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Cell No. : Cell Name
RCB1021 : T3M-4  update : 2022/03/28
CommentPancreatic adenocarcinoma producing CEA. K-ras activated.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Okabe, Tetsuro
Originator Okabe, Tetsuro
Year of deposit 1994
Another name PANC
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 64 years
Tissue pancreas, lymph node meta
Metastatic ability Yes
Classification cancer
Year of origin 1978
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4056
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF10 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 3 split
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 57-130(50) : /58(4),59(5),60810),61(8),62(7)/
STR(human) OK
deposit info
lot info
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
11 10,13 12 11,12 18,19,21 9.3 X 11,12 10
Images
deposit info
lot info
Reference information Reference 2
User's Publication 13


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Reference
2023  Hirai H, Okabe T, Anraku Y, Fujisawa M, Urabe A, Takaku F  Activation of the c-K-ras oncogene in a human pancreas carcinoma.  Biochem Biophys Res Commun  1985  127:168-74  PubMed ID: 3855240
2022  Okabe T, Yamaguchi N, Ohsawa N  Establishment and characterization of a carcinoembryonic antigen (CEA)-producing cell line from a human carcinoma of the exocrine pancreas.  Cancer  1983  51:662-8  PubMed ID: 6821838

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User's Publication
20712  Kondo N, Hirano F, Temma T.  Evaluation of 3-Borono-l-Phenylalanine as a Water-Soluble Boron Neutron Capture Therapy Agent.  Pharmaceutics  2022  14(5)  PubMed ID: 35631692
20090  Minami F, Sasaki N, Shichi Y, Gomi F, Michishita M, Ohkusu-Tsukada K, Toyoda M, Takahashi K, Ishiwata T.  Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures.  Sci Rep  2021  11(1):6775  PubMed ID: 33762591
12080  Nishigaki T, Takahashi T, Serada S, Fujimoto M, Ohkawara T, Hara H, Sugase T, Otsuru T, Saito Y, Tsujii S, Nomura T, Tanaka K, Miyazaki Y, Makino T, Kurokawa Y, Nakajima K, Eguchi H, Yamasaki M, Mori M, Doki Y, Naka T.  Anti-glypican-1 antibody-drug conjugate is a potential therapy against pancreatic cancer.  Br. J. Cancer  2020    PubMed ID: 32152502
13287  Sasaki N, Gomi F, Yoshimura H, Yamamoto M, Matsuda Y, Michishita M, Hatakeyama H, Kawano Y, Toyoda M, Korc M, Ishiwata T.  FGFR4 Inhibitor BLU9931 Attenuates Pancreatic Cancer Cell Proliferation and Invasion While Inducing Senescence: Evidence for Senolytic Therapy Potential in Pancreatic Cancer.  Cancers (Basel)  2020    PubMed ID: 33066597
14280  Hobbs GA, Baker NM, Miermont AM, Thurman RD, Pierobon M, Tran TH, Anderson AO, Waters AM, Diehl JN, Papke B, Hodge RG, Klomp JE, Goodwin CM, DeLiberty JM, Wang J, Ng RWS, Gautam P, Bryant KL, Esposito D, Campbell SL, Petricoin EF 3rd, Simanshu DK, Aguirre AJ, Wolpin BM, Wennerberg K, Rudloff U, Cox AD, Der CJ.  Atypical KRAS G12R Mutant Is Impaired in PI3K Signaling and Macropinocytosis in Pancreatic Cancer  Cancer Discov  2020  10(1):104-123  PubMed ID: 31649109
11646  Shichi Y, Sasaki N, Michishita M, Hasegawa F, Matsuda Y, Arai T, Gomi F, Aida J, Takubo K, Toyoda M, Yoshimura H, Takahashi K, Ishiwata T.  Enhanced morphological and functional differences of pancreatic cancer with epithelial or mesenchymal characteristics in 3D culture.  Sci Rep  2019    PubMed ID: 31350453
11960  Sasaki N, Hirabayashi K, Michishita M, Takahashi K, Hasegawa F, Gomi F, Itakura Y, Nakamura N, Toyoda M, Ishiwata T.  Ganglioside GM2, highly expressed in the MIA PaCa-2 pancreatic ductal adenocarcinoma cell line, is correlated with growth, invasion, and advanced stage.  Sci Rep  2019    PubMed ID: 31852956
14277  Liu S, Hausmann S, Carlson SM, Fuentes ME, Francis JW, Pillai R, Lofgren SM, Hulea L, Tandoc K, Lu J, Li A, Nguyen ND, Caporicci M, Kim MP, Maitra A, Wang H, Wistuba II, Porco JA Jr, Bassik MC, Elias JE, Song J, Topisirovic I, Van Rechem C, Mazur PK, Gozani O.  METTL13 Methylation of eEF1A Increases Translational Output to Promote Tumorigenesis  Cell  2019  176(3):491-504.e21  PubMed ID: 30612740
4544  Kamerkar S, LeBleu VS, Sugimoto H, Yang S, Ruivo CF, Melo SA, Lee JJ, Kalluri R.  Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer.  Nature  2017  546(7659):498-503  PubMed ID: 28607485
14928  Melo SA, Luecke LB, Kahlert C, Fernandez AF, Gammon ST, Kaye J, LeBleu VS, Mittendorf EA, Weitz J, Rahbari N, Reissfelder C, Pilarsky C, Fraga MF, Piwnica-Worms D, Kalluri R.  Glypican-1 identifies cancer exosomes and detects early pancreatic cancer  Nature  2015  523(7559):177-82  PubMed ID: 26106858
7761  Kahlert C, Bergmann F, Beck J, Welsch T, Mogler C, Herpel E, Dutta S, Niemietz T, Koch M, Weitz J.  Low expression of aldehyde dehydrogenase 1A1 (ALDH1A1) is a prognostic marker for poor survival in pancreatic cancer.  BMC Cancer  2011  11:275  PubMed ID: 21708005
5877  Yamamoto H, Okumura K, Toshima S, Mukaisho K, Sugihara H, Hattori T, Kato M, Asano S.  FXYD3 protein involved in tumor cell proliferation is overproduced in human breast cancer tissues.  Biol Pharm Bull  2009  32(7):1148-54  PubMed ID: 19571376
3717  Wada M, Yazumi S, Takaishi S, Hasegawa K, Sawada M, Tanaka H, Ida H, Sakakura C, Ito K, Ito Y, Chiba T.  Frequent loss of RUNX3 gene expression in human bile duct and pancreatic cancer cell lines.  Oncogene  2004  23(13):2401-7  PubMed ID: 14743205



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