Cell No. : Cell Name
RCB1018 : T3M-3
update : 2024/11/12
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| Comment | Trophoblastic cell. Producing chorionic gonadotropin, estrogen, progesterone. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Okabe, Tetsuro
|
| Originator |
Okabe, Tetsuro
|
| Year of deposit |
1994
|
| Another name |
Chorio
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Tissue |
choriocarcinoma
|
| Classification |
cancer
|
| Year of origin |
1978
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_8065
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
HamF10 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : once/2-3 weeks, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
63-71(50) : /64(3),65(8),66(9),67(11),68(10),69(4)/
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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|
| Reference information |
Reference |
3
|
| User's Publication |
1
|
| Reference |
|
5478
Okabe T, Ishizaka S, Fujisawa M.
Human trophoblastic cells metabolize 25-hydroxyvitamin D3 in vitro.
Jpn J Med
1991
30(4):326-9
PubMed ID: 1658420
DOI: 10.2169/internalmedicine1962.30.326
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|
2021
Uzumaki H, Okabe T, Sasaki N, Hagiwara K, Takaku F, Tobita M, Yasukawa K, Ito S, Umezawa Y
Identification and characterization of receptors for granulocyte colony-stimulating factor on human placenta and trophoblastic cells.
Proc Natl Acad Sci U S A
1989
86:9323-6
PubMed ID: 2480598
DOI: 10.1073/pnas.86.23.9323
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2020
Okabe T, Sasaki N, Matsuzaki M, Imai Y, Kaneko Y, Matsuzaki F, Takaku F, Tsushima T
Establishment and characterization of a new human functional cell line from a choriocarcinoma.
Cancer Res
1983
43:4920-6
PubMed ID: 6603906
|
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