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Cell No. : Cell Name
RCB1021 : T3M-4  update : 2020/09/24
CommentPancreatic adenocarcinoma producing CEA. K-ras activated. Cell growth is slow.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Okabe, Tetsuro
Originator Okabe, Tetsuro
Year of deposit 1994
Another name PANC
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 64 years
Tissue pancreas, lymph node meta
Metastastatic ability Yes
Classification cancer
Year of origin 1978
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF10 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 3 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 57-130(50) : /58(4),59(5),60810),61(8),62(7)/
deposit info
lot info
Reference information Reference 2
User's Publication 7

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2023  Hirai H, Okabe T, Anraku Y, Fujisawa M, Urabe A, Takaku F  Activation of the c-K-ras oncogene in a human pancreas carcinoma.  Biochem Biophys Res Commun  1985  127:168-74  PubMed ID: 3855240
2022  Okabe T, Yamaguchi N, Ohsawa N  Establishment and characterization of a carcinoembryonic antigen (CEA)-producing cell line from a human carcinoma of the exocrine pancreas.  Cancer  1983  51:662-8  PubMed ID: 6821838

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User's Publication
12080  Nishigaki T, Takahashi T, Serada S, Fujimoto M, Ohkawara T, Hara H, Sugase T, Otsuru T, Saito Y, Tsujii S, Nomura T, Tanaka K, Miyazaki Y, Makino T, Kurokawa Y, Nakajima K, Eguchi H, Yamasaki M, Mori M, Doki Y, Naka T.  Anti-glypican-1 antibody-drug conjugate is a potential therapy against pancreatic cancer.  Br. J. Cancer  2020    PubMed ID: 32152502
11646  Shichi Y, Sasaki N, Michishita M, Hasegawa F, Matsuda Y, Arai T, Gomi F, Aida J, Takubo K, Toyoda M, Yoshimura H, Takahashi K, Ishiwata T.  Enhanced morphological and functional differences of pancreatic cancer with epithelial or mesenchymal characteristics in 3D culture.  Sci Rep  2019    PubMed ID: 31350453
11960  Sasaki N, Hirabayashi K, Michishita M, Takahashi K, Hasegawa F, Gomi F, Itakura Y, Nakamura N, Toyoda M, Ishiwata T.  Ganglioside GM2, highly expressed in the MIA PaCa-2 pancreatic ductal adenocarcinoma cell line, is correlated with growth, invasion, and advanced stage.  Sci Rep  2019    PubMed ID: 31852956
4544  Kamerkar S, LeBleu VS, Sugimoto H, Yang S, Ruivo CF, Melo SA, Lee JJ, Kalluri R.  Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer.  Nature  2017  546(7659):498-503  PubMed ID: 28607485
7761  Kahlert C, Bergmann F, Beck J, Welsch T, Mogler C, Herpel E, Dutta S, Niemietz T, Koch M, Weitz J.  Low expression of aldehyde dehydrogenase 1A1 (ALDH1A1) is a prognostic marker for poor survival in pancreatic cancer.  BMC Cancer  2011  11:275  PubMed ID: 21708005
5877  Yamamoto H, Okumura K, Toshima S, Mukaisho K, Sugihara H, Hattori T, Kato M, Asano S.  FXYD3 protein involved in tumor cell proliferation is overproduced in human breast cancer tissues.  Biol Pharm Bull  2009  32(7):1148-54  PubMed ID: 19571376
3717  Wada M, Yazumi S, Takaishi S, Hasegawa K, Sawada M, Tanaka H, Ida H, Sakakura C, Ito K, Ito Y, Chiba T.  Frequent loss of RUNX3 gene expression in human bile duct and pancreatic cancer cell lines.  Oncogene  2004  23(13):2401-7  PubMed ID: 14743205

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