shichi Y, Gomi F, Hasegawa Y, Nonaka K, Shinji S, Takahashi K, Ishiwata T.  Artificial intelligence-based analysis of time-lapse images of sphere formation and process of plate adhesion and spread of pancreatic cancer cells  Front Cell Dev Biol  2023  11:1290753  PubMed ID: 38046666   DOI: 10.3389/fcell.2023.1290753
19924  Yoo HB, Moon JW, Kim HR, Lee HS, Miyabayashi K, Park CH, Ge S, Zhang A, Tae YK, Sub Y, Park HW, Gee HY, Notta F, Tuveson DA, Bang S, Kim MY, Roe JS.  A TEAD2-Driven Endothelial-Like Program Shapes Basal-Like Differentiation and Metastasis of Pancreatic Cancer  Gastroenterology  2023  165(1):133-148.e17  PubMed ID: 36907523   DOI: 10.1053/j.gastro.2023.02.049
21264  Sakabe N, Maruyama S, Ito C, Shimoyama Y, Sudo K, Sato S, Ikeda K.  Effect of liquid-based cytology fixing solution on immunocytochemistry: Efficacy of antigen retrieval in cytologic specimens.  Diagn Cytopathol  2023    PubMed ID: 37329327   DOI: 10.1002/dc.25178
21476  Hirano F, Kondo N, Murata Y, Sudani A, Temma T.  Assessing the effectiveness of fluorinated and α-methylated 3-boronophenylalanine for improved tumor-specific boron delivery in boron neutron capture therapy.  Bioorg Chem  2023  142:106940  PubMed ID: 37939508   DOI: 10.1016/j.bioorg.2023.106940
17663  Kondo N, Aoki E, Takada S, Temma T.  A Red-Emitting Fluorescence Sensor for Detecting Boronic Acid-Containing Agents in Cells  Sensors (Basel)  2022  22(19):7671  PubMed ID: 36236770   DOI: 10.3390/s22197671
18221  Shichi Y, Gomi F, Sasaki N, Nonaka K, Arai T, Ishiwata T.  Epithelial and Mesenchymal Features of Pancreatic Ductal Adenocarcinoma Cell Lines in Two- and Three-Dimensional Cultures  J Pers Med  2022  12(5):746  PubMed ID: 35629168   DOI: 10.3390/jpm12050746
18227  Chen Y, Yang S, Tavormina J, Tampe D, Zeisberg M, Wang H, Mahadevan KK, Wu CJ, Sugimoto H, Chang CC, Jenq RR, McAndrews KM, Kalluri R.  Oncogenic collagen I homotrimers from cancer cells bind to α3β1 integrin and impact tumor microbiome and immunity to promote pancreatic cancer  Cancer Cell  2022  40(8):818-834.e9  PubMed ID: 35868307   DOI: 10.1016/j.ccell.2022.06.011
20712  Kondo N, Hirano F, Temma T.  Evaluation of 3-Borono-l-Phenylalanine as a Water-Soluble Boron Neutron Capture Therapy Agent.  Pharmaceutics  2022  14(5)  PubMed ID: 35631692   DOI: 10.3390/pharmaceutics14051106
20090  Minami F, Sasaki N, Shichi Y, Gomi F, Michishita M, Ohkusu-Tsukada K, Toyoda M, Takahashi K, Ishiwata T.  Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures.  Sci Rep  2021  11(1):6775  PubMed ID: 33762591   DOI: 10.1038/s41598-021-86028-1
12080  Nishigaki T, Takahashi T, Serada S, Fujimoto M, Ohkawara T, Hara H, Sugase T, Otsuru T, Saito Y, Tsujii S, Nomura T, Tanaka K, Miyazaki Y, Makino T, Kurokawa Y, Nakajima K, Eguchi H, Yamasaki M, Mori M, Doki Y, Naka T.  Anti-glypican-1 antibody-drug conjugate is a potential therapy against pancreatic cancer.  Br. J. Cancer  2020    PubMed ID: 32152502   DOI: 10.1038/s41416-020-0781-2
13287  Sasaki N, Gomi F, Yoshimura H, Yamamoto M, Matsuda Y, Michishita M, Hatakeyama H, Kawano Y, Toyoda M, Korc M, Ishiwata T.  FGFR4 Inhibitor BLU9931 Attenuates Pancreatic Cancer Cell Proliferation and Invasion While Inducing Senescence: Evidence for Senolytic Therapy Potential in Pancreatic Cancer.  Cancers (Basel)  2020    PubMed ID: 33066597   DOI: 10.3390/cancers12102976
14280  Hobbs GA, Baker NM, Miermont AM, Thurman RD, Pierobon M, Tran TH, Anderson AO, Waters AM, Diehl JN, Papke B, Hodge RG, Klomp JE, Goodwin CM, DeLiberty JM, Wang J, Ng RWS, Gautam P, Bryant KL, Esposito D, Campbell SL, Petricoin EF 3rd, Simanshu DK, Aguirre AJ, Wolpin BM, Wennerberg K, Rudloff U, Cox AD, Der CJ.  Atypical KRAS G12R Mutant Is Impaired in PI3K Signaling and Macropinocytosis in Pancreatic Cancer  Cancer Discov  2020  10(1):104-123  PubMed ID: 31649109   DOI: 10.1158/2159-8290.CD-19-1006
11646  Shichi Y, Sasaki N, Michishita M, Hasegawa F, Matsuda Y, Arai T, Gomi F, Aida J, Takubo K, Toyoda M, Yoshimura H, Takahashi K, Ishiwata T.  Enhanced morphological and functional differences of pancreatic cancer with epithelial or mesenchymal characteristics in 3D culture.  Sci Rep  2019    PubMed ID: 31350453   DOI: 10.1038/s41598-019-47416-w
11960  Sasaki N, Hirabayashi K, Michishita M, Takahashi K, Hasegawa F, Gomi F, Itakura Y, Nakamura N, Toyoda M, Ishiwata T.  Ganglioside GM2, highly expressed in the MIA PaCa-2 pancreatic ductal adenocarcinoma cell line, is correlated with growth, invasion, and advanced stage.  Sci Rep  2019    PubMed ID: 31852956   DOI: 10.1038/s41598-019-55867-4
14277  Liu S, Hausmann S, Carlson SM, Fuentes ME, Francis JW, Pillai R, Lofgren SM, Hulea L, Tandoc K, Lu J, Li A, Nguyen ND, Caporicci M, Kim MP, Maitra A, Wang H, Wistuba II, Porco JA Jr, Bassik MC, Elias JE, Song J, Topisirovic I, Van Rechem C, Mazur PK, Gozani O.  METTL13 Methylation of eEF1A Increases Translational Output to Promote Tumorigenesis  Cell  2019  176(3):491-504.e21  PubMed ID: 30612740   DOI: 10.1016/j.cell.2018.11.038
4544  Kamerkar S, LeBleu VS, Sugimoto H, Yang S, Ruivo CF, Melo SA, Lee JJ, Kalluri R.  Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer.  Nature  2017  546(7659):498-503  PubMed ID: 28607485   DOI: 10.1038/nature22341
14928  Melo SA, Luecke LB, Kahlert C, Fernandez AF, Gammon ST, Kaye J, LeBleu VS, Mittendorf EA, Weitz J, Rahbari N, Reissfelder C, Pilarsky C, Fraga MF, Piwnica-Worms D, Kalluri R.  Glypican-1 identifies cancer exosomes and detects early pancreatic cancer  Nature  2015  523(7559):177-82  PubMed ID: 26106858   DOI: 10.1038/nature14581
5590  Wongthai P, Hagiwara K, Miyoshi Y, Wiriyasermkul P, Wei L, Ohgaki R, Kato I, Hamase K, Nagamori S, Kanai Y.  Boronophenylalanine, a boron delivery agent for boron neutron capture therapy, is transported by ATB0,+, LAT1 and LAT2  Cancer Sci  2015  106(3):279-86  PubMed ID: 25580517   DOI: 10.1111/cas.12602
18173  Kahlert C, Melo SA, Protopopov A, Tang J, Seth S, Koch M, Zhang J, Weitz J, Chin L, Futreal A, Kalluri R.  Identification of double-stranded genomic DNA spanning all chromosomes with mutated KRAS and p53 DNA in the serum exosomes of patients with pancreatic cancer  J Biol Chem  2014  289(7):3869-75  PubMed ID: 24398677   DOI: 10.1074/jbc.C113.532267
7761  Kahlert C, Bergmann F, Beck J, Welsch T, Mogler C, Herpel E, Dutta S, Niemietz T, Koch M, Weitz J.  Low expression of aldehyde dehydrogenase 1A1 (ALDH1A1) is a prognostic marker for poor survival in pancreatic cancer.  BMC Cancer  2011  11:275  PubMed ID: 21708005   DOI: 10.1186/1471-2407-11-275
5877  Yamamoto H, Okumura K, Toshima S, Mukaisho K, Sugihara H, Hattori T, Kato M, Asano S.  FXYD3 protein involved in tumor cell proliferation is overproduced in human breast cancer tissues.  Biol Pharm Bull  2009  32(7):1148-54  PubMed ID: 19571376   DOI: 10.1248/bpb.32.1148
3717  Wada M, Yazumi S, Takaishi S, Hasegawa K, Sawada M, Tanaka H, Ida H, Sakakura C, Ito K, Ito Y, Chiba T.  Frequent loss of RUNX3 gene expression in human bile duct and pancreatic cancer cell lines.  Oncogene  2004  23(13):2401-7  PubMed ID: 14743205   DOI: 10.1038/sj.onc.1207395



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