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Cell No. : Cell Name
RCB1020 : T3M-10  update : 2024/07/05
CommentLung large cell carcinoma producing CSF.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Okabe, Tetsuro
Originator Okabe, Tetsuro
Year of deposit 1994
Another name TT
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 40 years
Tissue lung, lymph node meta
Disease name large cell carcinoma
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8067
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF10 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 41-48(34) : 41(1),42(14),43(8),44(4),45(1),46(4)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
2026  Okabe T, Fujisawa M, Kudo H, Honma H, Ohsawa N, Takaku F  Establishment of a human colony-stimulating-factor-producing cell line from an undifferentiated large cell carcinoma of the lung.  Cancer  1984  54:1024-9  PubMed ID: 6088016   DOI: 10.1002/1097-0142(19840915)54:6<1024::aid-cncr2820540616>3.0.co;2-1

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User's Publication
19029  Kanamori-Katayama M, Kaiho A, Ishizu Y, Okamura-Oho Y, Hino O, Abe M, Kishimoto T, Sekihara H, Nakamura Y, Suzuki H, Forrest AR, Hayashizaki Y.  LRRN4 and UPK3B are markers of primary mesothelial cells  PLoS One  2011  6(10):e25391  PubMed ID: 21984916   DOI: 10.1371/journal.pone.0025391



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